1887

Abstract

Phosphofructokinase II (PFK II) from was partially purified (247-fold). The calculated values for fructose 6-phosphate and ATP were 0·7 m and 40 μ, respectively. Upon incubation in the presence of [γ-P]ATP, the enzyme formed a radioactive phosphoprotein with molecular mass of 67 kDa in autoradiography analysis after SDS-PAGE. Upon incubation in the presence of ATP-Mg and the catalytic subunit of cAMP-dependent protein kinase, its activity was not modified. The same result was obtained when a cell-free extract of was incubated with ATP-Mg and cAMP. 2,4-Dinitrophenol caused a transient rise in cAMP levels in the fungal cell. These results provide evidence that the fructose 2,6-bisphosphate level in is independent of cAMP concentrations and not related to a cAMP-dependent mechanism, but to the availability of substrate fructose 6-phosphate.

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1993-06-01
2021-07-25
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References

  1. Abraham G. N., Scaletta C., Vaughan J. H. 1972; Modified diphenylamine reaction for increased sensitivity.. Analytical Biochemistry 49:547–549
    [Google Scholar]
  2. Ann Dumbrava V., Pall M. L. 1987; Regulation of fructose 2,6-bisphosphate levels in Neurospora crassa. . Biochimica et Biophysica Acta 925:210–217
    [Google Scholar]
  3. Aragón J. J., Sanchez V., Boto L. 1986; Fructose 2,6-bisphosphate in Dictyostelium discoideum. Independence of cyclic AMP production and inhibition of fructose-1,6-bisphosphatase.. European Journal of Biochemistry 161:757–761
    [Google Scholar]
  4. Bazan J. F., Fletterick R. J., Pilkis S. J. 1989; Evolution of a bifunctional enzyme; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.. Proceedings of the National Academy of Sciences of the United States of America 86:9642–9646
    [Google Scholar]
  5. Béguin P. 1990; Molecular biology of cellulose degradation.. Annual Review of Microbiology 44:219–248
    [Google Scholar]
  6. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.. Analytical Biochemistry 72:248–254
    [Google Scholar]
  7. El-Gogary S., Leite A., Crivellaro O., Eveleigh D., El-Dorry H. 1989; Mechanism by which cellulose triggers cellobiohydrolaseI gene expression in Trichoderma reesei. . Proceedings of the National Academy of Sciences of the United States of America 86:6138–6141
    [Google Scholar]
  8. El-Maghrabi M. R., Claus T. H., Pilkis J., Pilkis S. J. 1981; Partial purification of a rat liver enzyme that catalyses the formation of fructose 2,6-bisphosphate.. Biochemical and Biophysical Research Communications 101:1071–1077
    [Google Scholar]
  9. El-Maghrabi M. R., Fox E., Pilkis J., Pilkis S. J. 1982; Cyclic AMP-dependent phosphorylation of rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase.. Biochemical and Biophysical Research Communications 106:794–802
    [Google Scholar]
  10. François J. M., Van Schaftingen E., Hers H. G. 1984; The mechanism by which glucose increases fructose 2,6-bisphosphate concentration in Saccharomyces cerevisiae. A cyclic AMP-dependent activation of phosphofructokinase 2.. European Journal of Biochemistry 145:187–193
    [Google Scholar]
  11. François J. M., Van Schaftingen E., Hers H. G. 1988; Characterization of phosphofructokinase 2 and of enzymes involved in the degradation of fructose 2,6-bisphosphate in yeast.. European Journal of Biochemistry 171:599–608
    [Google Scholar]
  12. Furuya E., Uyeda K. 1981; A novel enzyme catalyses the synthesis of activation factor from ATP and D-fructose-6-P.. Journal of Biological Chemistry 2556:7109–7112
    [Google Scholar]
  13. Furuya E., Yokoyama M., Uyeda K. 1982; Regulation of fructose-6-phosphate 2-kinase by phosphorylation and dephosphorylation: possible mechanism of coordinated control of glycolysis and glycogenolysis.. Proceedings of the National Academy of Sciences of the United States of America 79:325–329
    [Google Scholar]
  14. Hue L., Blackmore P. F., Extron J. H. 1981; Fructose 2,6-bisphosphate-hormonal regulation and mechanism of its formation in liver.. Journal of Biological Chemistry 256:8900–8903
    [Google Scholar]
