Summary: Fatty acid methyl ester (FAME) derivatives were examined as a means of characterizing isolates and distinguishing them from other spirochaetes. Analysis was performed using a gas liquid chromatography column in conjunction with Microbial Identification System (MIS) software. Reproducible FAME profiles were produced which distinguished species, and . Furthermore, the FAME profiles of four recognized species (including two American isolates of , B31 and JD1) were distinct from one another and from the BSK II medium in which they were grown. The results confirm previous reports that FAME profiles of bacteria represent a diagnostic phenotypic property and suggest that they may have applications in the chemotaxonomic classification of species.


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