1887

Abstract

Summary: Plasmid pSUP5011 contains a pMB1 origin of replication, an origin of transfer (), and genes encoding resistance to the antibiotics ampicillin, chloramphenicol and kanamycin. pSUP5011 was conjugally mobilized from into the non-heterocystous, filamentous, nitrogen-fixing cyanobacterium UTEX 594 in the presence of a helper plasmid, RP4. Transconjugant cyanobacteria selected for resistance to kanamycin, ampicillin and chloramphenicol showed a variety of DNA rearrangements in pSUP5011. One such plasmid continued to show characteristic rearrangements following subsequent transfers into the cyanobacterium. A stable plasmid useful as a shuttle vector was isolated.

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/content/journal/micro/10.1099/00221287-139-3-569
1993-03-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-3-569
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