Summary: Pulsed-field gel electrophoresis (PFGE) has been used to facilitate genetic fingerprinting within a natural hymenomycete population. A protoplasting technique has been developed which shows that the basidial cells of the commercially important mushrooms and (Shiitake), as well as from many wild growing Agarics, are sensitive to commercially available fungal cell wall degrading enzymes. The technique produces a high yield of intact protoplasts within short time periods. Using the basidial cell protoplasting technique, chromosomal-sized DNA from a wild fruit body of has been prepared and resolved into distinct bands using PFGE. This protoplasting technique will allow the application of PFGE to provide electrophoretic karyotypes within natural and commercial populations of hymenomycetes.


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