1887

Abstract

Exponential phase cells of treated with the superoxide free-radical generating agent menadione (MD; 0·2 m) for 60 min adapted to become resistant to the lethal effects of a higher concentration of MD (4 m). Inhibition of protein synthesis by treatment with cycloheximide totally prevented the adaptation to MD, indicating that this is an inducible response completely dependent on protein synthesis; this differs from the situation with peroxide in which only some of the adaptive response is cycloheximide-sensitive. Cells subjected to heat shock (23 to 37 °C) or treatment with hydrogen peroxide (HO; 0·2 m, 60 min) became more resistant to 4 m-MD; however, MD pretreatment did not induce any thermotolerance or resistance to peroxide. These differences between the response to MD and HO were reflected in the results of -[S]methionine labelling studies. Using one-dimensional electrophoresis, only one polypeptide (60 kDa) was seen to be induced by 0·2 mM–MD and this was also induced by heat shock but not peroxide stress. With heat shock or peroxide treatment the induction of at least 10 polypeptides was detected using this approach. Using an isogenic petite strain, it was found that functional mitochondria were needed for conferring full resistance to MD, but that induction of the adaptive response was not dependent on mitochondrial function.

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1993-03-01
2024-10-06
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References

  1. Andrew P. W., Jackett P. S., Lowrie D. B. 1985; Killing and degradation of microorganisms by macrophages. In Mononuclear Phagocytes: Physiology and Pathology pp. 311–335 Dean R. T., Jessup W. Edited by Amsterdam: Elsevier.;
    [Google Scholar]
  2. Chaput M., Sels A. 1987; Sensitivity of yeast cells to reactive oxygen species generated in the extracellular space. Biochimie 69:53–62
    [Google Scholar]
  3. Christman M. F., Morgan R. W., Jacobson F. S., Ames B. N. 1985; Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium. Cell 41:753–762
    [Google Scholar]
  4. Collinson L. P., Dawes I. W. 1992; Inducibility of the response of yeast cells to peroxide stress. Journal of General Microbiology 138:329–335
    [Google Scholar]
  5. Dean R., Simpson J. 1989; Free radicals: the good, the bad.... Today’s Life Science 1:28–34
    [Google Scholar]
  6. Demple B., Halbrook J. 1983; Inducible repair of oxidative DNA damage in Escherichia coli. Nature; London: 304466–468
    [Google Scholar]
  7. Farr S. B., Kogoma T. 1991; Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiological Review 55:561–585
    [Google Scholar]
  8. Fridovich I. 1983; Superoxide radical: an endogenous radical. Annual Review of Pharmacology and Toxicology 23:239–257
    [Google Scholar]
  9. Greenberg J. T., Demple B. 1989; A global response induced in Escherichia coli by redox-cycling agents overlaps with that induced by peroxide stress. Journal of Bacteriology 171:3933–3939
    [Google Scholar]
  10. Halliwell B., Gutteridge J. M. C. 1989 Free Radicals in Biology and Medicine pp. 161–164, 2nd edn.. Oxford: Clarendon.;
    [Google Scholar]
  11. Hassan H. M., Fridovich I. 1979; Intracellular production of superoxide radical and of hydrogen peroxide by redox active compounds. Archives of Biochemistry and Biophysic 196:385–395
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  13. Nutter L. M., Cheng A.-L., Hung H.-L., Hsieh R.-K., Ngo E. O., Liu T.-W. 1991; Menadione: spectrum of anticancer activity and effects on nucleotide metabolism in human neoplastic cell lines. Biochemical Pharmacology 41:1283–1292
    [Google Scholar]
  14. Nutter L. M., Ngo E. O., Fisher G. R., Gutierrez P. L. 1992; DNA strand scission and free radical production in menadione treated cells. Journal of Biological Chemistry 267:2474–2479
    [Google Scholar]
  15. Phillips J. P., Hilliker A. J. 1990; Genetic analysis of oxygen defense mechanisms in Drosophila melanogaster. Advances in Genetics 28:43–64
    [Google Scholar]
  16. Pitsillides A. A., Blake S. M., Glynn L. E., Bitensky L., Chayen J. 1990; Amelioration by menadione of the experimental chronic immune arthritis in the rabbit. Cell Biochemistry and Function 8:221–226
    [Google Scholar]
  17. Privalle C. T., Fridovich I. 1987; Induction of superoxide dismutase in Escherichia coli by heat shock. Proceedings of the National Academy of Sciences of the United States of America 84:2723–2726
    [Google Scholar]
  18. Scandalios J. G. 1990; Response of plant antioxidant defense genes. Advances in Genetic 28:2–35
    [Google Scholar]
  19. Schenberg-frascino A., Moustacchi E. 1972; Lethal and mutagenic effects of elevated temperature on haploid yeast. Molecular and General Genetic 115:243–257
    [Google Scholar]
  20. Spencer J. F. T., Spencer D. M. 1988; Yeast Genetics. In Yeast: a Practical Approach pp. 65–106 Campbell I., Duffus J. H. Edited by Oxford & Washington: IRL Press.;
    [Google Scholar]
  21. Steels E. L., Learmonth R. P., Watson K. 1991; Heat and oxidative stress as a function of membrane lipid composition in yeast. Proceedings of the Australian Society for Biochemistry and Molecular Biology 23:C46
    [Google Scholar]
  22. Storz G., Christman M. F., Sies H., Ames B. N. 1987; Spontaneous mutagenesis and oxidative damage to DNA in Salmonella typhimurium. Proceedings of the National Academy of Sciences of the United States of America 84:8917–8921
    [Google Scholar]
  23. Storz G., Tartaglia L. A., Ames B. N. 1990; Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation. Science 248:189–194
    [Google Scholar]
  24. Tsaneva I. R., Weiss B. 1990; soxR, a locus governing a superoxide response regulon in Escherichia coli K-12. Journal of Bacteriology 172:4197–4205
    [Google Scholar]
  25. Wu J., Weiss B. 1991; Two divergently transcribed genes,soxR and soxS, control a superoxide response regulon in Escherichia coli. Journal of Bacteriology 173:2864–2871
    [Google Scholar]
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