1887

Abstract

Pulsed-field gel electrophoresis (PFGE) has been used to facilitate genetic fingerprinting within a natural hymenomycete population. A protoplasting technique has been developed which shows that the basidial cells of the commercially important mushrooms and (Shiitake), as well as from many wild growing Agarics, are sensitive to commercially available fungal cell wall degrading enzymes. The technique produces a high yield of intact protoplasts within short time periods. Using the basidial cell protoplasting technique, chromosomal-sized DNA from a wild fruit body of has been prepared and resolved into distinct bands using PFGE. This protoplasting technique will allow the application of PFGE to provide electrophoretic karyotypes within natural and commercial populations of hymenomycetes.

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/content/journal/micro/10.1099/00221287-139-5-1063
1993-05-01
2021-07-24
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References

  1. Allen J.J., Moore D., Elliott T.J. 1992; Persistent meiotic arrest in basidia of Agaricus bisporus.. Mycological Research 96:125–127
    [Google Scholar]
  2. Barrett V., Lemke P.A., Dixon R.K. 1989; Protoplast formation from selected species of ectomycorrhizal fungi.. Applied Microbiology and Biotechnolog y 30331–387
    [Google Scholar]
  3. Barton R.C., Gull K. 1992; Isolation, characterization, and genetic analysis of monosomic, aneuploid mutants of Candida albicans.. Molecular Microbiology 6:171–177
    [Google Scholar]
  4. Binninger D.M., Skrzyn C., Pukkila P.J., Casselton L.A.C. 1987; DNA-mediated transformation of the basidiomycete Coprinus cinereus.. EMBO Journal 6:835–840
    [Google Scholar]
  5. Burrows B.M., Elliott T. J., Casselton L.A. 1990; DNA- mediated transformation of the secondarily homothallic basidiomycete Coprinus bilanatus.. Current Genetics 17:175–177
    [Google Scholar]
  6. De Vries O. M. H., Wessels J.G.H. 1972; Release of protoplasts from Schizophyllum commune by a lytic enzyme from Trichoderma viride.. Journal of General Microbiology 73:13–22
    [Google Scholar]
  7. Gull K., Moore P.M., Trinci A. P. J. 1972; Preparation of protoplasts from Geotrichum lactis and use of fluorescence to detect cell walls.. Transactions of the British Mycological Society 59:79–85
    [Google Scholar]
  8. Hamlyn P.F., Bradshaw R.E., Mellon F.M., Santiago C.M., Wilson J.M., Peberdy J.F. 1981; Efficient protoplast isolation from fungi using commercial enzymes.. Enzyme and Microbial Technology 3:321–325
    [Google Scholar]
  9. Iten W., Matile P. 1970; Role of chitinase and other lysosomal enzymes of Coprinus lagopus in the autolysis of fruiting bodies.. Journal of General Microbiology 61:301–309
    [Google Scholar]
  10. Kayser T., Wostemeyer J. 1991; Electrophoretic karyotype of the zygomycete Absidia glauca: evidence for differences between mating types.. Current Genetics 19:279–284
    [Google Scholar]
  11. Loftus M.G., Moore D., Elliott T.J. 1988; DNA polymorphisms in commercial and wild strains of the cultivated mushroom Agaricus bisporus.. Theoretical and Applied Genetics 76:712–718
    [Google Scholar]
  12. Moore D. 1975; Production of Coprinus protoplasts by use of chitinase or helicase.. Transactions of the British Mycology Society 65:134–136
    [Google Scholar]
  13. Peberdy J.F. 1979; Fungal protoplasts: isolation, reversion, and fusion.. Annual Review of Microbiology 33:21–39
    [Google Scholar]
  14. Peberdy J.F. 1989; Fungi without coats – protoplasts as tools for mycological research.. Mycological Research 93:1–20
    [Google Scholar]
  15. Royer J.C., Hintz W.E., Horgen P.A. 1991; Efficient protoplast formation and regeneration and electrophoretic karyotype analysis of Agaricus bisporus.. In Genetics and Breeding of Agaricus pp. 52–56 Van Griensven L.J.L.D. Edited by Wageningen: Pudoc;
    [Google Scholar]
  16. Royse D.J., May B. 1982; Use of isozyme variation to identify genotype classes of Agaricus brunnescens.. Mushroom Science 8:1–9
    [Google Scholar]
  17. Schulz-Weddigen I. 1982; Protoplasten aus Basidien und vegetativen Zellen von Coprinus radiatus.. Berichte Deutschen Botanischen Gesellschaft 95:431–440
    [Google Scholar]
  18. Sonnenberg A.S., Wessels J.G.H., Van Griensven L. J. 1988; An efficient protoplasting/regeneration system for Agaricus bisporus and Agaricus bitorquis.. Current Microbiology 17:285–291
    [Google Scholar]
  19. Spear M., Royse D.J. 1983; Formation, purification, and regeneration of protoplasts from Agaricus brunnescens.. Phytopathology 73:375
    [Google Scholar]
  20. Summerbell R.C., Castle A.J., Horgen P.A., Anderson J.B. 1989; Inheritance of restriction fragment length polymorphisms in Agaricus brunnescens.. Generics 123:293–300
    [Google Scholar]
  21. Walz M., Kuck U. 1991; Polymorphic karyotypes in related Acremonium strains.. Current Genetics 19:73–76
    [Google Scholar]
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