Summary: The anthranilate synthase () genes in ISP5230 were located by allowing a segregationally unstable plasmid carrying cloned DNA and a thiostrepton resistance () marker to integrate into the chromosome. The integrated was mapped by conjugation and transduction to a location close to -2, between -6 and . A genomic DNA fragment containing from ISP5230 was cloned by complementation of a mutation in . Evidence from restriction enzyme analysis of the cloned DNA fragments, from Southern hybridization using the cloned DNA as probes, and from cotransduction frequencies, placed at a distance of 12–45 kb from the cluster. The overall arrangement of tryptophan biosynthesis genes in the chromosome differs from that in other bacteria examined so far.


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