1887

Abstract

A specific polyclonal antiserum was prepared against a gel-purified 60 kDa extracellular protein of ATCC 19111 corresponding to protein p60 previously detected in culture broths of strains Mackaness and EGD [Kuhn, M. & Goebel, W. (1989), 57, 55–61]. Indirect immunogold labelling combined with transmission electron microscopy and high-resolution scanning electron microscopy were used toinvestigate the location and distribution of p60 on the bacterial cell surface. In bacteria grown to the early stationary phase about 25% of the extracellular protein was estimated to be associated with the cell surface. The anti-p60 antiserum proved to be -specific. In an indirect immunofluorescence test the antiserum reacted with strains representing all species and different serotypes, except and . No immunological cross-reactions were observed with 27 strains of bacteria from 16 other genera. The value of the anti-p60 antiserum in developing a diagnostic assay for cells in environmental samples and foods is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-3-609
1993-03-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/3/mic-139-3-609.html?itemId=/content/journal/micro/10.1099/00221287-139-3-609&mimeType=html&fmt=ahah

References

  1. Brown M. R. W., Williams P. 1985; The influence of environment on envelope properties affecting survival of bacteria in infections. Annual Review of Microbiology 39:527–556
    [Google Scholar]
  2. Buhnia A. K., Johnson M. G. 1992; Monoclonal antibody specific for Listeria monocytogenes associated with a 66-kilodalton cell surface antigen. Applied and Environmental Microbiology 58:1924–1929
    [Google Scholar]
  3. Butman B. T., Plank M. C., Durham R. J., Mattingly J. A. 1988; Monoclonal antibodies which identify a genus-specific Listeria antigen. Applied and Environmental Microbiology 54:1564–1569
    [Google Scholar]
  4. Cossart P., Mengaud J. 1989; Listeria monocytogenes - a model system for the molecular study of intracellular parasitism. Molecular Biology and Medicine 6:463–474
    [Google Scholar]
  5. Cottin J., Loiseau O., Robert R., Mahaza C., Carbonnelle B., Senet J. M. 1990; Surface Listeria monocytogenes carbohydrate binding components revealed by agglutination with neoglycoproteins. FEMS Microbiology Letters 68:301–306
    [Google Scholar]
  6. Cowart R. E., Lashmet J., Mcintosh M. E., Adams T. J. 1990; Adherence of a virulent strain of Listeria monocytogenes to the surface of a hepatocarcinoma cell line via lectin-substrate interaction. Archives of Microbiology 153:282–286
    [Google Scholar]
  7. Dabiri G. A., Sanger J. M., Portnoy D. A., Southwick F. S. 1990; Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly. Proceedings of the National Academy of Sciences of the United States of America 87:6068–6072
    [Google Scholar]
  8. Domann E., Wehland J., Rohde M., Pistor S., Hartl M., Goebel W., Leimeister-Wächter M., Wuenscher M., Chakraborty T. 1992; A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin. EMBO Journal 11:1981–1990
    [Google Scholar]
  9. Fiedler F., Ruhland G. J. 1987; Structure ofListeria monocytogenes cell walls. Bulletin Institute Pasteur 85:287–300
    [Google Scholar]
  10. Fiedler F., Seger J., Schrettenbrunner A., Seeliger H. P. R. 1984; The biochemistry of murein and cell wall teichoic acids in the genus Listeria. Systematic and Applied Microbiology 5:360–376
    [Google Scholar]
  11. Flamm R. K., Hinrichs D. J., Thomashow M. F. 1989; Cloning of a gene encoding a major secreted polypeptide of Listeria monocytogenes and its potential use as a species-specific probe. Applied and Environmental Microbiology 55:2251–2256
    [Google Scholar]
  12. Forsgren A., Forsum U. 1970; Role of protein A in nonspecific immunofluorescence of Staphylococcus aureus. Infection and Immunity 2:387–391
    [Google Scholar]
  13. Gaillard J. L., Berche P., Sansonetti P. 1986; Transposon mutagensis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infection and Immunity 52:50–55
    [Google Scholar]
  14. Gaillard J. L., Berche P., Frehel C., Gouin E., Cossart P. 1991; Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci. Cell 65:1127–1141
    [Google Scholar]
  15. Hof H. 1990; Listerien - eine Herausforderung für die Diagnostic. Immunität und Infektion 18:35–39
    [Google Scholar]
  16. Kathariou S., Metz P., Hof H., Goebel W. 1987; Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes. Journal of Bacteriology 169:1291–1297
    [Google Scholar]
  17. Kocks C., Gouin E., Tabouret M., Berche P., Ohayon H., Cossart P. 1992; L. monocytogenes-induced actin assembly requires the actA gene product,a surface protein. Cell 68:521–531
    [Google Scholar]
  18. Köhler S., Leimeister-Wächter M., Chakraborty T., Lottspeich F., Goebel W. 1990; The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes. Infection and Immunity 58:1943–1950
    [Google Scholar]
  19. Köhler S., Bubert A., Vogel M., Goebel W. 1991; Expression of the iap gene coding for protein p60 of Listeria monocytogenes is controlled on the posttranscriptional level. Journal of Bacteriology 173:4668–4674
    [Google Scholar]
  20. Kuhn M., Goebel W. 1989; Identification of an extracellular protein of Listeria monocytogenes possibly involved in intracellular uptake by mammalian cells. Infection and Immunity 57:55–61
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  22. Leimeister-Wächter M., Domann E., Chakraborty T. 1992; The expression of virulence genes in Listeria monocytogenes is thermoregulated. Journal of Bacteriology 174:947–952
    [Google Scholar]
  23. Mclauchlin J., Ridley A. M., Taylor A. G. 1989; The use of monoclonal antibodies against Listeria monocytogenes in a direct immunofluorescence technique for the rapid presumptive identification and direct demonstration of Listeria in food. Acta Microbiologica Hungarica 36:467–471
    [Google Scholar]
  24. Ruhland G. J., Fiedler F. 1987; Occurrence and biochemistry of lipoteichoic acids in the genus Listeria. Systematic and Applied Microbiology 9:40–46
    [Google Scholar]
  25. Siragusa G. R., Johnson M. G. 1990; Monoclonal antibody specific for Listeria monocytogenes. Listeria innocua, and Listeria welshimeri. Applied and Environmental Microbiology 56:1897–1904
    [Google Scholar]
  26. Tabouret M., De Rycke J., Dubray G. 1992; Analysis of surface proteins of Listeria in relation to species, serovar and pathogenicity. Journal of General Microbiology 138:743–753
    [Google Scholar]
  27. Tilney L. G., Portnoy D. A. 1989; Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. Journal of Cell Biology 109:1597–1608
    [Google Scholar]
  28. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  29. Walker P. D., Batty I., Thomson R. O. 1971; The localization of bacterial antigens by the use of the fluorescent and ferritin labelled antibody techniques. Methods in Microbiology 5A:219–254
    [Google Scholar]
  30. Wang R. F., Cao W. -W., Johnson M. G. 1991; Development of a 16S rRNA-based oligomer probe specific for Listeria monocytogenes. Applied and Environmental Microbiology 57:3666–3670
    [Google Scholar]
  31. Wanner G., Formanek H., Galli D., Wirth R. 1989; Localization of aggregation substances of Enterococcus faecalis after induction by sex pheromones. Archives of Microbiology 151:491–497
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-3-609
Loading
/content/journal/micro/10.1099/00221287-139-3-609
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error