1887

Abstract

A plasmid, designated pFS1, has been identified for the first time in a strain of . The plasmid is 16 kb in length. It is sensitive to digestion by DNAase I, restriction endonucleases and exonuclease III, but resistant to RNAase A and λ-exonuclease. A restriction map of the plasmid has been constructed, demonstrating its linear nature. The linear DNA molecules of pFS1 are attached at their 5′ ends to a protein which can be cleaved by Pronase. No homology was detected between pFS1 and chromosomal DNAs from a number of different strains. Also, hybridization between pFS1 and total DNA from SCE2 plasmid-cured derivatives does not occur. The plasmid seems to be cryptic since strains harbouring pFS1 and the cured derivatives have the same phenotype.

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1993-06-01
2021-10-20
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References

  1. Barbour A. G., Garon C. F. 1987; Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends. Science 237:409–411
    [Google Scholar]
  2. Bastos M. C. F., Penido E. G. C. 1981; Transduction in Staphylococcus aureus. II. Isolation of plasmids coding for drug resistance. Brazilian Journal of Genetics 4:41–53
    [Google Scholar]
  3. Claus D., Berkeley R. C. W. 1986; Genus Bacillus . In Bergey’s Manual of Systematic Bacteriology 2 pp. 1105–1139 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Davis R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics. A Manual for Genetic Engineering Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Debabov V. G. 1982; The industrial use of bacilli. In The Molecular Biology of the Bacilli I Bacillus subtilis pp. 331–370 Edited by Dubnau D. A. New York: Academic Press;
    [Google Scholar]
  6. Escarmis C., Salas M. 1981; Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage 𝜙29. Proceedings of the National Academy of Sciences of the United States of America 3:1446–1450
    [Google Scholar]
  7. Francou F. 1981; Isolation and characterization of a linear DNA molecule in the fungus Ascobolus immersus . Molecular and General Genetics 184:440–444
    [Google Scholar]
  8. Giambiagi-Marval M., Mafra M. A., Penido E. G. C., Bastos M. C. F. 1990; Distinct groups of plasmids correlated with bacteriocin production in Staphylococcus aureus . Journal of General Microbiology 136:1591–1599
    [Google Scholar]
  9. Grindley N. D. F., Reed R. R. 1985; Transpositional recombination in prokaryotes. Annual Review of Biochemistry 54:863–896
    [Google Scholar]
  10. Gryczan T. J. 1982; Molecular cloning in Bacillus subtilis . In The Molecular Biology of the Bacilli I Bacillus subtilis pp. 307–329 Edited by Dubnau D. A. New York: Academic Press;
    [Google Scholar]
  11. Gunge N. 1983; Yeast DNA plasmids. Annual Review of Microbiology 37:253–276
    [Google Scholar]
  12. Gunge N., Tamaru A., Ozawa F., Sakaguchi K. 1981; Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. Journal of Bacteriology 145:382–390
    [Google Scholar]
  13. Hashiba T., Homma Y., Hyakumachi M., Matsuda I. 1984; Isolation of a DNA plasmid in the fungus Rhizoctonia solani . Journal of General Microbiology 130:2067–2070
    [Google Scholar]
  14. Hayakawa T., Tanaka T., Sakaguchi K., Otake N., Yonehara H. 1979; A linear plasmid-like DNA in Streptomyces species producing lankacidin group antibiotics. Journal of General and Applied Microbiology 25:255–260
    [Google Scholar]
  15. Hirochika H., Sakaguchi K. 1982; Analysis of linear plasmids isolated from Streptomyces: association of protein with the ends of the plasmid DNA. Plasmid 7:59–65
    [Google Scholar]
  16. Hirochika H., Nakamura K., Sakaguchi K. 1984; A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs. EMBO Journal 3:761–766
    [Google Scholar]
  17. Kalkus J., Reh M., Schlegel H. G. 1990; Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids. Journal of General Microbiology 136:1145–1151
    [Google Scholar]
  18. Kempken F., Meinhardt F., Esser K. 1989; In organello replication and viral affinity of linear, extrachromosomal DNA of the ascomycete Ascobolus immersus . Molecular and General Genetics 218:523–530
    [Google Scholar]
  19. Kim W. K., Whitmore E., Klassen G. R. 1990; Homologous linear plasmids in mitochondria of three species of wheat bunt fungi, Tilletia caries, T. laevis and T. controversa . Current Genetics 17:229–233
