1887

Abstract

The methylenomycin biosynthetic gene cluster was previously shown to be present on the 350 kb giant linear plasmid SCP1. The structures of SCP1 in various A3(2) strains were analysed by pulsed field gel electrophoresis (PFGE). A3(2) strains 1147, M138 and M146, previously genetically characterized as SCP1, contained the free form of SCP1. 1984 and 2106, earlier characterized as SCP1′- and a stable donor respectively, were found to contain very large linear plasmids, 550 kb and 1700 kb in size. Strain JCM4979, another SCP1 derivative of A3(2), had a series of SCP1-related linear plasmids of 390630 kb. The integrated states of SCP1 in SCP1-NF strains, an NF-like strain and a stable donor were confirmed by PFGE, which revealed a lack of the intact SCP1 termini. In addition, a 70 kb linear plasmid was found in SANK95570, another methylenomycin producer. However, the plasmid was shown by hybridization to a methylenomycin resistance gene not to carry the methylenomycin gene cluster of this strain.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-6-1261
1993-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/6/mic-139-6-1261.html?itemId=/content/journal/micro/10.1099/00221287-139-6-1261&mimeType=html&fmt=ahah

References

  1. Aguilar A., Hopwood D. A. 1982; Determination of methylenomycin A synthesis by the pSV1 plasmid from Streptomyces violaceus-ruber SANK95570. Journal of General Microbiology 128:1893–1901
    [Google Scholar]
  2. Bibb M. J., Hopwood D. A. 1981; Genetic studies of the fertility plasmid SCP2 and its SCP2* variant in Streptomyces coelicolor A3(2). Journal of General Microbiology 126:427–442
    [Google Scholar]
  3. Bibb M., Schottel J. L., Cohen S. N. 1980; A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces . Nature; London: 284526–531
    [Google Scholar]
  4. Carle G. F., Olson M. V. 1987; Orthogonal-field-alternation gel electrophoresis. Methods in Enzymology 155:468–482
    [Google Scholar]
  5. Chater K. F., Bruton C. J. 1985; Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered. EMBO Journal 4:1893–1897
    [Google Scholar]
  6. Chater K. F., Henderson D. J., Bibb M. J., Hopwood D. A. 1988; Genome flux in Streptomyces coelicolor and other strepto- mycetes and its possible relevance to the evolution of mobile antibiotic resistance determinants. Symposia of the Society for General Microbiology 43:7–42
    [Google Scholar]
  7. Chu G., Vollrath D., Davis R. W. 1986; Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234:1582–1585
    [Google Scholar]
  8. Hanafusa T., Kinashi H. 1992; The structure of an integrated copy of the giant linear plasmid SCP1 in the chromosome of Streptomyces coelicolor 2612. Molecular and General Genetics 231:363–368
    [Google Scholar]
  9. Haneishi T., Kitahara N., Takiguchi Y., Arai M. 1974; New antibiotics, methylenomycins A and B. I. Producing organism, fermentation and isolation, biological activities and physical and chemical properties. Journal of Antibiotics 27:386–392
    [Google Scholar]
  10. Hopwood D. A. 1959; Linkage and the mechanism of recombination in Streptomyces coelicolor . Annals of the New York Academy of Sciences 81:887–898
    [Google Scholar]
  11. Hopwood D. A., Wright H. M. 1973; A plasmid of Streptomyces coelicolor carrying a chromosomal locus and its inter-specific transfer. Journal of General Microbiology 79:331–342
    [Google Scholar]
  12. Hopwood D. A., Wright H. M. 1976a; Genetic studies on SCP1-prime strains of Streptomyces coelicolor A3(2). Journal of General Microbiology 95:107–120
    [Google Scholar]
  13. Hopwood D. A., Wright H. M. 1976b; Interaction of the plasmid SCP1 with the chromosome of Streptomyces coelicolor A3(2). In Second International Symposium on the Genetics of Industrial Microorganisms pp. 607–619, Edited by. Macdonald K. D. London:: Academic Press.;
    [Google Scholar]
  14. Hopwood D. A., Harold R. J., Vivian A., Ferguson H. M. 1969; A new kind of fertility variant in Streptomyces coelicolor . Genetics 62:461–477
    [Google Scholar]
  15. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. 1973; Advances in Streptomyces coelicolor genetics. Bacteriological Reviews 37:371–405
    [Google Scholar]
  16. Hopwood D. A., Lydiate D. J., Malpartida F., Wright H. M. 1985a; Conjugative sex plasmids of Streptomyces . In Plasmids in Bacteria pp. 615–633, Edited by. Helinski D. R., Cohen S. N., Clewell D. B., Jackson D. A., Hollaender A. New York & London:: Plenum Press.;
    [Google Scholar]
