1887

Abstract

The operon of codes for the synthesis of ribosomal proteins L28 and L33. In one mutant strain (TP28), these two proteins are made at about half their normal rates, ribosome assembly is greatly perturbed and precursor particles accumulate. The mutation in strain TP28 is in a Shine-Dalgarno sequence in the leader region of the messenger RNA. Another mutant, strain AM108, makes neither protein because it has the mobile element IS inserted into the coding sequence. Surprisingly, ribosome assembly in this strain is virtually normal with respect to growth rate. Strain AM90, which fails to make protein L33, has the element IS inserted into and also shows no major defects in ribosome assembly.

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1993-04-01
2021-10-27
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