    [Google Scholar]
  20. Kinashi H., Shimaji M., Sakai A. 1987; Giant linear plasmids in Streptomyces which code for antibiotics biosynthesis genes. Nature; London: 328454–455
    [Google Scholar]
  21. Kinashi H., Shimaji-Murayama M. 1991; Physical characterization of SCP1, a giant linear plasmid from Streptomyces coelicolor . Journal of Bacteriology 173:1523–1529
    [Google Scholar]
  22. Kitada K., Hishinuma F. 1987; A new linear DNA plasmid isolated from the yeast Saccharomyces kluyveri . Molecular and General Genetics 206:377–381
    [Google Scholar]
  23. Ligon J. M., Bolen P. L., Hill D. S., Bothast R. J., Kurtzman C. P. 1989; Physical and biological characterization of linear DNA plasmids of the yeast Pichia inositovora . Plasmid 21:185–194
    [Google Scholar]
  24. Line M. A., Louth M. W. 1971; Non-symbiotic nitrogen-fixing organisms from New Zealand tussock-grassland soils. Journal of General Microbiology 66:309–318
    [Google Scholar]
  25. Lovett P. S., Bramucci M. G. 1975; Plasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus . Journal of Bacteriology 124:484–490
    [Google Scholar]
  26. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Meinhardt F., Esser K. 1984; Linear extrachromosomal DNA in Morchella conica . Current Genetics 8:15–18
    [Google Scholar]
  28. Niwa O., Sakaguchi K., Gunge N. 1981; Curing of the killer deoxyribonucleic acid plasmids of Kluyveromyces lactis . Journal of Bacteriology 148:988–990
    [Google Scholar]
  29. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. 1979; Penicillinase plasmids of Staphylococcus aureus: restriction- deletion maps. Plasmid 2:109–129
    [Google Scholar]
  30. Palmer J. D., Shields C. R., Cohen D. B., Orton T. J. 1983; An unusual mitochondrial DNA plasmid in the genus Brassica . Nature; London: 301725–728
    [Google Scholar]
  31. Rosado A. S., Seldin L. 1993; Production of a potentially novel antimicrobial substance by Bacillus polymyxa . World Journal of Microbiology and Biotechnology (in the Press)
    [Google Scholar]
  32. Salas M., Mellado R. P., Vinuela E., Sogo J. M. 1978; Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage 𝜙29. Journal of Molecular Biology 119:269–291
    [Google Scholar]
  33. Sakaguchi K. 1990; Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adeno-type viruses. Microbiological Reviews 54:66–74
    [Google Scholar]
  34. Schleif R. F., Wensink P. C. 1981 Practical Methods in Molecular Biology New York: Springer-Verlag;
    [Google Scholar]
  35. Seldin L., Van Elsas J. D., Penido E. G. C. 1983; Bacillus nitrogen fixers from Brazilian soils. Plant and Soil 70:243–255
    [Google Scholar]
  36. Seldin L., Bastos M. C. F., Penido E. G. C. 1989; Identification of Bacillus azotofixans nitrogen fixation genes using heterologous nif probes. In Nitrogen Fixation with Non-legumes pp. 179–187 Edited by Skinner F. A., Boddey R. M., Fendrick I. Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  37. Tamanoi F., Stillman B. W. 1983; Initiation of adenovirus DNA replication in vitro requires a specific DNA sequence. Proceedings of the National Academy of Sciences of the United States of America 80:6446–6450
    [Google Scholar]
  38. Tanaka T., Koshikawa T. 1977; Isolation and characterization of four types of plasmids from Bacillus subtilis (natto). Journal of Bacteriology 131:699–701
    [Google Scholar]
  39. Tanaka T., Sakaguchi K. 1978; Construction of a recombinant plasmid composed of B. subtilis leucine genes and a B. subtilis (natto) plasmid: its use as cloning vehicle in B. subtilis 168. Molecular General Genetics 165:269–276
    [Google Scholar]
  40. Thuring R. W. J., Sanders J. P. M., Borst P. 1975; A freeze-squeeze method for recovering long DNA from agarose gels. Analytical Biochemistry 66:213–220
    [Google Scholar]
  41. Uozumi T., Ozaki A., Beppu T., Arima K. 1980; New cryptic plasmid of Bacillus subtilis and restriction analysis of other plasmids found by general screening. Journal of Bacteriology 142:315–318
    [Google Scholar]
  42. Yui Y., Katayose U., Shishido K. 1988; Two linear plasmid-like DNA elements simultaneously maintained in Pleurotus ostreatus . Biochimica et Biophysica Acta 951:53–60
    [Google Scholar]
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