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Volume 139, Issue 3

Cloning and Sequencing of Two Type 4 (-Methylphenylalanine) Pilin Genes from Free

Abstract

is a Gram-negative microaerophilic rod which is gaining recognition as an important human pathogen. We have previously reported the cloning and expression in of a 3·6 kb genomic DNA fragment which encodes a 31·5 kDa haemagglutinin. Maxicell analysis revealed that this fragment also encodes two proteins of approximately 14 kDa. Nucleotide sequencing of the 2·2 kb fragment upstream of the haemagglutinin gene revealed two open reading frames with strong homology to genes encoding pilin subunit proteins of the type 4 or -methylphenylalanine class. The two pilin genes, and , are complete and are expressed in Southern analysis of ten additional strains revealed that all possess fragments homologous to . These data represent the first molecular evidence for pili in .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1993
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1993-03-01
2022-08-16
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Cloning and Sequencing of Two Type 4 (N-Methylphenylalanine) Pilin Genes from Eikenella Corrodens
Microbiology 139, 651 (1993); https://doi.org/10.1099/00221287-139-3-651
/content/journal/micro/10.1099/00221287-139-3-651
/content/journal/micro/10.1099/00221287-139-3-651
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