1887

Abstract

Nitrate reductase from the yeast was found to contain one molecule of cytochrome and one atom of molybdenum per subunit. FAD/haem-dependent diaphorase activity (haem domain) was associated with a 40 kDa tryptic fragment of the subunit. The 50 amino-terminal residues of this fragment were determined, and the sequence did not show significant similarity to deduced sequences of other nitrate reductases previously published. Increasing ionic strength had a stimulatory effect on enzymic activity via stimulation of the molybdenum-dependent terminal nitrate-reducing activity. Stimulation of activity by exogenous protein (bovine serum albumin or casein) also appeared to be an ionic effect. Stimulation of catalytic activity by phosphate was a separate effect.

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1993-03-01
2021-07-31
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References

  1. Barber M. J., Notton B. A. 1990; Spinach nitrate reductase. Effects of ionic strength and pH on the full and partial enzyme activities. Plant Physiology 93:537–540
    [Google Scholar]
  2. Campbell W. H., Kinghorn J. R. 1990; Functional domains of assimilatory nitrate reductases and nitrite reductases. Trends in Biochemical Science 15:315–319
    [Google Scholar]
  3. Cannons A. C., Ali A. H., Hipkin C. R. 1986; Regulation of nitrate reductase in the yeast Candida nitratophila. Journal of General Microbiology 132:2005–2011
    [Google Scholar]
  4. De la Rosa M. A., Vega J. M., Zumft W. G. 1981; Composition and structure of assimilatory nitrate reductase from Ankistrodesmus braunii. Journal of Biological Chemistry 256:5814–5819
    [Google Scholar]
  5. Everest S. A., Hipkin C. R., Syrett P. J. 1984; The effect of phosphate and flavin adenine dinucleotide on nitrate reductase activity of some unicellular marine algae. Journal of Experimental Marine Biology and Ecology 76:263–275
    [Google Scholar]
  6. Giri L., Ramadoss C. S. 1979; Physical studies on assimilatory nitrate reductase from Chlorella vulgaris. Journal of Biological Chemistry 254:1703–1712
    [Google Scholar]
  7. Hipkin C. R. 1989; Nitrate assimilation in yeasts. pp. 51–68 Wray J. L., Kinghorn J. R. Edited by In Molecular and Genetic Aspects of Nitrate Assimilation Oxford: Oxford Science Publications.;
    [Google Scholar]
  8. Hipkin C. R., Ali A. H., Cannons A. C. 1986; Structure and properties of assimilatory nitrate reductase from the yeastCandida nitratophila. Journal of General Microbiology 132:1997–2003
    [Google Scholar]
  9. Hipkin C. R., Kau D. A., Cannons A. C., Jones D. H., Gallon J. R., Kay C. J., Barber M. J., Solomonson L. P. 1989; Physical and chemical properties of nitrate reductase from Candida nitratophila. Biochemical Society Transactions 17:928–929
    [Google Scholar]
  10. Horner R. D. 1983; Purification and comparison of nit-1 and wild-type NADPH-nitrate reductase of Neurospora crassa. Biochimica et Biophysica Acta 744:7–15
    [Google Scholar]
  11. Johnstone I. L., McCabe P. C., Greaves P., Cole G. E., Brow M. A. D., Gurr S. J., Unkles S. E., Clutterbuck A. J., Kinghorn J. R., Innis M. 1990; The isolation and characterization of the crnA-niiA-niaD gene cluster for nitrate assimilation in the filamentous fungus Aspergillus nidulans. Gene 90:181–192
    [Google Scholar]
  12. Kay C. J., Barber M. J. 1986; Assimilatory nitrate reductase from Chlorella: effect of ionic strength and pH on catalytic activity. Journal of Biological Chemistry 261:12725–12730
    [Google Scholar]
  13. Kay C. J., Barber M. J., Solomonson L. P., Kau D. A., Cannons A. C., Hipkin C. R. 1990; Spectroscopic, thermodynamic and kinetic properties of Candida nitratophila nitrate reductase. Biochemical Journal 212:545–548
    [Google Scholar]
  14. Kuo T., Kleinhofs A., Warner R. L. 1980; Purification and partial characterisation of nitrate reductase from barley leaves. Plant Science Letters 17:371–381
    [Google Scholar]
  15. Laursen R. A. 1971; Solid phase Edman degradation. An automated peptide sequencer. European Journal of Biochemistry 20:89–102
    [Google Scholar]
  16. Le K. H. D., Lederer F. 1982; On the presence of a haem-binding domain homologous to cytochrome b5 in Neurospora crassa assimilatory nitrate reductase. EMBO Journal 2:1909–1914
    [Google Scholar]
  17. Nicholas D. J. D., Scawin J. H. 1956; A phosphate requirement for nitrate reductase from Neurospora crassa. Nature; London: 1781474–1475
    [Google Scholar]
  18. Okamoto P. M., Fu Y. -H., Marzluf G. A. 1991; nit-3 the structural gene of nitrate reductase in Neurospora crassa: nucleotide sequence and regulation of mRNA synthesis and turnover. Molecular and General Genetics 227:213–223
    [Google Scholar]
  19. Purvis A. C., Tischler C. R., Fites R. C. 1976; In vitro studies of nitrate reductase activity in cotton cotyledons. Plant Physiology 58:95–99
    [Google Scholar]
  20. Redinbaugh M. G., Campbell W. H. 1985; Quaternary structure of squash NADH: nitrate reductase. Journal of Biological Chemistry 260:3380–3385
    [Google Scholar]
  21. Schrader L. E., Cataldo D. A., Peterson D. M. 1974; Use of protein in extraction and stabilization of nitrate reductase. Plant Physiology 53:688–690
    [Google Scholar]
  22. Solomonson L. P., Barber M. J. 1990; Assimilatory nitrate reductase: functional properties and regulation. Annual Review of Plant Physiology 41:225–253
    [Google Scholar]
  23. Solomonson L. P., Lorimer G. H., Hall R. L., Bouchers R., Leggett Bailey J. 1975; Reduced nicotinamide adenine dinucleotide-nitrate reductase of Chlorella vulgaris. Journal of Biological Chemistry 250:4120–4127
    [Google Scholar]
  24. Watcher E., Machleidt W., Hofner H., Otto J. 1973; Amino-propyl glass and its p-phenylene diisothiocyanate derivative, a new support in solid phase Edman degradation of peptides and proteins. FEBS Letters 35:97–102
    [Google Scholar]
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