Summary: Cultures of the Gram-positive bacterium sp. strain 7E1C contained up to 300 mg dodecanedioic acid I after growth on dodecane. Small amounts of tetradecanedioic acid (17 to 45 mg I) were produced during growth on tetradecane or methyl tetradecanoate. No dicarboxylic acids were detected after growth on hexadecane, hexadecanoic acid or 16-hydroxyhexadecanoic acid. Studies on the rates of degradation of exogenous dicarboxylic acids showed that this is not a significant factor influencing the accumulation of dodecanedioic and tetradecanedioic acids. The activities and substrate specificities of a number of enzyme activities involved in dicarboxylic acid metabolism were investigated. The specificities of the long-chain acyl-CoA synthetase and thioesterase, alcohol dehydrogenases and β-oxidation are consistent with the accumulation of dodecanedioic acid from dodecane and the lack of production of hexadecanedioic acid from hexadecane. The ω-hydroxy fatty acid may occupy a pivotal position in determining whether significant production of dicarboxylic acid occurs with this organism.


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