The effect of an agr mutation on expression of type 5 capsular polysaccharide (CP) by Staphylococcus aureus Newman was investigated in different complex and synthetic media. CP expression by the agr mutant was strongly reduced in certain media but slightly in others, indicating that CP synthesis is positively controlled by agr. CP expression occurred in the post-exponential growth phase in both wild-type and mutant strains, suggesting that other regulatory systems could act in conjunction with agr.
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SmithJ. A.,
SeidmanJ. G.,
StruhlK.1987Current Protocols in Molecular Biology New York: John Wiley and Sons;
BaystonR.,
RodgersJ.1990; Production of extra-cellular slime by Staphylococcus epidermidis during stationary phase of growth: its association with adherence to implantable devices. Journal of Clinical Pathology 43:866–870
BoutonnierA.,
NatoF.,
BouvetA.,
LebrunL.,
AudurierA.,
MazieJ. C.,
FournierJ. M.1989; Direct testing of blood cultures for detection of the serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus
. Journal of Clinical Microbiology 27:989–993
CacalanoG.,
KaysM.,
SaimanL.,
PrinceA.1992; Production of the Pseudomonas aeruginosa neuraminidase is increased under hyperosmolar conditions and is regulated by genes involved in alginate expression. Journal of Clinical Investigation 89:1866–1874
CheungA. L.,
KoomeyJ. M.,
ButlerC. A.,
ProjanS. J.,
FischettiV. A.1992; Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr
. Proceedings of the National Academy of Sciences of the United States of America 89:6462–6466
ColemanG.,
AboshkiwaM.,
Al-AniB.1989; The effect of glucose on the expression of extracellular protein genes by Staphylococcus aureus strain V8. FEMS Microbiology Letters 61:247–250
ColemanG.,
JakemanC. M.,
MartinN.1978; Patterns of total extracellular protein secretion by a number of clinically isolated strains of Staphylococcus aureus
. Journal of General Microbiology 107:189–192
DassyB.,
StringfellowW. T.,
LiebM.,
FournierJ. M.1991; Production of type 5 capsular polysaccharide by Staphylococcus aureus grown in a semi-synthetic medium. Journal of General Microbiology 137:1155–1162
DereticV.,
MohrC. D.,
MartinD. W.1991; Mucoid Pseudomonas aeruginosa in cystic fibrosis: signal transduction and histone-line elements in the regulation of bacterial virulence. Molecular Microbiology 5:1577–1583
DonhamM. C.,
HethH. E.,
LeblancP. A.,
SloanG. L.1988; Characteristics of extracellular protein production by Staphylococcus simulans biovar staphylolyticus during aerobic and anaerobic growth. Journal of General Microbiology 134:2615–2621
DuncanJ. L.,
ChoG. J.1971; Production of staphylococcal alpha toxin. I. Relationship between cell growth and toxin formation. Infection and Immunity 4:456–461
EngelsW.,
KampsM. A. F.1982; The nature of the competitive ability of spontaneous staphylocoagulase-negative mutants of Staphylococcus aureus with respect to growth of the parent strains in continuous culture. Antonie van Leeuwenhoek 48:67–83
FattomA.,
SchneersonR.,
SzuS. C.,
VanW. F.,
ShiloachJ.,
KarakawaW. W.,
RobbinsJ. B.1990; Synthesis and immunologic properties in mice of vaccines composed of Staphylococcus aureus type 5 and type 8 capsular polysaccharides conjugated to Pseudomonas aeruginosa exotoxin A. Infection and Immunity 58:2367–2374
FroschM.,
EdwardsU.,
BoussetK.,
KraubeB.,
WeisgerberC.1991; Evidence for a common molecular origin of the capsule gene loci in Gram-negative bacteria expressing group II capsular polysaccharides. Molecular Microbiology 5:1251–1263
HochkeppelH. K.,
BraunD. G.,
VischerW.,
ImmA.,
SutterJ.,
StaeubliU.,
GuggenheimR.,
KaplanE. L.,
BoutonnierA.,
FournierJ. M.1987; Serotyping and electron microscopy studies of Staphylococcus aureus clinical isolated with monoclonal antibodies to capsular polysaccharide types 5 and 8. Journal of Clinical Microbiology 25:526–530
JanzonL.,
ArvidsonS.1990; The role of the δ-lysin gene (hid) in the regulation of virulence genes by the accessory gene regulator (agr) in Staphylococcus aureus
. EMBO Journal 9:1391–1399
JanzonL.,
LöfdahlS.,
ArvidsonS.1986; Evidence for a coordinate transcriptional control of alpha-toxin and protein A synthesis in Staphylococcus aureus
. FEMS Microbiology Letters 33:193–198
JassimS.,
SaltW. G.,
StrettonR. J.1989; In vitro studies of haemolysis by some staphylococci grown in chemically defined media. Journal of Applied Bacteriology 67:511–520
KarakawaW. W.,
FournierJ. M.,
VannW. F.,
ArbeitR.,
SchneersonR. S.,
RobbinsJ. B.1985; Method for the serological typing of the capsular polysaccharides of Staphylococcus aureus
. Journal of Clinical Microbiology 22:445–447
KarakawaW. W.,
SuttonA.,
SchneersonR. S.,
KarpasA.,
VannW. F.1988; Capsular antibodies induce type-specific phagocytosis of capsulated Staphylococcus aureus by human polymorphonuclear leukocytes. Infection and Immunity 56:1090–1095
KarakawaW. W.,
VannW. F.1982; Capsular polysaccharides of Staphylococcus aureus
. In Seminars in Infectious Disease: Bacterial Vaccines4 pp. 285–293RobbinsJ. B.,
HillJ. C.,
SadoffJ. C.