  17. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. M., Ward J. M., Schrempf H. 1985b Genetic Manipulation of Streptomyces, a Laboratory Manual p. 257 Norwich:: John Innes Foundation.;
    [Google Scholar]
  18. Huetter R., Birch A., Haeusler A., Voegtli M., Madon J., Krek W. 1988; Genome fluidity in Streptomyces . In Biology of Actinomycetes ̓88 pp. 111–116, Edited by. Okami Y., Beppu T., Ogawara H. Tokyo:: Japan Scientific Societies Press.;
    [Google Scholar]
  19. Kendall K., Cullum J. 1986; Identification of a DNA sequence associated with plasmid integration in Streptomyces coelicolor A3(2). Molecular and General Genetics 202:240–245
    [Google Scholar]
  20. Kinashi H. 1989; Giant linear plasmids in various methylenomycin-producing strains of Streptomyces species. In Genetics and Molecular Biology of Industrial Microorganisms pp. 141–146, Edited by. Hershberger C. L., Queener S. W., Hegeman G. Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  21. Kinashi H., Shimaji-Murayama M. 1991; Physical characterization of SCP1, a giant linear plasmid from Streptomyces coelicolor . Journal of Bacteriology 173:1523–1529
    [Google Scholar]
  22. Kinashi H., Shimaji M., Sakai A. 1987; Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes. Nature; London: 328454–456
    [Google Scholar]
  23. Kinashi H., Shimaji-Murayama M., Hanafusa T. 1991; Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid, SCP1. Plasmid 26:123–130
    [Google Scholar]
  24. Kinashi H., Shimaji-Murayama M., Hanafusa T. 1992; Integration of SCP1, a giant linear plasmid, into the Streptomyces coelicolor chromosome. Gene 115:35–41
    [Google Scholar]
  25. Kirby R., Hopwood D. A. 1977; Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2). Journal of General Microbiology 98:239–252
    [Google Scholar]
  26. Kirby R., Wright L. F., Hopwood D. A. 1975; Plasmid-determined antibiotic synthesis and resistance in Streptomyces coelicolor . Nature; London: 254265–267
    [Google Scholar]
  27. Lydiate D. J., Malpartida F., Hopwood D. A. 1985; The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35:223–235
    [Google Scholar]
  28. Lydiate D. J., Ashby A. M., Henderson D. J., Kieser H. M., Hopwood D. A. 1989; Physical and genetic characterization of chromosomal copies of the Streptomyces coelicolor mini-circle. Journal of General Microbiology 135:941–955
    [Google Scholar]
  29. Mccormick J. R., Santamaria R., Losick R. 1991; The genes for three spore-associated proteins are encoded on linear plasmid SCP1 in Streptomyces coelicolor . Proceedings of the 8 th International Symposium on Biology of Actinomycetes, Madison, Wisconsin p. 125:
    [Google Scholar]
  30. Okanishi M., Manome T., Umezawa H. 1980; Isolation and characterization of plasmid DNAs in actinomycetes. Journal of Antibiotics 33:88–91
    [Google Scholar]
  31. Omer C. A., Cohen S. N. 1986; Structural analysis of plasmid and chromosomal loci involved in site-specific excision and integration of the SLP1 element of Streptomyces coelicolor . Journal of Bacteriology 166:999–1006
    [Google Scholar]
  32. Sakaguchi K. 1990; Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adeno-type viruses. Microbiological Reviews 54:66–74
    [Google Scholar]
  33. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning, a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory Press.;
    [Google Scholar]
  34. Schrempf H., Dyson P., Dittrich W., Betzler M., Habiger C., Mahro B., Bronneke V., Kessler A., Duvel H. 1988; Genetic instability in Streptomyces . In Biology of Actinomycetes ̓88 pp. 145–150, Edited by. Okami Y., Beppu T., Ogawara H. Tokyo:: Japan Scientific Societies Press.;
    [Google Scholar]
  35. Tanaka T., Kuroda M., Sakaguchi K. 1977; Isolation and characterization of four plasmids from Bacillus subtilis . Journal of Bacteriology 129:1487–1494
    [Google Scholar]
  36. Vivian A. 1971; Genetic control of fertility in Streptomyces coelicolor A3(2): Plasmid involvement in the interconversion of UF and IF strains. Journal of General Microbiology 69:353–364
    [Google Scholar]
  37. Vivian A., Hopwood D. A. 1970; Genetic control of fertility in Streptomyces coelicolor A3(2): the IF fertility type. Journal of General Microbiology 64:101–117
    [Google Scholar]
  38. Vivian A., Hopwood D. A. 1973; Genetic control of fertility in Streptomyces coelicolor A3(2): new kinds of donor strains. Journal of General Microbiology 76:147–162
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-6-1261
Loading
/content/journal/micro/10.1099/00221287-139-6-1261
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error