1887

Abstract

A cDNA library was constructed in λgt11 using mRNA from 11-d-old mycelium of . Three clones containing laccase sequence were identified using an affinity-purified anti-laccase antibody. From one of these clones, a 333 bp sequence was used to identify further cDNA clones (including one which is close to full length) and a genomic clone. The coding sequences found were of two similar but not identical versions with differences at 36 out of 520 residues of deduced amino acid sequence. The laccase genes each encode a sequence expressed as a 2·3 kb mRNA, specifying a 520 residue polypeptide including a 19 amino acid residue signal peptide that is absent from the N terminus of the mature (extracellular) protein. The coding sequence of 1 is interrupted by 14 short introns. The 1 and 2 genes are not allelic as they do not segregate in uninucleate spores derived from a four-spored basidium. Comparison of the deduced amino acid sequences with that of the other fungal laccases that have been cloned, and with the very similar ascorbate oxidases from higher plants shows that whilst some sequence is absolutely conserved at and around the amino acid residues involved in copper binding, the overall sequence similarities are low.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-6-1209
1993-06-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/6/mic-139-6-1209.html?itemId=/content/journal/micro/10.1099/00221287-139-6-1209&mimeType=html&fmt=ahah

References

  1. Aramayo R., Timberlake W. E. 1990; Sequence and molecular structure of the Aspergillus nidulans yA (laccase 1) gene. Nucleic Acids Research 18:3415
    [Google Scholar]
  2. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  3. Elliott T. J. 1972; Sex and the single spore. Mushroom Science 8:11–18
    [Google Scholar]
  4. Esser K., Minuth W. 1970; The phenoloxidases of the ascomycete Podospora anserina VI. Genetic regulation of the formation of laccase. Genetics 64:441–458
    [Google Scholar]
  5. Faisst S., Meyer S. 1992; Compilation of vertebrate-encoded transcription factors. Nucleic Acids Research 20:3–26
    [Google Scholar]
  6. Frischauf A. M., Lehrach H., Poutska A., Murray N. 1983; Lambda replacement vectors carrying poly linker sequences. Journal of Molecular Biology 170:827–842
    [Google Scholar]
  7. Germann U. A., Lerch K. 1986; Isolation and partial nucleotide sequence of the laccase genes from Neurospora crassa: amino acid sequence homology of the protein to human ceruloplasmin. Proceedings of the National Academy of Sciences of the United States of America 83:8854–8858
    [Google Scholar]
  8. Germann U. A., Muller G., Hunziker P. E., Lerch K. 1988; Characterisation of two allelic forms of Neurospora crassa laccase. Amino- and carboxyl-terminal processing of a precursor. Journal of Biological Chemistry 263:885–896
    [Google Scholar]
  9. Harmsen M. C., Tolner B., Kram A., Seung J. G., De Haan A., Wessels J. G. H. 1991; Sequences of three dsRNAs associated with La France disease of the cultivated mushroom (Agaricus bisporus). Current Genetics 20:137–144
    [Google Scholar]
  10. Harmsen M. C., Schuren F. H. J., Moukha S. M., Van Zuilen C. M., Punt P. J., Wessels J. G. H. 1992; Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from Schizophyllum commune, Phanerochaete chrysosporium and Agaricus bisporus . Current Genetics 22:447–454
    [Google Scholar]
  11. Huynh T. V., Young R. A., Davis R. W. 1983; Construction and screening of cDNA libraries in λgtl0 and λgtll. In DNA Cloning. A Practical Approach pp 49–78 Edited by Glover D. M. Oxford: IRL Press;
    [Google Scholar]
  12. Katagiri F., Chua N.-H. 1992; Plant transcription factors: present knowledge and future challenges. Trends in Genetics 8:22–27
    [Google Scholar]
  13. Kojima Y., Tsukuda Y., Kawai Y., Tsukamoto A., Sugiura J., Sakaimo M., Kita Y. 1990; Cloning, sequence analysis, and expression of lignolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus . Journal of Biological Chemistry 265:15224–15230
    [Google Scholar]
