%0 Journal Article %A Lee, Jong-Soo %A Hah, Yung-Chil %A Roe, Jung-Hye %T The induction of oxidative enzymes in Streptomyces coelicolor upon hydrogen peroxide treatment %D 1993 %J Microbiology, %V 139 %N 5 %P 1013-1018 %@ 1465-2080 %R https://doi.org/10.1099/00221287-139-5-1013 %I Microbiology Society, %X Streptomyces coelicolor (Müller) became resistant to killing by hydrogen peroxide (H2O2) when pretreated with non-lethal concentrations of H2O2. When rapidly growing cells were pretreated with 100 μm-H2O2, they became 7–10-fold more resistant to 20 mm-H2O2 than were naive cells. Activities of several oxidative defense enzymes were measured in cells treated with 100 μm-H2O2 in either exponential or stationary phase growth. The specific activity of catalase in crude extracts of cells pretreated in either phase increased about 40%, Peroxidase activity, in cell extracts and culture supernatants, respectively, of cells treated in the stationary growth phase increased two times and four times. Glucose-6-phosphate dehydrogenase increased by 60% at the exponential growth phase. Glutathione reductase increased 80% after treatment in the exponential phase and 4-fold in the stationary growth phase. However, superoxide dismutase activity decreased by 70%. Two mutants resistant to H2O2 were isolated after mutagenesis of spores with N-methyl-N′-nitro-N-nitrosoguanidine. In addition to a dramatic increase in the survival rate in 20 mm-H2O2, both mutants exhibited increased activities of all the above enzymes except superoxide dismutase. The pleiotropic phenotype of the mutants suggests that there exists a global regulation of oxidative response in S. coelicolor. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-5-1013