RT Journal Article SR Electronic(1) A1 Lodder, Stephen A1 Wood, David A1 Gull, KeithYR 1993 T1 A protoplasting technique with general applicability for molecular karyotyping of hymenomycetes JF Microbiology, VO 139 IS 5 SP 1063 OP 1067 DO https://doi.org/10.1099/00221287-139-5-1063 PB Microbiology Society, SN 1465-2080, AB Pulsed-field gel electrophoresis (PFGE) has been used to facilitate genetic fingerprinting within a natural hymenomycete population. A protoplasting technique has been developed which shows that the basidial cells of the commercially important mushrooms Agaricus bisporus, A. bitorquis and Lentinus edodes (Shiitake), as well as from many wild growing Agarics, are sensitive to commercially available fungal cell wall degrading enzymes. The technique produces a high yield of intact protoplasts within short time periods. Using the basidial cell protoplasting technique, chromosomal-sized DNA from a wild fruit body of Coprinus comatus has been prepared and resolved into distinct bands using PFGE. This protoplasting technique will allow the application of PFGE to provide electrophoretic karyotypes within natural and commercial populations of hymenomycetes., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-5-1063