@article{mbs:/content/journal/micro/10.1099/00221287-139-3-569, author = "Vachhani, Ajay K. and Iyer, Ramkumar K. and Tuli, Rakesh", title = "A Mobilizable Shuttle Vector for the Cyanobacterium Plectonema Boryanum", journal= "Microbiology", year = "1993", volume = "139", number = "3", pages = "569-573", doi = "https://doi.org/10.1099/00221287-139-3-569", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-3-569", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Plasmid pSUP5011 contains a pMB1 origin of replication, an origin of transfer (oriT), and genes encoding resistance to the antibiotics ampicillin, chloramphenicol and kanamycin. pSUP5011 was conjugally mobilized from Escherichia coli into the non-heterocystous, filamentous, nitrogen-fixing cyanobacterium Plectonema boryanum UTEX 594 in the presence of a helper plasmid, RP4. Transconjugant cyanobacteria selected for resistance to kanamycin, ampicillin and chloramphenicol showed a variety of DNA rearrangements in pSUP5011. One such plasmid continued to show characteristic rearrangements following subsequent transfers into the cyanobacterium. A stable plasmid useful as a shuttle vector was isolated.", }