1887

Abstract

A proteinase has been purified from the stipes of senescent sporophores of the mushroom The proteinase was inhibited by PMSF. It has a broad pH optimum, 6·5–11·5, and a narrow substrate specificity, requiring both a hydrophobic amino acid in the P position and a minimum peptide chain length. The apparent molecular mass of the proteinase was 27 kDa when determined by SDS-PAGE and 14·1 kDa when measured by gel filtration. The isoelectric point of the proteinase was 9·0. Polyclonal antibodies have been raised to the proteinase. The proteinase from has similar properties to, and 60% N-terminal sequence identity with, proteinase K from the fungus

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-6-1379
1993-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/6/mic-139-6-1379.html?itemId=/content/journal/micro/10.1099/00221287-139-6-1379&mimeType=html&fmt=ahah

References

  1. Bajorath J., Saenger W., Pal G. P. 1988; Autolysis and inhibition of proteinase K, a subtilisin-related serine proteinase isolated from the fungus Tritirachium album Limber.. Biochimica et Biophysica Acta 954:176–182
    [Google Scholar]
  2. Bradford M. M. 1976; A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding.. Analytical Biochemistry 72:248–254
    [Google Scholar]
  3. Burton K. S. 1988a; The effects of storage and development on Agaricus bisporus proteases.. Journal of Horticultural Science 63:103–108
    [Google Scholar]
  4. Burton K. S. 1988b; The effects of pre- and post-harvest development on mushroom tyrosinase.. Journal of Horticultural Science 63:255–260
    [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programmes for the VAX.. Nucleic Acids Research 12:387–396
    [Google Scholar]
  6. Ebeling W., Hennrich N., Klockow M., Metz H., Orth H. D., Lang H. 1974; Proteinase K from Tritirachium album Limber.. European Journal of Biochemistry 47:91–97
    [Google Scholar]
  7. Fermor T. R., Wood D. A. 1981; Degradation of bacteria by Agaricus bisporus and other fungi.. Journal of General Microbiology 126:377–387
    [Google Scholar]
  8. Golovchenko N. P., Kataeva I. A., Akimenko V. K. 1992; Analysis of pH-dependent protein interactions with gel filtration medium.. Journal of Chromatography 591:121–128
    [Google Scholar]
  9. Hammond J. B. W. 1978; Carbohydrate catabolism in harvested mushrooms.. Phytochemistry 17:1717–1719
    [Google Scholar]
  10. Hammond J. B. W., Nichols R. 1975; Changes in respiration and soluble carbohydrates during the post-harvest storage of mushrooms (Agaricus bisporus).. Journal of the Science of Food and Agriculture 26:835–842
    [Google Scholar]
  11. Hammond J. B. W., Nichols R. 1976; Carbohydrate metabolism in Agaricus bisporus (Lange) Sing.: changes in soluble carbohydrates during growth of mycelium and sporophore.. Journal of General Microbiology 93:309–320
    [Google Scholar]
  12. Hänseler E., Nylén L. E., Rast D. M. 1983; Isolation and properties of chitin synthase from Agaricus bisporus mycelium.. Experimental Mycology 7:17–30
    [Google Scholar]
  13. Harlow E., Lane D. P. 1988; Immunoassay.. In Antibodies: a Laboratory Manual pp. 562–577 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  14. Hunkapillar M. W., Hewick R. E., Dreyer W. J., Hood L. E. 1983; High sensitivity sequencing with a gas phase sequenator.. Methods in Enzymology 91:399–413
    [Google Scholar]
  15. Jany K.-D., Mayer B. 1985; Proteinase K from Tritirachium album Limber. I. Molecular mass and sequence around the active site serine residue.. Biological Chemistry Hoppe-Seyler 366:485–492
    [Google Scholar]
  16. Jany K. D., Lederer G., Mayer B. 1986; Amino acid sequence of proteinase K from the mold Tritirachium album Limber. Proteinase K - a subtilin-related enzyme with disulphide bonds.. FEBS Letters 199:139–144
    [Google Scholar]
  17. Kalisz H. M., Wood D. A., Moore D. 1987; Production, regulation and release of extracellular proteinase activity in basidiomycete fungi.. Transactions of the British Mycological Society 88:221–227
    [Google Scholar]
  18. Kizuki K., Moriya H. 1982; A new potent kinin-inactivating enzyme from mushroom, Psalliota hortensis.. Chemical Pharmaceutical Bulletin (Tokyo) 30:2241–2244
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature; London: 227:680–685
    [Google Scholar]
  20. Moehle C. M, Tizard R., Lemmon S. K., Smart J., Jones E. W. 1987; Protease B of the lysosome-like vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases.. Molecular and Cell Biology 7:4390–4399
    [Google Scholar]
  21. Murr D. P., Morris L. L. 1975; Effect of storage temperature on post-harvest changes in mushrooms.. Journal of the American Society of Horticultural Science 100:16–19
    [Google Scholar]
  22. North M. J. 1982; Comparative biochemistry of the proteinases of eucaryotic microorganisms.. Microbiological Reviews 46:308–340
    [Google Scholar]
  23. Pähler A., Banerjee A., Dattagupta J. K., Fujiwara T., Lindner K., Pal G. P., Suck D., Weber G., Saenger W. 1984; Three-dimensional structure of fungal proteinase K reveals similarity to bacterial subtilisin.. EMBO Journal 3:1311–1314
    [Google Scholar]
  24. Samal B. B., Karan B., Boone T. C., Chen K. K., Rohde M. F., Stabinsky Y. 1989; Cloning and expression of the genes encoding a novel proteinase from Tritirachium album Limber.. Gene 85:329–333
    [Google Scholar]
  25. Spencer D. M. 1985; The mushroom, its history and importance.. In The Biology and Technology of the Cultivated Mushroom pp. 1–8 Edited by Flegg P. B., Spencer D. M., Wood D. A. Chichester: Wiley-Interscience;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-6-1379
Loading
/content/journal/micro/10.1099/00221287-139-6-1379
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error