1887

Abstract

Cultures of the Gram-positive bacterium sp. strain 7E1C contained up to 300 mg dodecanedioic acid l after growth on dodecane. Small amounts of tetradecanedioic acid (17 to 45 mg l) were produced during growth on tetradecane or methyl tetradecanoate. No dicarboxylic acids were detected after growth on hexadecane, hexadecanoic acid or 16-hydroxyhexadecanoic acid. Studies on the rates of degradation of exogenous dicarboxylic acids showed that this is not a significant factor influencing the accumulation of dodecanedioic and tetradecanedioic acids. The activities and substrate specificities of a number of enzyme activities involved in dicarboxylic acid metabolism were investigated. The specificities of the long-chain acyl-CoA synthetase and thioesterase, alcohol dehydrogenases and -oxidation are consistent with the accumulation of dodecanedioic acid from dodecane and the lack of production of hexadecanedioic acid from hexadecane. The ω-hydroxy fatty acid may occupy a pivotal position in determining whether significant production of dicarboxylic acid occurs with this organism.

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1993-06-01
2024-04-19
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References

  1. Bacchin P., Robertiello A., Viglia A. 1974; Identification of n-decane oxidation products in Corynebacterium cultures by combined gas chromatography-mass spectrometry.. Applied Microbiology 28:737–741
    [Google Scholar]
  2. Blasig R., Schunk W. H., Jockisch W., Franke P., Muller H. G. 1984; Degradation of long-chain n-alkanes by the yeast Lodderomyces elongisporus. I. Products of alkane oxidation in whole cells.. Applied Microbiology and Biotechnology 19:241–246
    [Google Scholar]
  3. Blasig R, Mauersberger S., Riege P., Schunk W. H., Jockisch W., Franke P., Muller H. G. 1988; Degradation of long-chain n-alkanes by the yeast Candida maltosa. II. Oxidation of n-alkanes and intermediates using microsomal membrane fractions.. Applied Microbiology and Biotechnology 28:589–597
    [Google Scholar]
  4. Boulton C., Ratledge C. 1984; The physiology of hydrocarbonutilizing microorganisms.. Enzyme and Fermentation Biotechnology 9:11–77
    [Google Scholar]
  5. Broadway N. M. 1990 Enzymology of dicarboxylic acid metabolism in Corynebacterium sp. strain 7E1C. PhD thesis University of Hull; UK.:
    [Google Scholar]
  6. Broadway N. M., Dickinson F. M., Ratledge C. 1992; Acyl-CoA intermediates of β-oxidation of mono- and dicarboxylic acids by extracts of Corynebacterium sp. strain 7E1C.. Biochemical Journal 285:117–222
    [Google Scholar]
  7. Buhler M., Schindler J. 1984; Aliphatic hydrocarbons.. In Biotechnology 6a pp. 329–385 Rehm H. J., Reed G. Edited by Weinheim: Verlag Chemie.;
    [Google Scholar]
  8. Butte W. 1983; Rapid method for the determination of fatty acid profiles from fats and oils using trimethyl sulphonium hydroxide for transesterification.. Journal of Chromatography 261:142–145
    [Google Scholar]
  9. Casey J., Macrae A. 1992; Biotechnology and the oleochemical industry.. INFORM 3:203–207
    [Google Scholar]
  10. Casey J., Dobb R., Mycock G. 1990; An effective technique for enrichment and isolation of Candida cloacae mutants defective in alkane metabolism.. Journal of General Microbiology 136:1197–1202
    [Google Scholar]
  11. Chan E. C., Kuo J., Lin H. P., Mou D. G. 1991; Stimulation of n-alkane conversion to dicarboxylic acid by organic-solvent- and detergent-treated microbes.. Applied Microbiology and Biotechnology 34:772–777
    [Google Scholar]
  12. Dalziel K. 1962; Kinetic studies of liver alcohol dehydrogenase.. Biochemical Journal 84:244–254
    [Google Scholar]
  13. Du PONT 1973 U.S. Patent 3773621. Process for the biological production of alpha, omega-alkanedioic acids.
    [Google Scholar]
  14. Du PONT 1974 U.S. Patent 3 784445. Culture medium and process for the biological production of alpha, omega-alkanedioic acids.
    [Google Scholar]
  15. Furukawa T., Matsuyoshi T., Kise S. 1986; Selection of high brassylic acid producing strains of Torulopsis Candida by single-cell cloning and by mutants. . Journal of Fermentation Technology 64:97–101
    [Google Scholar]
