1887

Abstract

Actinomycin D-resistant RNA synthesis was detected in the cellular (VM) fraction which contains large vesicles plus mitochondria from . It involved the RNAase-resistant complex of naked (unencapsidated) double-stranded RNAs (dsRNAs). The RNA polymerase assay showed [α-P]UTP incorporation in dsRNAs of a size corresponding to the previously described naked M-dsRNAs and in single-stranded RNAs of related size, which are assumed to act as intermediaries in the replicative cycle of the dsRNA molecules. Moreover, the incorporation of ribonucleotides into large mitochondrial dsDNA fragments was detected in the dsRNA-containing strain. This result favours a mitochondrial location for the naked dsRNAs and their associated polymerizing enzyme(s). No replication of the encapsidated large L-dsRNA viral genome was detected in the VM fraction, supporting the hypothesis that the two types of dsRNAs observed in this strain are independent entities.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-2-287
1993-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/2/mic-139-2-287.html?itemId=/content/journal/micro/10.1099/00221287-139-2-287&mimeType=html&fmt=ahah

References

  1. Anagnostakis S.L. 1982; Biological control of chestnut blight. Science 215:466–471
    [Google Scholar]
  2. Barroso G., Labarère J. 1990; Evidence for viral and naked double-stranded RNAs in the basidiomyceteAgrocybe aegerita. Current Genetics 18:231–237
    [Google Scholar]
  3. Ben-Tzvi B.S., Koltin Y., Mevarech M., Tamarkin A. 1984; RNA polymerase activity in virions fromUstilago maydis. Molecular and Cellular Biology 4:188–194
    [Google Scholar]
  4. Buck K.W. 1975; Replication of double-stranded RNA in particles ofPenicillium stoloniferum virus S. Nucleic Acids Research 2:1889–1902
    [Google Scholar]
  5. Buck K.W. 1986; Fungal virology-an overview.. In Fungal Virology pp. 1–84 Buck K.W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  6. Finnegan P.M., Brown G.G. 1986; Autonomously replicating RNA in mitochondria of maize plants with S-type cytoplasm. Proceedings of the National Academy of Sciences of the United States of America 83:5175–5179
    [Google Scholar]
  7. Francki R.I.B., Fauquet C.M., Knudson D.L., Brown F. 1991; Classification and Nomenclature of Viruses.Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology supplementum 2:450
    [Google Scholar]
  8. Hansen D.R., Van Alfen N.K., Gillies K., Powell W.A. 1985; Naked dsRNA associated with hypovirulence ofEndothia parasitica is packaged in fungal vesicles. Journal of General Virology 66:2605–2614
    [Google Scholar]
  9. Lu B.C., Sakaguchi K. 1984; A unique eukaryotic DNA polymerase that transcribes single- and double-stranded RNA. Genetics 107:s65
    [Google Scholar]
  10. Maniatis T., Fritsch E.F., Sambrook J. 1982 In Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  11. Moulinier T., Barroso G., Labarère J. 1992; The mitochondrial genome of the basidiomyceteAgrocybe aegerita: molecular cloning, physical mapping and gene location. Current Genetics 21:499–505
    [Google Scholar]
  12. Noël T., Ho Huynh T.D., Labarère J. 1991; Genetic variability of the wild incompatibility alleles of the tetrapolar basidiomyceteAgrocybe aegerita. Theoretical and Applied Genetics 81:745–751
    [Google Scholar]
  13. Patterson J.L. 1990; Viruses of protozoan parasites. Experimental Parasitology 70:111–113
    [Google Scholar]
  14. Raper C.A., Raper J.R., Miller R.E. 1972; Genetic analysis of the life cycle ofAgaricus bisporus.Mycologia. 64:108–117
    [Google Scholar]
  15. Rogers H.J., Buck K.W., Brasier C.M. 1987; A mitochondrial target for double-stranded RNA in diseased isolates of the fungus that causes Dutch elm disease. Nature; London: 329558–560
    [Google Scholar]
  16. Seroussi E., Peery T., Ginzberg I., Koltin Y. 1989; Detection of killer-independent dsRNA plasmids inUstilago maydis by a simple and rapid method of extraction of dsRNA. Plasmid 21:216–225
    [Google Scholar]
  17. Shapira R., Choi G.H., Nuss D.L. 1991a; Virus-like genetic organization and expression strategy for a double-stranded RNA genetic element associated with biological control of chestnut blight. EMBO Journal 10:731–739
    [Google Scholar]
  18. Shapira R., Choi G.H., Hillman B.I., Nuss D.L. 1991b; The contribution of defective RNAs to the complexity of viral-encoded double-stranded RNA populations present in hypovirulent strains of the chestnut blight fungusCryphonectria parasitica. EMBO Journal 10:741–746
    [Google Scholar]
  19. Sriskantha A., Wach M.P., Schlagnhaufer B., Romaine C.P. 1986; Synthesis of double-stranded RNA in a virus-enriched fraction from Agaricus bisporus. Journal of Virology 57:1004–1009
    [Google Scholar]
  20. Welsh J.D., Leibowitz M.J., Wickner R.B. 1980; Virion DNA-independent RNA polymerase from Saccharomyces cerevisiae. Nucleic Acids Research 8:2349–2363
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-2-287
Loading
/content/journal/micro/10.1099/00221287-139-2-287
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error