Summary: A gene encoding an ATP-dependent fructokinase from GS-5 was identified within a 2 kb DNA fragment immediately downstream from the gene. The gene cloned in also expressed mannokinase activity. Insertional inactivation of this gene in markedly decreased both fructokinase and mannokinase activities. Nucleotide sequence analysis of the 2 kb fragment revealed an ORF starting 199 bp downstream from the gene, preceded by potential ribosome-binding (Shine-Dalgarno) and promoter-like sequences. This ORF specified a putative protein of 293 amino acids with a calculated of 31681. The deduced amino acid sequence of the fructokinase gene, , from exhibited no significant similarity to fructokinase genes from plasmid pUR400 or , but was similar to a comparable gene from


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