1887

Abstract

strain 81116 contains two flagellin genes, and . Wild-type (WT) bacteria express only, but can be expressed under certain conditions. We have determined the importance of flagella for colonization of the avian caecum, which appears to be the natural environment for these bacteria. Mutants in which or , or both had been inactivated, and motility variants, were investigated. Flagella are not a requisite for colonization, but mutants lacking both flagellin genes colonized less efficiently than WT. Inactivation of the gene, which had no effect on bacterial motility, enhanced chicken caecal colonization 1000-fold compared to WT. A variant (SF-1) with flagella composed of flagellin A, but with poor motility, also colonized better than WT. Conversely, mutants with an inactivated gene colonized 100- to 1000-fold less efficiently than WT, regardless of their motility conferred by truncated or full-length flagellin B flagella. These results suggest that the presence of flagellin A, rather than motility, is essential for optimal bacterial colonization of chicken caeca.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-6-1171
1993-06-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/6/mic-139-6-1171.html?itemId=/content/journal/micro/10.1099/00221287-139-6-1171&mimeType=html&fmt=ahah

References

  1. Aguero-Rosenfeld M. E., Yang X. H., Nachamkin I. 1990; Infection of Syrian hamsters with flagellar variants of Campylobacter jejuni . Infection and Immunity 58:2214–2219
    [Google Scholar]
  2. Black R. E., Levine M. M., Clements M. L., Hughes T. P., Blaser M. J. 1988; Experimental Campylobacter jejuni infection in humans. Journal of Infectious Diseases 157:472–479
    [Google Scholar]
  3. Blaser J. M., Reller L. B. 1981; Campylobacter enteritis. New England Journal of Medicine 305:1444–1452
    [Google Scholar]
  4. Caldwell M. B., Guerry P., Lee E. C., Burans J. P., Walker R. I. 1985; Reversible expression of flagella in Campylobacter jejuni . Infection and Immunity 50:941–943
    [Google Scholar]
  5. De Melo M. A., Pechère J.-C. 1990; Identification of Campylobacter jejuni surface proteins that bind to eucaryotic cells in vitro . Infection and Immunity 58:1749–1756
    [Google Scholar]
  6. Guerrant R. L., Wanke C. A., Pennie R. A., Barrett L. J., Lima A. A. M., O’Brien A. D. 1987; Production of a unique cytotoxin by Campylobacter jejuni . Infection and Immunity 55:2526–2530
    [Google Scholar]
  7. Guerry P., Logan S. M., Thornton S., Trust T. J. 1990; Genomic organization and expression of Campylobacter flagellin genes. Journal of Bacteriology 172:1853–1860
    [Google Scholar]
  8. Guerry P., Alm R. A., Power M. E., Logan S. M., Trust T. J. 1991; Role of two flagellin genes in Campylobacter motility. Journal of Bacteriology 173:4757–4764
    [Google Scholar]
  9. Harris N. V., Weiss N. S., Nolan C. M. 1986; The role of poultry and meats in the etiology of Campylobacter jejuni/coli enteritis. American Journal of Public Health 76:407–411
    [Google Scholar]
  10. Mahajan S., Rodgers F. G. 1990; Isolation, characterization and host-cell-binding properties of a cytotoxin from Campylobacter jejuni . Journal of Clinical Microbiology 28:1314–1320
    [Google Scholar]
  11. Meinersmann R. J., Stern N. J., Blankenship L. C. 1990; Antigenic differences in congenic chicken-colonizing and noncolonizing strains of Campylobacter jejuni . Current Microbiology 21:17–21
    [Google Scholar]
  12. Morooka T., Umeka A., Amako K. 1985; Motility as an intestinal colonization factor for Campylobacter jejuni . Journal of General Microbiology 131:1973–1980
    [Google Scholar]
  13. Lee A., O’Rourke J., Barrington P. J., Trust T. J. 1986; Mucus colonization as a determinant of pathogenicity in intestinal infection by Campylobacter jejuni: a mouse caecal model. Infection and Immunity 51:5536–546
    [Google Scholar]
  14. Newell D. G., Mcbride H., Pearson A. D. 1984; The identification of outer membrane proteins and flagella of Campylobacter jejuni . Journal of General Microbiology 130:1201–1208
    [Google Scholar]
  15. Newell D. G., Mcbride G., Dolby J. M. 1985; Investigations on the role of flagella in the colonization of infant mice with Campylobacter jejuni and attachment of Campylobacter jejuni to human epithelial cell lines. Journal of Hygiene 95:217–227
    [Google Scholar]
  16. Nuijten P. J. M., Bleumink-Pluym N. M. C., Gaastra W., Van Der Zeijst B. A. M. 1989; Flagellin expression in Campylobacter jejuni is regulated at the transcriptional level. Infection and Immunity 57:1084–1088
    [Google Scholar]
  17. Nuijten P. J. M., Van Asten F. J. A. M., Gaastra W., Van Der Zeijst B. A. M. 1990; Structural and functional analysis of two Campylobacter jejuni flagellin genes. Journal of Biological Chemistry 265:17798–17804
    [Google Scholar]
  18. Nuijten P. J. M., Van Der Zeijst B. A. M., Newell D. G. 1991; Localization of immunogenic regions on the flagellin proteins of Campylobacter jejuni 81116 . Infection and Immunity 59:1100–1105
    [Google Scholar]
  19. Palmer S. R., Gully P. R., White J. M., Pearson A. D., Suckling W. G., Jones D. M., Rawes J. C. L., Penner J. L. 1983; Waterborne outbreak of Campylobacter gastroenteritis. Lancet i:287–290
    [Google Scholar]
  20. Pavlovskis O. R., Rollins D. M., Haberberger R. L., Green A. E., Habash L., Strocko S., Walker R. I. 1991; Significance of flagella in colonization resistance of rabbits immunized with Campylobacter spp. Infection and Immunity 59:2259–2264
    [Google Scholar]
  21. Shane S. M. 1992; The significance of Campylobacter jejuni infection in poultry: a review. Avian Pathology 21:189–213
    [Google Scholar]
  22. Skirrows M. B. 1977; Campylobacter enteritis - a new disease. British Medical Journal 2:9–11
    [Google Scholar]
  23. Stern N. J., Bailey J. S., Blankenship L. C., Cox N. A., Mchan F. 1988; Colonization characteristics of Campylobacter jejuni in chick caeca. Avian Diseases 32:330–334
    [Google Scholar]
  24. Wassenaar T. M., Bleumink-Pluym N. M. C., Van Der Zeijst B.A. M. 1991; Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion. EMBO Journal 10:2055–2061
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-6-1171
Loading
/content/journal/micro/10.1099/00221287-139-6-1171
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error