1887

Abstract

Summary: Compounds (e.g. -isohumulone) derived from flowers of the hop plant ( L.) protect beer from spoilage. Cells of IFO 3960 did not die when they were exposed to 40 μM--isohumulone for up to 120 h. At higher concentrations (80, 120 μM) death occurred after a lag period of about 30 h. -Isohumulone dissipated the transmembrane pH gradient of non-growing cells and reduced their ability to accumulate L-[U-C]leucine. The membrane potential was dissipated to a smaller extent. Addition of -isohumulone to cells that had accumulated L[U-C]leucine, under conditions in which no synthesis of protein took place, caused slow leakage of radiolabelled leucine. -Isohumulone did not inhibit the activity of the proton-translocating membrane ATPase. Potentiometric experiments with resting cell suspensions suggested that -isohumulone acted as an ionophore of the mobile-carrier type, causing electroneutral exchange of H for divalent cations such as Mn. A second monovalent cation (e.g. K) was essential for protonophoric activity.

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/content/journal/micro/10.1099/00221287-139-5-1041
1993-05-01
2021-05-16
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References

  1. Archibald F. S., Fridovich I. 1981; Manganese and defenses against oxygen toxicity in Lactobacillus plantarum. Journal of Bacteriology 145:442451
    [Google Scholar]
  2. Bender G. R., Sutton S. V. W., Marquis R. E. 1986; Acid tolerance, proton permeabilities, and membrane ATPases of oral streptococci. Infection and Immunity 53:331338
    [Google Scholar]
  3. De Man J. C., Rogosa M., Sharpe M. E. 1960; A medium for cultivation of lactobacilli. Journal of Applied Bacteriology 23:130135
    [Google Scholar]
  4. Harold F. M. 1986 The Vital Force: A Study of Bioenergetics New York: W. H. Freeman;
    [Google Scholar]
  5. Harold F. M., Baarda J. R. 1968; Inhibition of membrane transport in Streptococcus faecalis by uncouplers of oxidative phosphorylation and its relationship to proton conduction. Journal of Bacteriology 96:20252034
    [Google Scholar]
  6. Hudson J. R., Rudin A. D. 1959; Metal derivatives of isohumulone. Journal of the Institute of Brewing 65:414–418
    [Google Scholar]
  7. Kashket E. R. 1987; Bioenergetics of lactic acid bacteria: cyto-plasmic pH and osmotolerance. FEMS Microbiology Reviews 46:233244
    [Google Scholar]
  8. Lundin A. 1984; Extraction and automatic luminometric assay of ATP, ADP and AMP. In Analytical Applications of Bioluminescence and Chemiluminescence pp. 491–501 Kricka L. E., Stanley P. E., Thorpe G. H. G., Whitehead T. P. Edited by London: Academic Press;
    [Google Scholar]
  9. Pfeiffer D. R., Reed P. W., Lardy H. A. 1974; Ultraviolet and fluorescent spectral properties of the divalent cation ionophore A23187 and its metal ion complexes. Biochemistry 13:4007–40l4
    [Google Scholar]
  10. Poolman B., Driessen A. J. M., Konings W. N. 1987; Regulation of solute transport in streptococci by external and internal pH values. Microbiological Reviews 51:498508
    [Google Scholar]
  11. Schmalreck A. F., Teuber M., Reininger W., Hartl A. 1975; Structural features determining the antibiotic potencies of natural and synthetic hop bitter resins, their precursors and derivatives. Canadian Journal of Microbiology 21:205–212
    [Google Scholar]
  12. Serrano R. 1978; Characterisation of the plasma membrane ATPase of Saccharomyces cereuisiae. Molecular and Cellular Biochemistry 22:51–63
    [Google Scholar]
  13. Simpson W. J. 1991 Molecular Structure and Antibacterial Function of Hop Resin Materials PhD Thesis Council for National Academic Awards; UK:
    [Google Scholar]
  14. Simpson W. J., Hammond J. R. M. 1989; Cold ATP extractants compatible with constant light signal firefly luciferase reagents. In ATP Luminescence: Rapid Methods in Microbiology. Society for Applied Bacteriology Technical Series 26 pp. 4552 Stanley P. E., Mccarthy B. J., Smither R. Edited by Oxford: Blackwell Scientific Publications.;
    [Google Scholar]
  15. Simpson W. J., Hammond J. R. M. 1991; Antibacterial action of hop resin materials. In European Brewery Convention, Proceedings of the 23rd Congress, Lisbon pp. 185192 London: IRL Press;
    [Google Scholar]
  16. Simpson W. J., Smith A. R. W. 1992; Factors affecting antibacterial activity of hop compounds and their derivatives. Journal of Applied Bacteriology 72:327334
    [Google Scholar]
  17. Spector T. 1978; Refinement of the Coomassie blue method of protein quantification. Analytical Biochemistry 86:142146
    [Google Scholar]
  18. Ten Brink B., Otto R., Hansen U. -P., Konings W. N. 1985; Energy recycling by lactate efflux in growing and nongrowing cells of Streptococcus lactis. Journal of Bacteriology 162:383390
    [Google Scholar]
  19. Teuber M., Schmalreck A. F. 1973; Membrane leakage in Bacillus subtilis 168 induced by the hop constituents lupulone, humulone, isohumulone and humulinic acid. Archives of Microbiology 94:159171
    [Google Scholar]
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