Eikenella corrodens is a Gram-negative microaerophilic rod which is gaining recognition as an important human pathogen. We have previously reported the cloning and expression in Escherichia coli of a 3·6 kb Eik. corrodens genomic DNA fragment which encodes a 31·5 kDa haemagglutinin. Maxicell analysis revealed that this fragment also encodes two proteins of approximately 14 kDa. Nucleotide sequencing of the 2·2 kb fragment upstream of the haemagglutinin gene revealed two open reading frames with strong homology to genes encoding pilin subunit proteins of the type 4 or N-methylphenylalanine class. The two pilin genes, ecpA and ecpB, are complete and are expressed in E. coli. Southern analysis of ten additional Eik. corrodens strains revealed that all possess fragments homologous to ecpA. These data represent the first molecular evidence for pili in E. corrodens.
AndersonB. J.,
BillsM. M.,
EgertonJ. R.,
MattickJ. S.1984; Cloning and expression in Eschericha coli of the gene encoding the structural subunit of Bacteroides nodosus fimbriae. Journal of Bacteriology 160:748–754
BrooksG. G.,
O’DonoghueJ. M.,
RissingJ. P.,
SoapesK.,
SmithJ. W.1974; Eikenella corrodens, a recently recognized pathogen: infections in medical-surgical patients and in association with methylphenidate abuse. Medicine 53:325–342
DeckerM. D.,
GrahamB. S.,
HunterE. B.,
LiebowitzS. M.1986; Endocarditis and infections of intravascular devices due to Eikenella corrodens. American Journal of the Medical Sciences 292:209–212
DzinkJ. L.,
TannerA. C. R.,
HaffajeeA. D.,
SocranskyS. S.1985; Gram-negative species associated with active destructive periodontal lesions. Journal of Clinical Periodontology 12:648–659
EikenM.1958; Studies on an anaerobic rod-shaped Gram-negative microorganism: Bacteroides corrodens, n. sp. Acta Pathologica et Microbiologica Scandinavica 43:404–416
EllemanT. C.1988; Pilins of Bacteroides nodosus: molecular basis of serotypic variation and relationships to other bacterial pilins. Microbiological Reviews 52:233–247
EllemanT. C.,
HoyneP. A.1984; Nucleotide sequence of the gene encoding pilin of Bacteroides nodosus, the causal organism of ovine footrot. Journal of Bacteriology 160:1184–1187
FarringtonM.,
EykynS. J.,
WalkerM.,
WarrenR. E.1983; Vertebral osteomyelitis due to coccobacilli of the HB group. British Medical Journal 287:1658–1660
FinneyK. G.,
EllemanT. C.,
StewartD. J.1988; Nucleotide sequence of the pilin gene of Bacteroides nodosus 340 (serogroup D) and implications for the relatedness of serogroups. Journal of General Microbiology 134:575–584
GrosjeanH.,
FiersW.1982; Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene 18:199–209
JacksonF. L.,
GoodmanY. E.1984; Genus Eikenella. In Bergey’s Manual of Systematic Bacteriology1 pp. 597–600KriegN. R.,
HoltJ. G.,
HoltJ. G.
Edited by Baltimore: Williams Wilkins;
JacksonF. L.,
GoodmanY. E.,
BelF. R.,
WongP. C.,
WhitehouseR. L. S.1971; Taxonomic status of facultative and strictly anaerobic ‘corroding bacilli’ that have been classified as Bacteroides corrodens. Journal of Medical Microbiology 4:171–184
JohnsonK.,
ParkerM. L.,
LoryS.1986; Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes. Journal of Biological Chemistry 261:15703–15708
LobetY.,
PeacockM. G.,
CieplakW. J.1989; Frame-shift mutation in the lacZ of certain commercially available pUC18 plasmids. Nucleic Acids Research 17:4897
MarrsC. F.,
SchoolnikG.,
KoomeyJ. M.,
HardyJ.,
RothbardJ.,
FalkowS.1985; Cloning and sequencing of a Moraxella bovis pilin gene. Journal of Bacteriology 163:132–139
MarrsC. F.,
RuehlW.,
SchoolnikG. K.,
FalkowS.1988; Pilin gene phase variation of Moraxella bovis is caused by an inversion of the pilin genes. Journal of Bacteriology 170:3032–3039
MeyerT. F.,
BillyardE.,
HaasR.,
StorzbachS.,
SoM.1984; Pilus genes of Neisseria gonorrhoeae: chromosomal organization and DNA sequence. Proceedings of the National Academy of Sciences of the United States of America 81:6110–6114
PasloskeB. L.,
SastryP. A.,
FinlayB. B.,
ParanchychW.1988; Two unusual pilin sequences from different isolates of Pseudomonas aeruginosa. Journal of Bacteriology 170:3738–3741
PasloskeB. L.,
ScrabaD. G.,
ParanchychW.1989; Assembly of mutant pilins in Pseudomonas aeruginosa: formation of pili composed of heterologous subunits. Journal of Bacteriology 171:2142–2147
RaoV. K.,
WhitlockJ. A.,
Progulske-FoxA.1993; Cloning, characterization and sequencing of two haemagglutinin genes from Eikenella corrodens. Journal of General Microbiology 139:639–650
SastryP. A.,
FinlayB. B.,
PasloskeB. L.,
ParanchychW.,
PearlstoneJ. R.,
SmillieL. B.1985; Comparative studies on the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO. Journal of Bacteriology 164:571–577
ShawC. E.,
TaylorR. K.1990; Vibrio cholerae 0395 tcpA pilin gene sequence and comparison of predicted protein structural features to those of type 4 pilins. Infection and Immunity 58:3042–3049
StoloffA. L.,
GilliesM. L.1986; Infections with Eikenella corrodens in a general hospital: a report of 33 cases. Reviews of Infectious Diseases 8:50–53
TannerA. C. R.,
HafferC.,
BratthalG. T.,
ViscontiR. A.,
SocranskyS. S.1979; A study of the bacteria associated with advancing periodontitis in man. Journal of Clinical Periodontology 6:278–307
TønjumT.,
MarrsC. F.,
RozsaF.,
BøvreK.1991; The type 4 pilin of Moraxella nonliquefaciens exhibits unexpected similarities with the pilins of Neisseria gonorrhoeae and Bacteroides nodosus. Journal of General Microbiology 137:2483–2490
WoodsD. E.,
BassJ. A.,
JohansonW. G. J.,
StrausD. C.1980; Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients. Infection and Immunity 30:694–699