Purification and Partial Characterization of Acid Phosphatase from Free

Abstract

Non-specific acid phosphatase from cells was purified 111-fold by chromatography on DEAE-cellulose and gel filtration on Sephadex G-100 and Sepharose 4B. The enzyme is a glycoprotein containing 67% neutral sugars. The molecular mass of the highly purified acid phosphatase was found to be approximately 95 kDa by both SDS-PAGE and gel filtration. The pH and temperature optima were 5·8 and 55 °C, respectively. The enzyme was stable at pH values between 3·5 and 5·5 and at temperatures up to 60 °C. The purified phosphatase had a value of 364 m for -nitrophenyl phosphate and showed broad substrate specificity.

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1993-03-01
2024-03-29
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References

  1. Arnold W. N. 1981; Enzymes. pp. 1–46 Arnold W. N. Edited by In Yeast Cell Envelopes: Biochemistry, Biophysics and Ultrastructure II Boca Ration: CRC Press.;
    [Google Scholar]
  2. Barbaric S., Kozulic B., Ries B., Mildner P. 1984; Physicochemical and kinetic properties of acid phosphatase from Saccharomyces cerevisiae. Journal of Biological Chemistry 259:878–883
    [Google Scholar]
  3. Dibenedetto G., Cozzani I. 1975; Nonspecific acid phosphatase from Schizosaccharomyces pombe. Purification and physical chemical properties. Biochemistry 14:2847–2852
    [Google Scholar]
  4. Dibenedetto G., Teller D. C. 1981; Molecular properties and active form of nonspecific acid phosphatase from Schizo-saccharomyces pombe. Journal of Biological Chemistry 256:3926–3930
    [Google Scholar]
  5. Dubois N. K., Gilles K., Hamilton J. L., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. Field C., Schekman R. 1980; Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae. Journal of Cell Biology 86:123–128
    [Google Scholar]
  7. Lanzeta P. A., Alvarez L. J., Reinach P. S., Candia O. A. 1979; An improved assay for nanomole amounts of inorganic phosphate. Analytical Biochemistry 100:95–97
    [Google Scholar]
  8. Linnemans W. A. M., Boer P., Elbers P. F. 1977; Localization of acid phosphatase in Saccharomyces cerevisiae:a clue to cell wall formation. Journal of Bacteriology 131:638–644
    [Google Scholar]
  9. Lopez M. C., Dominguez A. 1988; Purification and properties of a glycoprotein acid phosphatase from the yeast form of Yarrowia lipolytica. Journal of Basic Microbiology 28:249–263
    [Google Scholar]
  10. Odds F. C., Hierholzer J. C. 1973; Purification and properties of a glycoprotein acid phosphatase from Candida albicans. Journal of Bacteriology 144:257–266
    [Google Scholar]
  11. Ogata K., Song S., Yamada H. 1971; Studies on extracellular ribonuclease from yeast. Part I. Purification and physico-chemical properties of RNase from Rhodotorula glutinis. Agricultural and Biological Chemistry 35:58–64
    [Google Scholar]
  12. Onishi M. R., Tkacz J. S., Lampen J. O. 1979; Glycoprotein nature of yeast alkaline phosphatase formation of active enzyme in the presence of tunicamycin. Journal of Biological Chemistry 254:11943–11952
    [Google Scholar]
  13. Schurr A., Yagil E. 1971; Regulation and characterization of acid and alkaline phosphatase in yeast. Journal of General Micro-biology 65:291–303
    [Google Scholar]
  14. Schweingruber A.-M., Schweingruber M. E. 1982; Purification and identification of inactive forms of repressible and constitutive acid phosphatase in yeast. Biochimica et Biophysica Acta 717:203–209
    [Google Scholar]
  15. Trimble R. B., Maley F., Watorek W. 1981; Subunit structure and carbohydrate composition of the extracellular acid phosphatase of Rhodotorula glutinis. Journal of Biological Chemistry 256:10037–10043
    [Google Scholar]
  16. Vasileva-Tonkova E., Petkov P. 1988; Purification of extracellular ribonuclease from Candida lipolytica. Acta Micro-biologica Bulgarica 23:40–48
    [Google Scholar]
  17. Watorek W., Morawiecka B., Korczak B. 1977; Acid phosphatase of the yeast Rhodotorula rubra. Purification and properties of the enzyme. Acta Biochimica Polonica 24:153–162
    [Google Scholar]
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