1887

Abstract

The yeast strain 412 was found to liberate a killer toxin (KT412) lethal to sensitive strains of and . Culture supernatants of the killer strain were concentrated by ultrafiltration and the extracellular protein was purified by gel filtration and ion-exchange chromatography. Gel filtration and SDS-PAGE of the electrophoretically homogeneous killer protein indicated an apparent molecular mass of 10 kDa. The killer toxin KT412 is probably not glycosylated since it did not show any detectable carbohydrate structures. KT412 was bound to sensitive but not to resistant yeast cells. The mannan, and not the glucan, fraction of the cell wall of the sensitive yeast was the primary target for the killer toxin binding. The killer strain 412 contained three double-stranded RNA plasmids of 1·9, 2·9 and 4·0 kb. Curing by cycloheximide resulted in the concomitant loss of killer activity and the 1·9 kb dsRNA species that is therefore regarded as equivalent to the killer-toxin-coding M-plasmids of .

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/content/journal/micro/10.1099/00221287-139-3-495
1993-03-01
2024-03-28
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References

  1. Bevan E. A., Makower M. 1963; The physiological basis of the killer character in yeast. Proceedings of the XI International Congress of Genetic 1:202–203
    [Google Scholar]
  2. Bussey H. 1981; Physiology of killer factor in yeast. Advances in Microbial Physiology 22:93–122
    [Google Scholar]
  3. Bussey H., Boone C., Zhu H., Vernet T., Whiteway M., Thomas D. Y. 1990; Genetic and molecular approaches to synthesis and action of yeast killer toxin. Experientia 46:193–200
    [Google Scholar]
  4. Fink G. R., Styles C. A. 1972; Curing of a killer factor in Saccharomyces cerevisiae. Proceedings of the National Academy of Science of the United States of America 69:2846–2849
    [Google Scholar]
  5. Hutchins K. A., Bussey H. 1983; Cell wall receptor for yeast killer toxin: involvement of (1 → 6)β-d-glucan. Journal of Bacteriology 154:161–168
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 277680–685
    [Google Scholar]
  7. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  8. Martinac B., Zhu H., Kubalski A., Zhou X., Culbertson M., Bussey H., Kung C. 1990; Yeast K1 killer toxin forms ion channels in sensitive yeast spheroplasts and in artificial liposomes. Cell Biology 87:6228–6232
    [Google Scholar]
  9. Middelbeek E. J., Von de Laar H. H. A. M., Hermans J. M. H., Stumm G., Vogels G. D. 1980; Physiological conditions affecting the stability of Saccharomyces cerevisiae killer toxin and energy requirement for toxin action. Antonie van Leeuwenhoek 46:483–497
    [Google Scholar]
  10. Mitchell D. J., Bevan E. A., Herring A. J. 1973; The correlation between dsRNA in yeast and the killer character. Heredity 31:133–134
    [Google Scholar]
  11. Pfeiffer P., Radler F. 1982; Purification and characterization of extracellular and intracellular killer toxin of Saccharomyces cerevisiae strain 28. Journal of General Microbiology 128:2699–2706
    [Google Scholar]
  12. Pfeiffer P., Radler F. 1984; Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2. Archives of Microbiology 137:357–361
    [Google Scholar]
  13. Radler F., Schmitt M. J. 1987; Killer toxins of yeasts: inhibitors of fermentation and their adsorption. Journal of Food Protection 50:234–238
    [Google Scholar]
  14. Radler F., Pfeiffer P., Dennert M. 1985; Killer toxins in new isolates of the yeasts Hanseniaspora uvarum and Pichia kluyveri. FEMS Microbiology Letter 29:269–272
    [Google Scholar]
  15. Schmitt M. J., Pfeiffer P. C. 1990; Immunochemical analysis of the carbohydrate moiety of yeast killer toxin K28. Antonie van Leeuwenhoek 58:277–282
    [Google Scholar]
  16. Schmitt M., Radler F. 1987; Mannoprotein of the yeast cell wall as primary receptor for the killer toxin of Saccharomyces cerevisiae strain 28. Journal of General Microbiology 133:3347–3354
    [Google Scholar]
  17. Schmitt M., Radler F. 1988; Molecular structure of the cell wall receptor for killer toxin KT28 in Saccharomyces cerevisiae. Journal of Bacteriology 170:2192–2196
    [Google Scholar]
  18. Schmitt M. J., Tipper D. J. 1990; K28, a unique double-stranded RNA killer virus of Saccharomyces cerevisiae. Molecular and Cellular Biology 10:4807–4815
    [Google Scholar]
  19. Schmitt M. J., Brendel M., Schwarz R., Radler F. 1989; Inhibition of DNA synthesis in Saccharomyces cerevisiae by yeast killer toxin KT28. Journal of General Microbiology 135:1529–1535
    [Google Scholar]
  20. Tipper D. J., Bostian K. A. 1984; Double-stranded ribonucleic acid killer systems in yeasts. Microbiological Review 48:125–156
    [Google Scholar]
  21. Wickner R. B. 1974; Chromosomal and nonchromosomal mutations affecting the killer character of Saccharomyces cerevisiae. Genetic 76:423–432
    [Google Scholar]
  22. Wickner R. B. 1986; Double-stranded RNA replication in yeast: the killer system. Annual Reviews in Biochemistry 55:373–395
    [Google Scholar]
  23. Young T. W. 1987; Killer yeasts. In The Yeasts pp. 131–164 A. H. Rose, J. S. Harrison. Edited by New York: Academic Press.;
    [Google Scholar]
  24. Young T. W., Philliskirk G. 1977; The production of a yeast killer factor in the chemostat and the effects of killer yeasts in mixed continuous culture with a sensitive strain. Journal of Applied Bacteriology 43:425–436
    [Google Scholar]
  25. Young T. W., Yagiu M. 1978; A comparison of the killer character in different yeasts and its classification. Antonie van Leeuwenhoek 44:59–77
    [Google Scholar]
  26. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. 1969; Glycoprotein staining following electrophoresis on acrylamide gels. Journal of Analyical Biochemistry 30:148–152
    [Google Scholar]
  27. Zorg J., Kilian S., Radler F. 1988; Killer toxin producing strains of the yeasts Hanseniaspora uvarum and Pichia kluyveri. Archives of Microbiology 149:261–267
    [Google Scholar]
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