1887

Abstract

A proteinase has been purified from the stipes of senescent sporophores of the mushroom The proteinase was inhibited by PMSF. It has a broad pH optimum, 6·5–11·5, and a narrow substrate specificity, requiring both a hydrophobic amino acid in the P position and a minimum peptide chain length. The apparent molecular mass of the proteinase was 27 kDa when determined by SDS-PAGE and 14·1 kDa when measured by gel filtration. The isoelectric point of the proteinase was 9·0. Polyclonal antibodies have been raised to the proteinase. The proteinase from has similar properties to, and 60% N-terminal sequence identity with, proteinase K from the fungus

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1993-06-01
2021-05-11
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