1887

Abstract

Summary: The mutation of the double-stranded-RNA-specific ribonuclease III (RNAaseIII) was previously isolated by virtue of the lethal expression of RNAaseIII in . Here we show that is a single point mutation causing the substitution of glycine 97 by glutamic acid. The mutation eliminates the lethal phenotype of RNAaseIII expression in yeast and reduces fourfold the effect of RNAaseIII expression on bacteriophage gy1 propagation in . Mutant RNAaseIII-G97E and wild-type RNAaseIII were purified according to published procedures. The apparent molecular masses of the two enzymes on SDS polyacrylamide gels are the same but they differ in pI (6.85 for RNAaseIII-G97E and 7.3 for RNAaseIII). Whereas the two enzymes (under standard assay conditions) do not show a great difference in activity towards double-stranded RNA and defined single-stranded RNAaseIII substrates, they differ dramatically (20-fold or more) under conditions of Mg limitation. The hypothesis that limitation of Mg ions is responsible for the phenotypes of the mutation in and is discussed.

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/content/journal/micro/10.1099/00221287-139-4-717
1993-04-01
2019-10-17
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-4-717
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