1887

Abstract

A gene encoding an ATP-dependent fructokinase from GS-5 was identified within a 2 kb DNA fragment immediately downstream from the gene.The gene cloned in also expressed mannokinase activity. Insertional inactivation of this gene in markedly decreased both fructokinase and mannokinase activities. Nucleotide sequence analysis of the 2 kb fragment revealed an ORF starting 199 bp downstream from the gene, preceded by potential ribosome-binding (Shine-Dalgarno) and promoter-like sequences. This ORF specified a putative protein of 293 amino acids with a calculated of 31681. The deduced amino acid sequence of the fructokinase gene, , from exhibited no significant similarity to fructokinase genes from plasmid pUR400 or , but was similar to a comparable gene from

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-5-921
1993-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/5/mic-139-5-921.html?itemId=/content/journal/micro/10.1099/00221287-139-5-921&mimeType=html&fmt=ahah

References

  1. Aulkemeyer P., Ebner R., Heilenmann G., Jahreis K., Schmid K., Wrieden S., Lengeler J. W. 1991; Molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria. Molecular Microbiology 5:2913–2922
    [Google Scholar]
  2. Blatch G. L., Scholle R. R., Woods D. R. 1990; Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptakeencoding region. Gene 95:17–23
    [Google Scholar]
  3. Ferenci T., Kornberg H. L. 197l; Pathway of fructose utilization by Escherichia coli. FEBS Letters 13:127–130
    [Google Scholar]
  4. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  5. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  6. Loesche W. J. 1986; Role of Streptococcus mutans in human dental decay. Microbiological Reviews 50:353–380
    [Google Scholar]
  7. Macrina F. L., Evans R. P., Tobian J. A., Hartley D. L., Clewell D. B., Jones K. R. 1983; Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning. Gene 25:145–150
    [Google Scholar]
  8. Perry D., Kuramitsu H. K. 1989; Genetic linkage among cloned genes of Streptococcus mutans. Infection and Immunity 57:805–809
    [Google Scholar]
  9. Perry D., Wondrack L. M., Kuramitsu H. K. 1983; Genetic transformation of putative cariogenic properties in Streptococcus mutans. Infection and Immunity 41:722–727
    [Google Scholar]
  10. Porter E. V., Chassy B. M., Holmlund C. E. 1980; Partial purification and properties of a mannofructokinase from Streptococcus mutans SL-1. Infection and Immunity 30:43–50
    [Google Scholar]
  11. Rauch P. J. G., de Vos W. M. 1992; Transcriptional regulation of the Tn5276-located Lactococcus lactis sucrose operon and characterization of the sacA gene encoding sucrose-6-phosphate hydrolase. Gene 121:55–61
    [Google Scholar]
  12. St Martin E. J., Wittenberger C. L. 1979; Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans. Infection and Immunity 24:865–868
    [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  14. Sato Y., Kuramitsu H. K. 1988; Sequence analysis of the Streptococcus mutans scrB gene. Infection and Immunity 56:1956–1960
    [Google Scholar]
  15. Sato Y., Poy F., Jacobson G. R., Kuramitsu H. K. 1989; Characterization and sequence analysis of the scrA gene encoding enzyme IIScr of the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system. Journal of Bacteriology 171:263–271
    [Google Scholar]
  16. Sato Y., Yamamoto Y., Suzuki R., Kizaki H., Kuramitsu H. K. 1991; Construction of scrA :: lacZ gene fusions to investigate regulation of the sucrose PTS of Streptococcus mutans. FEMS Microbiology Letters 79:339–346
    [Google Scholar]
  17. Sato Y., Yamamoto Y., Kizaki H., Kuramitsu H. K. 1992; Cloning of the phospho-galactosidase gene in Escherichia coli from lactose-negative mutants of Streptococcus mutans isolated following random mutagenesis with plasmid pVA891 clone banks. FEMS Microbiology Letters 91:219–224
    [Google Scholar]
  18. Schmid K., Ebner R., Altenbuchner J., Schmitt R., Lengeler J. W. 1988; Plasmid-mediated sucrose metabolism in Escherichia coli K-12: mapping of the scr genes of pUR400. Molecular Microbiology 2:1–8
    [Google Scholar]
  19. Slee A. M., Tanzer J. M. 1982; Sucrose transport by Streptococcus mutans. Evidence for multiple transport systems. Biochimica et Biophysica Acta 692:415–424
    [Google Scholar]
  20. Sprenger G. A., Lengeler J. W. 1988; Analysis of sucrose catabolism in Klebsiella pneumoniae and in Scr+ derivatives of Escherichia coli K-12. Journal of General Microbiology 134:1635–1644
    [Google Scholar]
  21. Tabor S., Richardson C. C. 1985; A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proceedings of the National Academy of sciences of the United States of America 821074–1078
    [Google Scholar]
  22. Tanzer J. M., Chassy B. M., Krichevsky M. I. 1972; Sucrose metabolism by Streptococcus mutans SL-1. Biochimica et Biophysica Acta 261:379–387
    [Google Scholar]
  23. Thompson J., Sackrtt D. L., Donkersloot J. A. 1991; Purification and properties of fructokinase I from Lactococcus lactis Localization of scrK on the sucrose-nisin transposon Tn5306. Journal of Biological Chemistry 266:22626–22633
    [Google Scholar]
  24. Thompson J., Nguyen N. Y., Robrish S. A. 1992; Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport, catabolism, and products. Journal of Bacteriology 174:3227–3235
    [Google Scholar]
  25. Vieira J., Messing J. 1987; Production of single-stranded plasmid DNA. Methods in Enzymology 153:3–11
    [Google Scholar]
  26. Wehmeier U., Sprenger G. A., Lengeler J. W. 1989; The use of λplac-Mu hybrid phages in Klebsiella pneumoniae and the isolation of stable Hfr strains. Molecular and General Genetics 215:529–536
    [Google Scholar]
  27. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  28. Zembrzuski B., Chilco P., Liu X. L., Liu J., Conway T., Scopes R. 1992; Cloning, sequencing, and expression of the Zymomonas mobilis fructokinase gene and structural comparison of the enzyme with other hexose kinases. Journal of Bacteriology 174:3455–3460
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-5-921
Loading
/content/journal/micro/10.1099/00221287-139-5-921
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error