Edited by New York: Thieme Stratton;
LeeJ. C.,
LiuM. J.,
ParsonnetJ.,
ArbeitR. D.1990; Expression of type 8 capsular polysaccharide and production of toxic shock syndrome toxin 1 are associated among vaginal isolates of Staphylococcus aureus
. Journal of Clinical Microbiology 28:2612–2615
MarstonJ.,
FahlbergW. J.1960; Coagulase production by Staphylococcus aureus. II. Growth and coagulase production in complex and chemically defined mediums. Journal of Infectious Diseases 106:116–122
McdevittD.,
VaudauxP.,
FosterT. J.1992; Genetic evidence that bound coagulase of Staphylococcus aureus is not clumping factor. Infection and Immunity 60:1514–1523
MonodJ.,
PappenheimerA. M.JRCohen-BazireG.1952; La cinétique de la biosynthese de la β-galactosidase chez E. coli considerée comme fonction de la croissance. Biochimica et Biophysica Acta 9:648–660
O’reillyM.,
De AzavedoC. S.,
KennedyS.,
FosterT. J.1986; Inactivation of the alpha-haemolysin gene of Staphylococcus aureus 8325-4 by site-directed mutagenesis and studies on the expression of its haemolysins. Microbial Pathogenesis 1:125–138
PengH. L.,
NovickR. P.,
KreiswirthB.,
KornblumJ.,
SchlievertP.1988; Cloning, characterisation, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus
. Journal of Bacteriology 170:4365–4372
RecseiP.,
KreiswirthB.,
O’reillyM.,
SchlievertP.,
GrussA.,
NovickR. P.1986; Regulation of exoprotein gene expression in Staphylococcus aureus by agr
. Molecular and General Genetics 202:58–61
RegassaL. B.,
CouchJ. L.,
BetleyM. J.1991; Steady-state staphylococcal enterotoxin type C mRNA is affected by a product of the accessory gene regulatory (agr) and by glucose. Infection and Immunity 59:955–962
SompolinskyD.,
SamraZ.,
KarakawaW. W.,
VannW. F.,
SchneersonR.,
MalikZ.1985; Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. Journal of Clinical Microbiology 22:828–834
StringfellowW. T.,
DassyB.,
LiebM.,
FournierJ. M.1991; Staphylococcus aureus growth and type 5 capsular polysaccharide production in synthetic media. Applied and Environmental Microbiology 57:618–621
TangJ. L.,
GoughC. L.,
DanielsM. J.1990; Cloning of genes involved in negative regulation of production of extracellular enzymes and polysaccharide of Xanthomonas campestris pathovar campestris
. Molecular and General Genetics 222:157–160
TangJ. L.,
LiuY. N.,
BarberC. E.,
DowJ. M.,
WoottonJ. C.,
DanielsM. J.1991; Genetic and molecular analysis of a cluster of rpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris
. Molecular and General Genetics 226:409–417
VandeneschF.,
KornblumJ.,
NovickR. P.1991; A temporal signal independent of agr, is required for hla but not spa transcription in Staphylococcus aureus
. Journal of Bacteriology 173:6313–6320
WannerB. L.1992; Is cross regulation by phosphorylation of two- component response regulator proteins important in bacteria?. Journal of Bacteriology 174:2053–2058