%0 Journal Article %A Vachhani, Ajay K. %A Iyer, Ramkumar K. %A Tuli, Rakesh %T A Mobilizable Shuttle Vector for the Cyanobacterium Plectonema Boryanum %D 1993 %J Microbiology, %V 139 %N 3 %P 569-573 %@ 1465-2080 %R https://doi.org/10.1099/00221287-139-3-569 %I Microbiology Society, %X Plasmid pSUP5011 contains a pMB1 origin of replication, an origin of transfer (oriT), and genes encoding resistance to the antibiotics ampicillin, chloramphenicol and kanamycin. pSUP5011 was conjugally mobilized from Escherichia coli into the non-heterocystous, filamentous, nitrogen-fixing cyanobacterium Plectonema boryanum UTEX 594 in the presence of a helper plasmid, RP4. Transconjugant cyanobacteria selected for resistance to kanamycin, ampicillin and chloramphenicol showed a variety of DNA rearrangements in pSUP5011. One such plasmid continued to show characteristic rearrangements following subsequent transfers into the cyanobacterium. A stable plasmid useful as a shuttle vector was isolated. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-3-569