1887

Abstract

Summary: The cryptic plasmid pCI305 from subsp. UC317 was screened for incompatibility with a range of cloning vectors and was found to be incompatible with the theta-replicating plasmids pAM401 and pIL253. In contrast, lactococcal vectors containing the replication functions of the rolling circle replicating plasmids pSH71 and pWV01 were compatible with pCI305. A number of native lactococcal plasmids were also screened for both incompatibility and DNA sequence homology to the pCI305 replicon. A plasmid with high sequence identity was identified which was compatible with pCI305.

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/content/journal/micro/10.1099/00221287-139-6-1271
1993-06-01
2019-10-19
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-6-1271
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