1887

Abstract

Sexual cell fusion occurs between NC4 and HM1, heterothallic strains of . Several cell surface proteins relevant to the process have been identified. One of them, gp138, exists in fusion-competent cells of both NC4 and HM1, and is considered to be more concerned with membrane fusion than gamete recognition. In this study, we raised monoclonal antibodies against gp138 and examined gp138 distribution among strains and species of cellular slime moulds to confirm its importance in sexual cell fusion. All heterothallic and bisexual strains examined were found to possess gp138, while asexual and homothallic strains lacked it. The anti-gp138 monoclonal antibody detected several distinct proteins in homothallic strains and one in an asexual strain. Some of the former proteins appeared together with the increase in binucleated cells. Cells of and did not possess proteins reactive to the monoclonal antibody. These results indicate that gp138 is common among, but restricted to, cross-matable strains of . Our results also support previously published molecular phylogenetic studies which suggest that homothallic and asexual strains of are remote from other strains of but are less distantly related to them than other species are.

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1993-02-01
2024-12-04
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References

  1. Blaskovics J.C., Raper K.B. 1957; Encystment stages of Dictyostelium. Biological Bulletin 113:58–88
    [Google Scholar]
  2. Briscoe D.A, Gooley A.A, Bernstein R.L, McKay G.M., Williams K.L. 1987; Genetic diversity in cellular slime molds: allozyme electrophoresis and a monoclonal antibody reveal cryptic species among Dictyostelium discoideumstrains. Genetics 117:213–220
    [Google Scholar]
  3. Cavender J.C., Raper K.B. 1968; The occurrence and distribution of acrasieae in forests of subtropical and tropical America. American Journal of Botany 55:504–513
    [Google Scholar]
  4. Clark M.A., Francis D., Eisenberg R. 1973; Mating types in cellular slime molds. Biochemical and Biophysical Research Communications 52:672–678
    [Google Scholar]
  5. Erdos G.W., Raper K.B., Vogen L.K. 1973; Mating type and macrocyst formation in Dictyostelium discoideum. Proceedings of the National Academy of Sciences of the United States of America 70:1828–1830
    [Google Scholar]
  6. Erdos G.w., Raper K.B., Vogen L.K. 1975; Sexuality in the cellular slime mold Dictyostelium giganteum. Proceedings of the National Academy of Sciences of the United States of America 72:970–973
    [Google Scholar]
  7. Erdos G.w, Raper K.B., Vogen L.K. 1976; Effects of light and temperature on macrocyst formation in paired mating types of Dictyostelium discoideum. Journal of Bacteriology 128:495–497
    [Google Scholar]
  8. Evans W.B., Hughes J.E., Welker D.L. 1988; The use of DNA probes for taxonomic study of Dictyostelium wild isolates. Genetics 119:561–569
    [Google Scholar]
  9. Filosa M.F., Dengler R.E. 1972; Ultrastructure of macrocyst formation in the cellular slime mold, Dictyostelium mucoroides: Extensive phagocytosis of amoebae by a specialized cell. Developmental Biology 29:1–16
    [Google Scholar]
  10. Francis D. 1975; Macrocyst genetics in Polysphondylium pallidum,a cellular slime mould. Journal of General Microbiology 89:310–318
    [Google Scholar]
  11. Glabe C.G., Hong K., Vacquier V.D. 1991; Fusion of sperm and egg plasma membranes during fertilization. In Membrane Fusion pp. 627–646 Edited by Wilschut J., Hoekstra D. New York: Marcel Dekker;
    [Google Scholar]
  12. Herskowitz I. 1989; A regulatory hierarchy for cell specialization in yeast. Nature; London: 342749–757
    [Google Scholar]
  13. Hirabayashi T. 1981; Two-dimensional gel electrophoresis of chicken skeletal muscle proteins with agarose gels in the first dimension. Analytical Biochemistry 117:443–451
    [Google Scholar]
  14. Kirkpatrick M., Jenkins C.D. 1989; Genetic segregation and the maintenance of sexual reproduction. Nature; London: 339300–301
    [Google Scholar]
  15. Kondrashov A.S. 1988; Deleterious mutations and the evolution of sexual reproduction. Nature; London: 336435–440
    [Google Scholar]
  16. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London:680–685
    [Google Scholar]
  17. Luporini P., Heckmann K. 1990; Cell interactions in sexual phenomena. Zoological Science 7 supplement 89–101
    [Google Scholar]
  18. MacInnes M.A., Francis D. 1974; Meiosis in Dictyostelium mucoroides. Nature; London: 251321–324
    [Google Scholar]
  19. Mizutani A., Hagiwara H., Yanagisawa K. 1990; A killer factor produced by the cellular slime mold Polysphondylium pallidum. Archives of Microbiology 153413–416
    [Google Scholar]
  20. Nickerson A.W., Raper K.B. 1973; Macrocysts in the life cycle of the Dictyosteliaceae.I. Formation of the macrocysts. . American Journal of Botany 60:190–197
    [Google Scholar]
  21. Robson G.E., Williams K.L. 1979; Vegetative incompatibility and the mating-type locus in the cellular slime mold Dictyostelium discoideum. Genetics 93:861–875
    [Google Scholar]
  22. Robson G.E., Williams K.L. 1980; The mating system of the cellular slime mould Dictyostelium discoideum. Current Genetics 1:229–232
    [Google Scholar]
  23. Saga Y., Okada H., Yanagisawa K. 1983; Macrocyst development in Dictyostelium discoideum.II. Mating-type-specific cell fusion and acquisition of fusion-competence. Journal of Cell Science 60:157–168
    [Google Scholar]
  24. Sidhu K.S., Guraya S.S. 1991; Current concepts in gamete receptors for fertilization in mammals. International Review of Cytology 127:253–288
    [Google Scholar]
  25. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development.. In Methods in Cell Physiology II pp. 397–410 Edited by Prescott D. New York:: Academic Press;
    [Google Scholar]
  26. Sussman M., Boschwitz C. 1975; Adhesive properties of cell ghosts derived from Dictyostelium discoideum. . Developmental Biology 44:362–368
    [Google Scholar]
  27. Suzuki K., Yanagisawa K. 1989a; Environmental factors inducing sexual development in Dictyostelium discoideum. The Botanical Magazine, Tokyo 102:53–61
    [Google Scholar]
  28. Suzuki K., Yanagisawa K. 1989b; Identification of the cell surface molecule involved in sexual cell fusion of Dictyostelium discoideum.. Differentiation 40:159–165
    [Google Scholar]
  29. Suzuki K., Yanagisawa K. 1990; Purification and characterization of gpl38, a cell surface glycoprotein involved in the sexual cell fusion of Dictyostelium discoideum. Cell Differentiation and Development 30:35–42
    [Google Scholar]
  30. Suzuki T., Amagai A., Maeda Y. 1992; Cyclic AMP and Ca2+ as regulators of zygote formation in the cellular slime mold Dictyostelium mucoroides.. Differentiation 49:127–132
    [Google Scholar]
  31. Urushihara H. 1992; Sexual development of cellular slime molds. Development Growth and Differentiation 34:1–7
    [Google Scholar]
  32. Urushihara H., Saigo T., Yanagisawa K. 1990; Cell fusion promoting factor common to homothallic and heterothallic mating systems in Dictyostelium discoideum. Development Growth and Differentiation 32:111–116
    [Google Scholar]
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