1887

Abstract

The gene encoding cyclohexadienyl dehydratase from , designated , was cloned in and sequenced recently by Zhao . ( 267, 2487–2493, 1992). N-Terminal sequencing of the purified cyclohexadienyl dehydratase yielded a run of 11 residues which matched perfectly with the deduced amino acid residues 26–36. This showed that a 25 residue peptide was cleaved from the N-terminus of a preprotein formed in . The amino acid composition of the 25 residue peptide was typical of signal sequences for periplasmic proteins. Most or all of the cyclohexadienyl dehydratase was released from and carrying the gene following spheroplast formation, osmotic shock or chloroform treatment. The location of the enzyme in the periplasm of both and was confirmed by Western blotting analysis using antibody prepared against PheC. Electron microscopy using immunogold labelling showed an apparent localization of cyclohexadienyl dehydratase at the polar regions of the periplasmic space in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-4-807
1993-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/4/mic-139-4-807.html?itemId=/content/journal/micro/10.1099/00221287-139-4-807&mimeType=html&fmt=ahah

References

  1. Ahmad S., Jensen R. A. 1988; New prospects for deducing the evolutionary history of metabolic pathways in prokaryotes: aromatic biosynthesis as a case-in-point. Origins of Life and Evolution of the Biosphere 18:41–57
    [Google Scholar]
  2. Ahmad S., Weisburg W. G., Jensen R. A. 1990; Evolution of aromatic amino acid biosynthesis and application to the fine-tuned phylogenetic positioning of enteric bacteria. Journal of Bacteriology 172:1051–1061
    [Google Scholar]
  3. Ames G.F.-L., Prody C., Kustu S. 1984; Simple, rapid, and quantitative release of periplasmic proteins by chloroform. Journal of Bacteriology 160:1181–1183
    [Google Scholar]
  4. Baldwin G. S., Davidson B. E. 1981; A kinetic and structural comparison of chorismate mutase/prephenate dehydratase from mutant strains of E. coli K-12 defective in the PheA gene. Archives of Biochemistry and Biophysics 211:66–75
    [Google Scholar]
  5. Berry A., Bhatnagar R. K., Jensen R. A. 1987; Enzymic basis for leakiness of auxotrophs for phenylalanine in Pseudomonas aeruginosa. Journal of General Microbiology 133:3257–3263
    [Google Scholar]
  6. Birdsell D. C., Cota-Robles E. H. 1967; Production and ultrastructure of lysozyme and ethylene-diaminetetraacetate-lysozyme spheroplasts of Escherichia coli. Journal of Bacteriology 93:427–437
    [Google Scholar]
  7. Bonner C. A., Fischer R. S., Ahmad S., Jensen R. A. 1990; Remnants of an ancient pathway to L-phenylalanine and L-tyrosine in enteric bacteria: evolutionary implications and biotechnological impact. Applied and Environmental Microbiology 56:3741–3747
    [Google Scholar]
  8. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  9. Brickman E., Beckwith J. 1975; Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and transducing phages. Journal of Molecular Biology 96:307–316
    [Google Scholar]
  10. Byng G. S., Kane J. F., Jensen R. A. 1982; Diversity in the routing and regulation of complex biochemical pathways as indicators of microbial relatedness. Critical Reviews in Microbiology 9:227–252
    [Google Scholar]
  11. Cheng K.-J., Ingram J. M., Costerton J. W. 1970; Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and of pH. Journal of Bacteriology 104:748–753
    [Google Scholar]
  12. Cotton R. G. H., Gibson F. 1965; The biosynthesis of phenylalanine and tyrosine: enzymes converting chorismic acid into prephenic acid and their relationships to prephenate dehydatase and prephenate dehydrogenase. Biochimica et Biophysica Acta 100:76–88
    [Google Scholar]
  13. Davis B. D. 1953; Autocatalytic growth of a mutant due to accumulation of an unstable phenylalanine precursor. Science 118:251–252
    [Google Scholar]
  14. Fischer R., Jensen R. A. 1987; Arogenate dehydratase. Methods in Enzymology 142:495–502
    [Google Scholar]
  15. Fischer R. S., Zhao G. S., Jensen R. A. 1991; Cloning, sequencing, and expression of the P-protein gene (pheA) of Pseudomonas stutzeri in Escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis. Journal of General Microbiology 137:1293–1301
    [Google Scholar]
  16. Fiske M. J., Whitaker R. J., Jensen R. A. 1983; Hidden overflow pathway to L-phenylalanine in Pseudomonas aeruginosa. Journal of Bacteriology 154:623–631
    [Google Scholar]
  17. Hiebert E., Purcifull D. E., Christie R. G. 1984; Purification and immunological analysis of plant viral inclusion bodies. Methods in Virology 8:225–280
    [Google Scholar]
  18. Holloway B. W. 1955; Genetic recombination in Pseudomonas aeruginosa. Journal of General Microbiology 13:572–581
    [Google Scholar]
  19. Jensen R. A., Fischer R. S. 1987; The postprephenate biochemical pathways to phenylalanine and tyrosine: an overview. Methods in Enzymology 142:472–478
    [Google Scholar]
  20. Jung E., Zamir L. O., Jensen R. A. 1986; Chloroplasts of higher plants synthesize l-phenylalanine via l-arogenate. Proceedings of the National Academy of Sciences of the United States of America 83:7231–7235
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  23. Neu H. C., Heppel L. A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 40:3685–3692
    [Google Scholar]
  24. Nishioka M., Demerec M., Eisenstarck A. 1967; Genetic analysis of aromatic mutants of Salmonella typhimurium. Genetics 56:341–351
    [Google Scholar]
  25. Oliver D. B. 1986 In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp. 56–69 Neidhardt F. C., Bhattacharya SK. Edited by and others Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Patel N., Pierson D. L., Jensen R. A. 1977; Dual enzymatic routes to l-tyrosine and l-phenylalanine via pretyrosine in Pseudomonas aeruginosa. Journal of Biological Chemistry 252:5839–5846
    [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  28. Wilkinson S. G. 1967; The sensitivity of pseudomonads to ethylenediaminetetraacetic acid. Journal of General Microbiology 47:67–76
    [Google Scholar]
  29. Woodruff W. A., Hancock R. E. W. 1988; Construction and characterization of Pseudomonas aeruginosa protein F-deficient mutants after in vitro and in vivo insertion mutagenesis of the cloned gene. Journal of Bacteriology 170:2592–2598
    [Google Scholar]
  30. Xia T., Jensen R. A. 1992; Monofunctional chorismate mutase from Serratia rubidaea: a paradigm system for the three-isozyme gene family of enteric bacteria. Archives of Biochemistry and Biophysics 294:147–153
    [Google Scholar]
  31. Xia T., Ahmad S., Zhao G., Jensen R. A. 1991; A single cyclohexadienyl dehydratase specifies the prephenate dehydratase and arogenate dehydratase components of one of two independent pathways to l-phenylalanine in Erwinia herbicola. Archives of Biochemistry and Biophysics 286:461–465
    [Google Scholar]
  32. Zhao G., Xia T., Fischer R. S., Jensen R. A. 1992; Cyclohexadienyl dehydratase from Pseudomonas aeruginosa: molecular cloning of the gene and characterization of the gene product. Journal of Biological Chemistry 267:2487–2493
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-4-807
Loading
/content/journal/micro/10.1099/00221287-139-4-807
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error