%0 Journal Article %A Hipkin, Charles R. %A Kau, David A. %A Cannons, Andrew C. %T Further Characterization of the Assimilatory Nitrate Reductase from the Yeast Candida Nitratophila %D 1993 %J Microbiology, %V 139 %N 3 %P 473-478 %@ 1465-2080 %R https://doi.org/10.1099/00221287-139-3-473 %I Microbiology Society, %X Nitrate reductase from the yeast Candida nitratophila was found to contain one molecule of cytochrome b 557 and one atom of molybdenum per subunit. FAD/haem-dependent diaphorase activity (haem domain) was associated with a 40 kDa tryptic fragment of the subunit. The 50 amino-terminal residues of this fragment were determined, and the sequence did not show significant similarity to deduced sequences of other nitrate reductases previously published. Increasing ionic strength in vitro had a stimulatory effect on enzymic activity via stimulation of the molybdenum-dependent terminal nitrate-reducing activity. Stimulation of activity by exogenous protein (bovine serum albumin or casein) also appeared to be an ionic effect. Stimulation of catalytic activity by phosphate was a separate effect. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-3-473