Protein secretion and growth were investigated in Phanerochaete chrysosporium by using cultures sandwiched between perforated polycarbonate membranes. Labelling of colonies with radioactive N-acetylglucosamine and l-methionine indicated a close correlation between growth and general protein secretion, even in a central area of the colony secreting the idiophase enzymes lignin peroxidase (LiP) and manganese-dependent lignin peroxidase (MnP). Comparison of the sites of release into the medium of newly synthesized proteins and immuno-detected lignin peroxidases suggested that diffusion of the enzymes from the walls was a limiting step in the release of peroxidases into the medium. Microautoradiography of colonies exposed to N-acetyl[3H]glucosamine revealed the apical growth of thin hyphae and branches (4 to 5 μm diameter on average) in the central secreting area. These secondary hyphae showed peroxidase activity and reacted with lignin peroxidase antibodies. Although it was not possible to directly visualize secretion at hyphal tips, the results suggest that peroxidases (LiP and MnP) are initially secreted at the apex of secondary growing hyphae and later slowly released into the surrounding medium.
AndrawisA.,
PeaseE. A.,
KuanI. -C.,
HolzbaurE.,
TienM.1989; Characterization of two lignin peroxidase cDNA clones from Phanerochaete chrysosporium. Biochemical and Biophysical Research communications 162:673–680
AstherM.,
LesageL.,
DraponR.,
CorrieuG.,
OdierE.1988; Phospholipid and fatty acid enrichment of Phanerochaete chrysosporium INA-12 in relation to ligninase production. Applied Microbiology and Biotechnology 27:393–398
BlanchetteR. A.,
AbadA. R.,
FarrellR. L.,
LeathersT. D.1989; Detection of lignin peroxidase and xylanase by immunocytochemical labeling in wood decayed by basidiomycetes. Applied and Environmental Microbiology 55:1457–1465
BonnarmsP.,
JeffriesT. W.1990; Mn(II) regulation of lignin peroxidases and manganese-dependent peroxidases from lignindegrading white rot fungi. Applied and Enuironmenial Microbiology 56:210–217
CapdevilaC.,
MoukhaS.,
GhyczyM.,
TheilleuxJ.,
GelieB.,
DelattreM.,
CorrieuG.,
AstherM.1990; Characterization of peroxidase secretion and subcellularorganization of Phanerochaete chrysosporium INA-12 in the presence of various soybean phospholipid fractions. Applied and Environmental Microbiology 56:3811–3816
DanielG.,
NilssonT.,
PettersonB.1989; Intra- and extracellular localization of lignin peroxidase during the degradation of solid wood and wood fragments by Phanerochaete chrysosporium by using transmission electron microscopy and immunogold labelling. Applied and Environmental Microbiology 55:871–881
DanielG.,
PettersonB.,
NilssonT.,
VolcJ.1990; Use of immunogold cytochemistry to detect Mn(II)-dependent and lignin peroxidases in wood degraded by the white rot fungi Phanerochaete chrysosparium and Lentinula edodes. Canadian Journal of Botany 68:920–933
DattaA.,
BettermannA.,
KirkT. K.1991; Identification of a specific manganese peroxidase among ligninolytic enzymes secreted by Phanerochaete chrysosporium during wood decay. Applied and Environmental Microbiology 57:1453–1460
De BoerH. A.,
ZhangY. Z.,
CollinsC.,
ReddyC. A.1987; Analysis of nucleotide sequences of two ligninase cDNAs from a white-rot filamentous fungus,Phanerochaete chrysosporium. Gene 60:93–102
FaisonB. D.,
KirkT. K.1985; Factors involved in the regulation of a ligninase activity in Phanerochaete chrysosporium. Applied and Environmental Microbiology 49:299–304
ForneyL. J.,
ReddyC. A.,
PankratzH. S.1982; Ultrastructural localization of hydrogen peroxide production in ligninolytic Phanerochaete chrysosporium cells. Applied and Environmental Microbiology 44:732–736
GlennJ. K.,
GoldM. H.1983; Decolorization of several polymeric dyes by the lignin-degrading basidiomycete Phanerochaete chrysosporium. Applied and Environmental Microbialogy 45:1741–1747
GlennJ. K.,
