RT Journal Article SR Electronic(1) A1 Sato, Yutaka A1 Yamamoto, Yasuhito A1 Kizaki, Harutoshi A1 Kuramitsu, Howard K.YR 1993 T1 Isolation, characterization and sequence analysis of the scrK gene encoding fructokinase of Streptococcus mutans JF Microbiology, VO 139 IS 5 SP 921 OP 927 DO https://doi.org/10.1099/00221287-139-5-921 PB Microbiology Society, SN 1465-2080, AB A gene encoding an ATP-dependent fructokinase from Streptococcus mutans GS-5 was identified within a 2 kb DNA fragment immediately downstream from the scrA gene.The gene cloned in Escherichia coli also expressed mannokinase activity. Insertional inactivation of this gene in S. mutans markedly decreased both fructokinase and mannokinase activities. Nucleotide sequence analysis of the 2 kb fragment revealed an ORF starting 199 bp downstream from the scr A gene, preceded by potential ribosome-binding (Shine-Dalgarno) and promoter-like sequences. This ORF specified a putative protein of 293 amino acids with a calculated M r of 31681. The deduced amino acid sequence of the fructokinase gene, scrK, from S. mutans exhibited no significant similarity to fructokinase genes from Klebsiella pneumoniae, E. coli plasmid pUR400 or Vibrio alginolyticus, but was similar to a comparable gene from Zymomonas mobilis. , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-5-921