  15. Jaynes P. K., Mcdonough J. P., Mahler H. R. 1982; Properties and possible functions of the adenylate cyclase in plasma membranes of Saccharomyces cerevisiae. . Molecular and Cellular Biology 2:1481–1491
    [Google Scholar]
  16. Kitamura K., Uyeda K., Kangawa K., Matsuo K. 1989; Purification and characterization of rat skeletal muscle fructose-6- phosphate 2-kinase:fructose-2,6-bisphosphatase.. Journal of Biological Chemistry 264:9799–9806
    [Google Scholar]
  17. Kretschmer M., Schellenberg W., Otto A., Kessler R., Hofmann E. 1987; Fructose-2,6-bisphosphatase and 6-phosphofructo 2-kinase are separable in yeast.. Biochemical Journal 246:755–759
    [Google Scholar]
  18. Larondelle Y., Mertens E., Van Schaftingen E., Hers H. G. 1986; Purification and properties of spinach leaf phosphofructokinase 2/fructose-2,6-bisphosphatase.. European Journal of Biochemistry 161:351–357
    [Google Scholar]
  19. Leite A., Abrahão Neto J., Leyton J. F., Crivelaro O., El-Dorry H. 1988; Phosphofructokinase from bumblebee flight muscle.. Journal of Biological Chemistry 263:17527–17533
    [Google Scholar]
  20. Montenecourt B. S., Eveleigh D. E. 1979; Selective screening methods for the isolation of high yielding cellulase mutants of Trichoderma reesei. . Advances in Chemistry Series 181:289–301
    [Google Scholar]
  21. Pall M. L. 1984; Is there a general paradigm of cyclic AMP action in eukaryotes?. Molecular and Cellular Biochemistry 58:187–191
    [Google Scholar]
  22. Pall M. L., Trevillyan J. M., Hinman N. 1981; Deficient cyclic adenosine 3´,5´-monophosphate control in mutants of two genes of Neurospora crassa . Molecular and Cellular Biology 1:1–8
    [Google Scholar]
  23. Pilkis S. J., Regen D. M., Stewart H. B., Pilkis J., Pate T. M., El-Maghrabi M. R. 1984; Evidence for two catalytic sites on 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase.. Journal of Biological Chemistry 259:949–958
    [Google Scholar]
  24. Trevillyan J. M., Pall M. L. 1979; Control of cyclic adenosine 3´,5´-monophosphate levels by depolarizing agents in fungi.. Journal of Bacteriology 138:397–403
    [Google Scholar]
  25. Uno I., Ishikawa T. 1981; Control of adenosine 3´,5´-monophosphate level and protein phosphorylation by depolarizing agents in Coprinus macrorhizus. . Biochimica et Biophysica Acta 672:108–113
    [Google Scholar]
  26. Van Laere A., Van Schaftingen E., Hers H. G. 1983; Fructose 2,6-bisphosphate and germination of fungal spores.. Proceedings of the National Academy of Sciences of the United States of America 80:6601–6605
    [Google Scholar]
  27. Van Schaftingen E. 1987; Fructose 2,6-bisphosphate.. Advances in Enzymology 59:315–395
    [Google Scholar]
  28. Van Schaftingen E., Hers H. G. 1980; Synthesis of a stimulator of phosphofructokinase most likely fructose 2,6-bisphosphate from phosphoric acid and fructose 6-phosphoric acid.. Biochemical and Biophysical Research Communications 96:1524–1531
    [Google Scholar]
  29. Van Schaftingen E., Hers H. G. 1981; Phosphofructokinase 2 the enzyme that forms fructose 2,6-bisphosphate from fructose 6-phosphate and ATP.. Biochemical and Biophysical Research Communications 101:1078–1084
    [Google Scholar]
  30. Van Schaftingen E., Hers H. G. 1986; Purification and properties of phosphofructokinase 2/fructose 2,6-bisphosphatase from chicken liver and from pigeon muscle.. European Journal of Biochemistry 159:359–365
    [Google Scholar]
  31. Van Schaftingen E., Davies D. R., Hers H. G. 1982a; Fructose 2,6-bisphosphatase from rat liver.. European Journal of Biochemistry 124:143–149
    [Google Scholar]
  32. Van Schaftingen E., Lederer B., Bartrons R., Hers H. G. 1982b; A kinetic study of pyrophosphate: fructose 6-phosphate phosphotransferase from potato tubers.. European Journal of Biochemistry 129:191–195
    [Google Scholar]
  33. Vandercammen A., François J. M., Torres B. B., Maia J.C.C., Hers H. G. 1990; Fructose 2,6-bisphosphate and carbohydrate metabolism during the life cycle of the aquatic fungus Blastocladiella emersonii. . Journal of General Microbiology 136:137–146
    [Google Scholar]
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