GoldM. H.1985; Purification and characterization of an extracellular Mn(II)-dependent peroxidase from the lignin-degrading basidiomycete,Phanerochaete chrysosporium. Archives of Biochemistry and Biophysics 242:329–341
GlennJ. K.,
MorganM. A.,
MayfieldM. B.,
KuwaharaM.,
GoldM. H.1983; An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete Phanerochaete chrysosporium. Biochemical and Biophysical Research Communications 114:1077–1083
HolzbaurE. L. F.,
AndrawisA.,
TienM.1989; Structure and regulation of a lignin peroxidase gene from Phanerochaete chrysosporium. Biochemical and Biophysical Research Communications 155:626–633
JeffriesT. W.,
ChoiS.,
KirkT. K.1981; Nutritional regulation of lignin degradation by Phanerochaete chrysosporium. Applied and Environmental Microbiology 42:296–296
KeyserP.,
KirkT. K.,
ZeikusJ. G.1978; Ligninolytic enzyme system of Phanerochaete chrysosporium:synthesized in the absence of lignin in response to nitrogen starvation. Journal of Bacteriology 135:796–797
KirkT. K.,
SchutzE.,
ConnorsW. J.,
LorenzL. F.,
ZeikusJ. G.1978; Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Archives of Microbiology 117:277–285
KirkT. K.,
CroanS.,
TienK.,
MurtaghK. E.,
FarrellR. L.1986; Production of multiple ligninases by Phanerochaete chrysosporium: effect of selected growth conditions and use of a mutant strain. Enzyme and Microbial Technology 8:27–32
KuwaharaM.,
GlennJ. K.,
MorganM. A.,
GoldM. H.1984; Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Letters 169:247–250
LacknerR.,
SrebotnikE.,
MessnerK.1991; Immunogold-silver staining of extracellular ligninases secreted by Phanerochaete chrysosporium. Canadian Journal of Microbiology 37:665–668
LeisolaM. S. A.,
KozulicB.,
MeussdoerfferF.,
FiechterA.1987; Homology among multiple extracellular peroxidases from Phanerochaete chrysosporium. Journal of Biological Chemistry 262:419–424
MesulamM.-M.1982; Principles of horseradish peroxidase neurohistochemistry and their applications for tracing neural path-ways-axonal transport, enzyme histochemistry and light microscopic analysis. In Tracing Neural Connections with Horseradish Peroxidase pp. 1–151, Edited by.
MesulamM. -M.
London: Wiley.;
OrthA. B.,
DennyM.,
TienM.1991; Overproduction of lignin-degrading enzymes by an isolate of Phanerochaete chrysosporium. Applied and Environmental Microbiology 57:2591–2596
PribnowD.,
MayfieldM. B.,
NipperV. J.,
BrownJ. A.,
GoldM. H.1989; Characterization of cDNA encoding a manganese peroxidase, from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Journal of Biological Chemistry 264:5036–5040
RitchT. G.,
NipperV. J.,
AkileswaranL.,
SmithA. J.,
PribnowD. G.,
GoldM. H.1991; Lignin peroxidase from the basidiomycete Phanerochaete chrysosporium is synthesized as a preproenzyme. Gene 107:119–126
RochP.,
BuswellJ. A.,
CainB. R.,
OdierE.1989; Lignin peroxidase production by strains of Phanerochaete chrysosporium grown on glycerol. Applied Microbiology and Biotechnology 31:587–591
RuelK.,
JoseleauJ. -P.1991; Involvement of an extracellular glucan sheath during degradation of Populus wood by Phanerochaete chrysosporium. Applied and Environmental Microbiology 57:374–384
SrebotnikE.,
MessierK.,
FoisnerR.,
PettersonB.1988; Ultrastructural localization of ligninase of Phanerochaete chrysosporium by immunogold labeling. Current Microbiology 16:221–227
TrevithickJ. R.,
MetzenbergR. L.1966; Genetic alteration of pore size and other properties of the Neurospora cell wall. Journal of Bacteriology 92:1016–1020
UlmerD. C.,
LeisolaM. S. A.,
FiechterA.1984; Possible induction of the ligninolytic system of Phanerochaete chrysosporium. Journal of Biotechnology 1:13–14
VesselsJ. G. H.1990; Role of cell architecture in fungal tip growth generation. In Tip Growth in Plant and Fungal Walls pp. 1–29HeathI. B.
Edited by San Diego: Academic Press.;
WöstenH. A. B.,
MoukhaS. M.,
SietsmaJ. H.,
WesselsJ. G. H.1991; Localization of growth and secretion of proteins in Aspergillus niger. Journal of General Microbiology 137:2017–2023