1887

Abstract

Summary: An extracellular α-glucosidase from the thermophilic fungus was purified 31.6-fold with a yield of 11.68%. The size of the enzyme was 110 kDa. The optimum pH and temperature for the enzyme activity were pH 4.8 and 60°C, respectively; the half-life of the activity was 45 min at 65°C, 125 min at 60°C, and 6 h at 55°C. The substrate preference of the α-glucosidase was -nitrophenyl-α-D-glucoside > sucrose > maltose > maltotriose. No activity was recorded against isomaltose, methyl-α-D-glucoside and starch.

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/content/journal/micro/10.1099/00221287-139-5-963
1993-05-01
2021-05-18
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