Summary: Molecular fingerprints of chromosomal genotypes in were generated by analysis of variation at the 16S gene loci and the sites of the insertion sequence IS. Genetic and reference strains of were compared with clinical phage type strains from cases of human salmonellosis. Three 16S profiles, one of which was predominant, were found. The copy number of the -specific insertion sequence IS varied from 6 to 12, and all insertions were chromosomal. Three of the insertion sites shared by all strains were serovar-specific for Thirteen distinct profiles of IS were detected, providing a high level of intraserovar strain discrimination. Profiles were generally more conserved among genetic and reference strains; representatives of clinical phage type strains, which are recent human isolates, showed much greater diversity of IS profiles. Irrespective of their origin, strains could be assigned to IS profile groups, phylogenetically related lines identified by combinations of conserved insertion sites. Hybridization profiles of this mobile element are markers of intermediate and short-term evolution in They provide a fingerprinting scheme for the purposes of genetics, and delineate a molecular typing scheme for the purposes of epidemiology.


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