Summary: 5-Aminosalicylate (5AS) was converted to L-malate, pyruvate and ammonia by cell-free extracts from sp. BN9 in the presence of glutathione. In the absence of glutathione, 5AS was oxidized to the ringfission product -4-amino-6-carboxy-2-oxo-hexa-3,5-dienoate (-ACOHDA). Glutathione catalysed the spontaneous isomerization of -ACOHDA to its -isomer. The same reaction was catalysed by light and by acidic conditions. -ACOHDA was enzymically deaminated to fumarylpyruvate (-2,4-dioxo-5-hexenoate). The -ACOHDA hydrolase was induced after growth of sp. BN9 with 5AS, but not after growth with acetate or nutrient broth. At the fumarylpyruvate stage, the metabolism of 5AS converged with a pathway described for the degradation of gentisate. Fumarylpyruvate was cleaved by sp. BN9 to fumarate and pyruvate.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error