Streptomyces coelicolor (Müller) became resistant to killing by hydrogen peroxide (H2O2) when pretreated with non-lethal concentrations of H2O2. When rapidly growing cells were pretreated with 100 μm-H2O2, they became 7–10-fold more resistant to 20 mm-H2O2 than were naive cells. Activities of several oxidative defense enzymes were measured in cells treated with 100 μm-H2O2 in either exponential or stationary phase growth. The specific activity of catalase in crude extracts of cells pretreated in either phase increased about 40%, Peroxidase activity, in cell extracts and culture supernatants, respectively, of cells treated in the stationary growth phase increased two times and four times. Glucose-6-phosphate dehydrogenase increased by 60% at the exponential growth phase. Glutathione reductase increased 80% after treatment in the exponential phase and 4-fold in the stationary growth phase. However, superoxide dismutase activity decreased by 70%. Two mutants resistant to H2O2 were isolated after mutagenesis of spores with N-methyl-N′-nitro-N-nitrosoguanidine. In addition to a dramatic increase in the survival rate in 20 mm-H2O2, both mutants exhibited increased activities of all the above enzymes except superoxide dismutase. The pleiotropic phenotype of the mutants suggests that there exists a global regulation of oxidative response in S. coelicolor.
BeersR. F.JrSizerI. W.1952; A spectrophotometric method for measuring breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry 195:276–287
ChristmanM. F.,
MorganR. W.,
JacobsonF. S.,
AmesB. M.1985; Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium. Cell 41:753–762
ChristmanM. F.,
StorzG.,
AmesB. M.1989; OxyR, a positive regulator of hydrogen peroxide-inducible genes in Escherichia coli and Salmonella typhimurium, is homologous to a family of bacterial regulatory proteins. Proceedings of the National Academy of Sciences of the United States of America 86:3484–3488
DowdsB. C. A.,
MurphyP.,
McconnellD. J.,
DevineK. M.1987; Relationship among oxidative stress, growth cycle, and sporulation in Bacillus subtilis. Journal of Bacteriology 169:5771–5775
FarrB. S.,
TouatiD.,
KogomaT.1988; Effects of oxygen stress on membrane functions in Escherichia coli: role of HPI catalase. Journal of Bacteriology 114:543–548
GreenbergJ. T.,
DempleB.1989; A global response induced in Escherichia coli by redox-cycling agents overlaps with that induced by peroxide stress. Journal of Bacteriology 171:3933–3939
HalliwellB.,
GutteridgeJ. M.1989; Protection against oxidants in biological systems: the superoxide theory of oxygen toxicity. In Free Radicals in Biology and Medicine pp. 86–187HalliwellB.,
GutteridgeJ. M.
Edited by Oxford: Oxford University Press;
HassanH. M.,
FridovichI.1977; Enzymatic defense against the toxicity of oxygen and of streptomycin in Escherichia coli. Journal of Bacteriology 129:1574–1583
HassettD. J.,
CohenM. S.1989; Bacterial adaptation to oxidative stress: implication for pathogenesis and interaction with phagocytic cells. FASEB Journal 3:2574–2582
JenkinsD. E.,
SchultzJ. E.,
MatinS.1988; Starvation-induced cross protection against heat and hydrogen challenge in Escherichia coli. Journal of Bacteriology 170:3910–3914
JonesK. L.1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. Jouma of Bacteriology 57:141–145
KapoorM.,
SveenivasanG. M.1988; The heat shock response of Neurospora crassa: stress-induced thermotolerance in relation to peroxidase and superoxide dismutase levels. Biochemical and Biophysical Research Communications 156:1097–1102
MorganR. W.,
ChristmanM. F.,
JacobsonF. S.,
StorzG.,
AmesB. N.1986; Hydrogen peroxide-inducible proteins in Salmonella typhimurium overlap with heat shock and other stress proteins. Proceedings of the National Academy of Sciences of the United States of America 83:8059–8063
MurphyP.,
DowdsB. C. A.,
McconnellD. J.,
DevineK. M.1987; Oxidative stress and growth temperature in Bacillus subtilis. Journal of Bacteriology 169:5766–5770
SmithI. K.,
VierhellerT. L.,
ThroneC. A.1988; Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Analytical Biochemistry 175:408–413
StorzG.,
JacobsonF. S.,
TartagliaL. A.,
MorganR. W.,
SilveriaL. A.,
AmesB. N.1989; An alkyl hydroperoxide reductase induced by oxidative stress in Salmonella typhimurium and Escherichia coli: genetic characterization and cloning of ahp. Journal of Bacteriology 171:2049–2055
TouatiD.1988; Transcriptional and posttranscriptional regulation of manganese superoxide dismutase biosynthesis in Escherichia coli, studied with operon and protein fusions. Journal of Bacteriology 170:2511–2520
VanBogelenR. A.,
KellyP. M.,
NeidhardtF. C.1987; Differential induction of heat shock, SOS and oxidation stress regulons and accumulation of nucleotides in E. coli. Journal of Bacteriology 169:26–32
WuJ.,
WeissB.1991; Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of E. coli. Journal of Bacteriology 173:2864–2871