1887

Abstract

The need for consistent nomenclature and accurate assessment of late growth phases in diauxic yeast cultures is highlighted by the substantial variation of stress tolerance in after the exhaustion of the initial fermentable carbon source. At present, a wide variety of assessment methods and confused terminology exists in the literature, leading to difficulties in the interpretation and comparison of published results. A method based on the depletion of ethanol accumulated during the respiro-fermentative growth phase is suggested as suitable for assessing subsequent growth phases and reporting results. Consistent application of nomenclature for growth phases is recommended to assist the interpretation of published experimental results. It is suggested that the phases of growth in diauxic batch culture should be referred to using the terms (1) initial lag phase, (2) respirofermentative phase, (3) diauxic lag phase, (4) respiratory phase, (5) stationary phase, and (6) death phase.

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/content/journal/micro/10.1099/00221287-139-4-835
1993-04-01
2024-04-25
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References

  1. Attfield P. V. 1987; Trehalose accumulates in Saccharomyces cerevisiae during exposure to agents that induce heat shock response. FEBS Letters 225:259–263
    [Google Scholar]
  2. Bataillé N., Régnacq M., Boucherie H. 1991; Induction of a heat-shock-type response in Saccharomyces cerevisiae following glucose limitation. Yeast 7:367–378
    [Google Scholar]
  3. Blomberg A., Larsson C., Gustafsson L. 1988; Microcalorimetric monitoring of growth of S. cerevisiae: osmo-tolerance in relation to physiological state. Journal of Bacteriology 170:4562–4568
    [Google Scholar]
  4. Boucherie H. 1985; Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyces cerevisiae. Journal of Bacteriology 161:385–392
    [Google Scholar]
  5. Eraso P., Cid A., Serrano R. 1987; Tight control of the amount of yeast plasma membrane ATPase during changes in growth conditions and gene dosage. FEBS Letters 224:193–197
    [Google Scholar]
  6. Francois J., Neves M.-J., Hers H.-G. 1991; The control of trehalose biosynthesis in Saccharomyces cerevisiae: evidence for a catabolite inactivation and repression of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase. Yeast 7:575–587
    [Google Scholar]
  7. Hottiger T., Schmutz P., Wiemken A. 1987; Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae. Journal of Bacteriology 169:5518–5522
    [Google Scholar]
  8. Larsson C., Gustafsson L. 1987; Glycerol production in relation to the ATP pool and heat production rate of the yeasts Debaryomyces hansenii and Saccharomyces cerevisiae during salt stress. Archives of Microbiology 147:358–363
    [Google Scholar]
  9. Mackenzie K. F., Singh K. K., Brown A. D. 1988; Water stress plating hypersensitivity of yeasts: protective role of trehalose in Saccharomyces cerevisiae. Journal of General Microbiology 134:1661–1666
    [Google Scholar]
  10. Meyer E. D., Sinclair N. A., Nagy B. 1975; Comparison of the survival and metabolic activity of psychrophilic and mesophilic yeasts subjected to freeze-thaw stress. Applied Microbiology 29:739–744
    [Google Scholar]
  11. Parry J. M., Davies P. J., Evans W. E. 1976; The effects of ‘cell age’ upon the lethal effects of chemical and physical mutagens in the yeast, Saccharomyces cerevisiae. Molecular and General Genetics 146:27–35
    [Google Scholar]
  12. Panaretou B., Piper P. W. 1990; Plasma-membrane ATPase action affects several stress tolerances of Saccharomyces cerevisiae and Schizosaccharomyces pombe as well as the extent and duration of the heat shock response. Journal of General Microbiology 136:1763–1770
    [Google Scholar]
  13. Scheper T., Hoffmann H., Schügerl K. 1987; Flow cytometric studies during culture of Saccharomyces cerevisiae. Enzyme and Microbial Technology 9:399–405
    [Google Scholar]
  14. Van Laere A. 1989; Trehalose, reserve and/or stress metabolite?. FEMS Microbiology Reviews 63:201–210
    [Google Scholar]
  15. De Virgilio C., Burckert N., Boller T., Wiemken A. 1991; A method to study the rapid phosphorylation-related modulation of neutral trehalase activity by temperature shifts in yeast. FEBS Letters 291:355–358
    [Google Scholar]
  16. Warth A. D. 1989; Transport of benzoic and propanoic acids by Zygosaccharomyces bailii. Journal of General Microbiology 135:1383–1390
    [Google Scholar]
  17. Watson K. 1987; Temperature relations. In The Yeasts, 2nd edn. 2 pp. 41–71 Edited by Rose A. H., Harrison J. C. London: Academic Press;
    [Google Scholar]
  18. Winkler K., Kienle I., Burgert M., Wagner J.-C., Holzer H. 1991; Metabolic regulation of the trehalose content of vegetative yeast. FEBS Letters 291:269–272
    [Google Scholar]
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