  14. Loftus M. G., Moore D., Elliott T. J. 1988; DNA polymorphism in commercial and wild strains of the cultivated mushroom, Agaricus bisporus . Theoretical and Applied Genetics 76:712–718
    [Google Scholar]
  15. Mcbride N. K., Thurston C. F. 1983; The complexity of Poly(A)+ RNA in the alga Chlorella fusca . Journal of Cell Science 63:223–231
    [Google Scholar]
  16. Marbach I., Harel E., Mayer A. L. 1984; Molecular properties of extracellular Botrytis cinerea laccase. Phytochemistry 23:2713–2717
    [Google Scholar]
  17. Messerschmidt A., Huber R. 1990; The blue oxidases, ascorbate oxidase, laccase and ceruloplasmin. Modelling and structural relationships. European Journal of Biochemistry 187:341–352
    [Google Scholar]
  18. Messerschmidt A., Rossi A., Ladenstein R., Huber R., Bolognesi M., Gatti G., Marchesini A., Petruzelli R., Finazzi-Agro A. 1989; X-ray crystal structure of the blue oxidase ascorbate oxidase from Zucchini. Analysis of the polypeptide fold and a model of the copper sites and ligands. Journal of Molecular Biology 206:513–529
    [Google Scholar]
  19. Ohkawa J., Okada N., Shinmyo A., Takano M. 1989; Primary structure of cucumber (Cucumis sativus) ascorbate oxidase deduced from cDNA sequence: homology with blue copper proteins and tissue-specific expression. Proceedings of the National Academy of Sciences of the United States of America 861239–1243
    [Google Scholar]
  20. Perry C. R., Matcham S. E., Wood D. A., Thurston C. F. 1993; The structure of laccase protein and its synthesis by the commercial mushroom Agaricus bisporus . Journal of General Microbiology 139:171–178
    [Google Scholar]
  21. Pollard J. W., Perry C. R., Thurston C. F. 1988; Northern blotting. In Methods of Molecular Biology, 3 pp. 13–26 Edited by Walker J. Clifton: Humana Press Inc.;
    [Google Scholar]
  22. Raguz S., Yague E., Wood D. A., Thurston C. F. 1992; Isolation and characterisation of a cellulose-growth-specific gene from Agaricus bisporus . Gene 119:183–190
    [Google Scholar]
  23. Rehman A. U., Thurston C. F. 1992; Purification of laccase I from Armillaria mellea . Journal of General Microbiology 138:1251–1257
    [Google Scholar]
  24. Saloheimo M., Niku-paavola M.-L., Knowles J. K. C. 1991; Isolation and structural analysis of the laccase gene from the lignindegrading fungus Phlebia radiata . Journal of General Microbiology 137:1537–1544
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning. A Laboratory Manual, 2nd. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Sanger F., Nicklen S., Coulson A. R. 1977; DNA Sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  27. Smith J. F., Claydon N., Love M. E., Allan M., Wood D. A. 1989; Effect of substrate depth on extracellular endocellulase and laccase production of Agaricus bisporus . Mycological Research 93:292–296
    [Google Scholar]
  28. Sodhi H. S. 1992 Enzyme mutants of Coprinus bilanatus and recombinant DNA technology for strain improvement in Agaricus bisporus PhD. thesis, University of London
    [Google Scholar]
  29. Thurston C. F., Perry C. R., Pollard J. W. 1988; Electrophoresis of RNA denatured with glyoxal or formaldehyde. In Methods of Molecular Biology 3 pp. 3–12 Edited by Walker J. Clifton: Humana Press Inc;
    [Google Scholar]
  30. Wood D. A. 1980a; Production, purification and properties of extracellular laccase of Agaricus bisporus . Journal of General Microbiology 117:327–338
    [Google Scholar]
  31. Wood D. A. 1980b; Inactivation of extracellular laccase during fruiting of Agaricus bisporus . Journal of General Microbiology 117:339–345
    [Google Scholar]
  32. Wood D. A., Goodenough P. W. 1977; Fruiting of Agaricus bisporus. Changes in extracellular enzyme activities during growth and fruiting. Archives of Microbiology 114:161–165
    [Google Scholar]
  33. Young R. A., Davis R. W. 1983; Yeast polymerase II genes: isolation with antibody probes. Science 222:778–782
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-6-1209
Loading
/content/journal/micro/10.1099/00221287-139-6-1209
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error