1887

Abstract

We investigated the usefulness of rRNA gene restriction fragment length polymorphism (RFLP) for grouping of the isolates associated with Lyme disease or from ixodid ticks. Genomic DNA was digested with a restriction enzyme, blotted and hybridized with an (23S rRNA) gene probe. The sizes of the restriction bands showed a good correlation with the genotypes recently proposed, and isolates of diverse geographic origin formed four distinct DNA groups. Group I included all of the USA isolates and some European isolates; group II contained European isolates and Asian isolates; group III comprised European and Asian isolates; group IV included Japanese isolates and an eastern Russian isolate. Groups I, II and III corresponded to and group VS461, respectively. The RFLPs of Japanese isolates were rather divergent and some of the isolates were quite distinct from the USA and European isolates. RFLP analysis of the rRNA genes and flanking regions, using gene probes as we reported here, may be useful in the taxonomic study of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-6-1141
1993-06-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/6/mic-139-6-1141.html?itemId=/content/journal/micro/10.1099/00221287-139-6-1141&mimeType=html&fmt=ahah

References

  1. Adam T., Gassman G. S., Rasiah C., Göbel U. B. 1991; Phenotypic and genotypic analysis of B. burgdorferi isolates from various sources. Infection and Immunity 59:2579–2585
    [Google Scholar]
  2. Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J. C., Assous M., Grimont P. A. D. 1992; Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. International Journal of Systematic Bacteriology 42:378–383
    [Google Scholar]
  3. Barbour A. G. 1984; Isolation and cultivation of Lyme disease spirochetes. Yale Journal of Biology and Medicine 57:521–525
    [Google Scholar]
  4. Barbour A. G., Garon C. F. 1987; Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends. Science 237:409–411
    [Google Scholar]
  5. Barbour A. G., Hayes S. F. 1986; Biology of Borrelia species. Microbiological Reviews 50:381–400
    [Google Scholar]
  6. Barbour A. G., Heiland R. A., Howe T. R. 1985; Heterogeneity of major proteins in Lyme disease Borrelia: a molecular analysis of North American and European isolates. Journal of Infectious Diseases 152:478–484
    [Google Scholar]
  7. Bergström S., Bundoc V. G., Barbour A. G. 1989; Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochete Borrelia burgdorferi . Molecular Microbiology 3:478–486
    [Google Scholar]
  8. Burgdorfer W., Barbour A. G., Hayes S. F., Benach J. L., Grunwaldt E., Davis J. P. 1982; Lyme disease - a tick borne spirochetosis?. Sciences 216:1317–1319
    [Google Scholar]
  9. Davidson B. E., Macdougall J., Saint Girons I. 1992; Physical maps of the linear chromosome of the bacterium Borrelia burgdorferi 212, a causative agent of Lyme disease, and localization of rRNA genes. Journal of Bacteriology 174:3766–3774
    [Google Scholar]
  10. Fukunaga M., Mifuchi I. 1989a; The number of large ribosomal RNA genes in Leptospira interrogans and Leptospira biflexa . Microbiology and Immunology 33:459–466
    [Google Scholar]
  11. Fukunaga M., Mifuchi I. 1989b; Unique organization of Leptospira interrogans rRNA genes. Journal of Bacteriology 171:5763–5767
    [Google Scholar]
  12. Fukunaga M., Sohnaka M. 1992; Tandem repeat of the 23S and 5S ribosomal RNA genes in Borrelia burgdorferi, the etiological agent of Lyme disease. Biochemical and Biophysical Research Communications 183:952–957
    [Google Scholar]
  13. Fukunaga M., Yanagihara Y., Sohnaka M. 1992; The 23S/5S ribosomal RNA genes (rrl/rrf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease. Journal of General Microbiology 138:871–877
    [Google Scholar]
  14. Grimont F., Grimont P. A. D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Annales de l’Institut Pasteur/Microbiology 137B:165–175
    [Google Scholar]
  15. Howe T. R., Mayer L. W., Barbour A. G. 1985; A single recombination plasmid expressing two major surface proteins of the Lyme disease spirochete. Science 227:645–646
    [Google Scholar]
  16. Hughes C. A. N., Johnson R. C. 1990; Methylated DNA in Borrelia species. Journal of Bacteriology 172:6602–6604
    [Google Scholar]
  17. Hughes C. A. N., Kodner C. B., Johnson R. C. 1992; DNA analysis of Borrelia burgdorferi NCH-1, the first northcentral U. S. human Lyme disease isolate. Journal of Clinical Microbiology 30:698–703
    [Google Scholar]
  18. Hyde F. W., Johnson R. C. 1984; Genetic relationship of Lyme disease spirochetes to Borrelia, Treponema and Leptospira . Journal of Clinical Microbiology 20:151–154
    [Google Scholar]
  19. Johnson R. C., Hyde F. W., Schmidt G. P., Brenner D. J. 1984; Borrelia burgdorferi sp. nov.: etiological agent of Lyme disease. International Journal of Systematic Bacteriology 34:496–497
    [Google Scholar]
  20. Lefebvre R. B., Perng G. C., Johnson R. C. 1989; Characterization of Borrelia burgdorferi isolates by restriction endonuclease analysis and DNA hybridization. Journal of Clinical Microbiology 27:636–639
    [Google Scholar]
  21. Luccini G. M., Altwegg M. 1992; rRNA gene restriction patterns as taxonomic tools for the genus Aeromonas . International Journal of Systematic Bacteriology 42:384–389
    [Google Scholar]
  22. Nakao M., Miyamoto K., Uchikawa K., Fujita H. 1992; Characterization of Borrelia burgdorferi isolated from Ixodes persulcatus and Ixodes ovatus ticks in Japan. American Journal of Tropical Medicine and Hygiene 47:505–511
    [Google Scholar]
  23. Nomura M., Gourse R., Baughman G. 1984; Regulation of the synthesis of ribosomes and ribosomal components. Annual Review of Biochemistry 53:75–117
    [Google Scholar]
  24. Nomura M., Morgan E. A. 1977; Genetics of bacterial ribosomes. Annual Review of Genetics 11:297–347
    [Google Scholar]
  25. Postic D., Edlinger C., Richaud C., Grimont F., Dufresne Y., Perolat P., Baranton G., Grimont P. A. D. 1990; Two genomic species in Borrelia burgdorferi . Research in Microbiology 141:465–475
    [Google Scholar]
  26. Schwan T. G., Burgdorfer W., Schrumpf M. E., Karstens R. H. 1988; The urinary bladder, a consistent source of Borrelia burgdorferi in experimentally infected white-footed mice (Peromyscus leucopus). Journal of Clinical Microbiology 26:893–895
    [Google Scholar]
  27. Schwartz J. J., Gazumyan A., Schwartz I. 1992; rRNA gene organization in the Lyme disease spirochete, Borrelia burgdorferi . Journal of Bacteriology 174:3757–3765
    [Google Scholar]
  28. Smith C. J., Callihan D. R. 1992; Analysis of rRNA restriction fragment length polymorphism from Bacteroides spp. and Bacteroides fragilis isolates associated with diarrhea in humans and animals. Journal of Clinical Microbiology 30:806–812
    [Google Scholar]
  29. Srivastava A. K., Schlessinger D. 1990; Mechanism and regulation of bacterial ribosomal RNA processing. Annual Review of Microbiology 44:105–129
    [Google Scholar]
  30. Stull T. L., Lipuma J. J., Edlind T. D. 1988; A broad spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. Journal of Infectious Diseases 157:280–286
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-6-1141
Loading
/content/journal/micro/10.1099/00221287-139-6-1141
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error