%0 Journal Article %A Gupta, Anil K. %A Gautam, Sant P. %T Pyrification and properties of an extracellular α-glucosidase from the thermophilic fungus Malbranchea sulfurea %D 1993 %J Microbiology, %V 139 %N 5 %P 963-967 %@ 1465-2080 %R https://doi.org/10.1099/00221287-139-5-963 %I Microbiology Society, %X Summary: An extracellular α-glucosidase from the thermophilic fungus Malbranchea sulfurea was purified 31.6-fold with a yield of 11.68%. The size of the enzyme was 110 kDa. The optimum pH and temperature for the enzyme activity were pH 4.8 and 60°C, respectively; the half-life of the activity was 45 min at 65°C, 125 min at 60°C, and 6 h at 55°C. The substrate preference of the α-glucosidase was p-nitrophenyl-α-D-glucoside > sucrose > maltose > maltotriose. No activity was recorded against isomaltose, methyl-α-D-glucoside and starch. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-5-963