  16. Hill F. F., Venn I., Lukas K. L. 1986; Studies on the formation of long-chain dicarboxylic acids from pure n-alkanes by a mutant of Candida tropicalis. . Applied Microbiology and Biotechnology 24:168–174
    [Google Scholar]
  17. Kester A. S., Foster J. W. 1963; Diterminal oxidation of long chain alkanes by bacteria.. Journal of Bacteriology 85:859–869
    [Google Scholar]
  18. Klein K., Steinberg R., Feithen B., Overath P. 1971; Fatty acid degradation in Escherichia coli. . European Journal of Biochemistry 19:442–451
    [Google Scholar]
  19. Modrzakowski M. C., Finnerty W. R. 1989; Intermediary metabolism of Acinetobacter grown on dialkyl ethers.. Canadian Journal of Microbiology 35:1031–1036
    [Google Scholar]
  20. Overath P., Raufuss E., Stoffel W., Ecker W. 1967; The induction of the enzymes of fatty acid degradation in Escherichia coli. . Biochemical and Biophysical Research Communications 29:29–33
    [Google Scholar]
  21. Overath P., Pauli G., Schairer H. U. 1969; Fatty acid degradation in Escherichia coli: an inducible acyl-CoA synthetase, the mapping of a/d-mutations and isolation of regulatory mutants.. European Journal of Biochemistry 9:559–574
    [Google Scholar]
  22. Rehm H. J., Reiff T. 1981; Mechanisms and occurrence of microbial oxidation of long-chain alkanes.. Advances in Biochemical Engineering 19:175–215
    [Google Scholar]
  23. Samuel D., Ailhaud G. 1969; Comparative aspects of fatty acid activation in Escherichia coli and Clostridium butyricum. . FEBS Letters 2:213–216
    [Google Scholar]
  24. Shiio J., Uchio R. 1971; Microbial production of long-chain dicarboxylic acids from n-alkanes.. Agricultural and Biological Chemistry 35:2033–2042
    [Google Scholar]
  25. Uchio R., Shiio J. 1972a; Microbial production of long-chain dicarboxylic acids from n-alkanes.. Agricultural and Biological Chemistry 36:426–433
    [Google Scholar]
  26. Uchio R., Shiio J. 1972b; Production of dicarboxylic acids by Candida cloacae mutant unable to assimilate n-alkane.. Agricultural and Biological Chemistry 36:1169–1175
    [Google Scholar]
  27. Uchio R., Shiio J. 1972c; Tetradecane-l,14-dicarboxylic acid production from hexadecane by Candida cloacae. . Agricultural and Biological Chemistry 36:1389–1397
    [Google Scholar]
  28. Vamecq J., De Hoffmann E., Van Hoof F. 1985; The microsomal dicarboxyl-CoA synthetase.. Biochemical Journal 230:683–693
    [Google Scholar]
  29. Webster L. 1969; Acetyl-CoA synthetase.. Methods in Enzymology 13:375–381
    [Google Scholar]
  30. Weeks E., Shapiro M., Burns R. O., Wakil S. J. 1969; Control of fatty acid metabolism: induction of the enzymes of fatty acid oxidation in Escherichia coli. . Journal of Bacteriology 97:827–836
    [Google Scholar]
  31. Yi Z. H., Rehm H. J. 1982a; Metabolic formation of dodecane- dioic acid from n-dodecane by a mutant of Candida tropicalis. . European Journal of Applied Microbiology and Biotechnology 14:254–258
    [Google Scholar]
  32. Yi Z. H., Rehm H. J. 1982b; Degradation pathways from n-tridecane to α,ω-tridecanedioic acid in a mutant of Candida tropicalis. . European Journal of Applied Microbiology and Biotechnology 15:144–146
    [Google Scholar]
  33. Yi Z. H., Rehm H. J. 1982c; A new metabolic pathway from n-dodecane to α,ω-dodecanedioic acid in a mutant of Candida tropicalis. . European Journal of Applied Microbiology and Biotechnology 15:175–179
    [Google Scholar]
  34. Yi Z. H., Rehm H. J. 1988a; Formation and degradation of Δ91,18-octadecanedioic acid from oleic acid by Candida tropicalis. . Applied Microbiology and Biotechnology 28:520–526
    [Google Scholar]
  35. Yi Z. H., Rehm H. J. 1988b; Bioconversion of elaidic acid toΔ9 trans-1,18-octadecenedioic acid by Candida tropicalis. . Applied Microbiology and Biotechnology 29:305–309
    [Google Scholar]
  36. Yuantong C., Xiuzhen H. 1988; Studies on producing hexadecane dicarboxylic acid by fermentation.. Chinese Journal of Biotechnology 4:145–148
    [Google Scholar]
  37. Zhihua Y., Xiuzhen H. 1986; Formation and characterization of long-chain diacyl-CoA synthetase from Candida tropicalis. . Acta Microbiologica Sinica 26:333–340
    [Google Scholar]
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