1887

Abstract

Lipase of 9 was purified from culture supernatant fluid. Two polypeptides (51 and 43 kDa) were detected by SDS-PAGE, of which the 43 kDa polypeptide reacted with anti-lipase serum. The 9 lipase gene () was cloned in and localized to a 2·1 kb sequence by subcloning and transposon mutagenesis. The nucleotide sequence of (2064 nucleotides) was determined and the predicted amino acid sequence of the encoded lipase (77 kDa) identified. A 97 kDa lipase was detected in extracts of harbouring and in post-exponential-phase culture supernatant fluids of 9. Data presented indicate that the lipase behaves anomalously during SDS-PAGE and that a pro-lipase is proteolytically processed in cultures of 9 during growth.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-2-267
1993-02-01
2021-07-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/2/mic-139-2-267.html?itemId=/content/journal/micro/10.1099/00221287-139-2-267&mimeType=html&fmt=ahah

References

  1. Appleyard R.K. 1954; Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K12. Genetics 39:440–452
    [Google Scholar]
  2. Arvidson S.O. 1983; Extracellular enzymes from Staphylococcus aureus. . In Staphylococci and Staphylococcal Infections 2 pp. 745–808 Easmon C.S.F., Adlam C. Edited by London: Academic Press;
    [Google Scholar]
  3. Baird-Parker A.C. 1990; The staphylococci: an introduction. Journal of Applied Bacteriology Symposium Supplement 69S:1S–8S
    [Google Scholar]
  4. Bolivar F., Rodriguez R.L., Greene P.J., Betlach M.C., Heynecker H.L., Boyer H.W., Crosa J.H., Falkow S. 1977; Construction and characterization of new cloning vehicles.II.A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  5. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  6. Brady L., Brzozowski A.M., Derewenda Z.S., Dodson E., Dodson G., Tolloy S., Turkenburg J.P., Christiansen L., Huge-Jensen B., Norskov L., Thim L.q, Menge J. 1990; A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature; London: 343767–770
    [Google Scholar]
  7. Coleman D.C., Foster T.J. 1981; Analysis of the reduction in expression of tetracycline resistance determined by transposon Tn10 in the multicopy state. Molecular and General Genetics 182:171–177
    [Google Scholar]
  8. Dougan G., Sherratt D. 1977; The transposon Tn1 as a probe for studying ColEl structure and function. Molecular and General Genetics 151:151–160
    [Google Scholar]
  9. Gotz F., Popp F., Korn E., Schleifer K.H. 1985; Complete nucleotide sequence of the lipase gene fromStaphylococcus hyicus cloned in Staphylococcus carnosus. Nucleic Acids Research 13:5895–5906
    [Google Scholar]
  10. Higgins D.G., Sharp P.M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151–153
    [Google Scholar]
  11. Ingham E., Holland K.T., Gowland G., Cunliffe W.J. 1981; Partial purification and characterization of lipase (EC 3.1.1.3) from Propionibacterium acnes. Journal of General Microbiology 124:393–401
    [Google Scholar]
  12. Ingham E., Holland K.T., Gowland G., Cunliffe W.J. 1984; Difficulties in producing antibodies to purified Propionibacterium acnes exocellular enzymes. British Journal of Dermatology 110:61–66
    [Google Scholar]
  13. Ingham E., Eady E.A., Holland K.T., Gowland G. 1985; Effects of tampon materials on the in-vitro physiology of a toxic shock syndrome strain ofStaphylococcus aureus. 20:87–95
    [Google Scholar]
  14. Jurgens D., Huser H., Brunner H., Fehrenbach F.J. 1981; Purification and characterization of Staphylococcus aureus lipase. FEMS Microbiology Letters 12:195–199
    [Google Scholar]
  15. Kimura H., Kitamura T., Tsuji M. 1972; Studies on human pancreatic lipase.I.Interconversion between low and high molecular weight forms of human pancreatic lipase. Biochimica et Biophysica Acta 270:307–316
    [Google Scholar]
  16. Kloos W.E. 1990; Systematics and the natural history of staphylococci.1. Journal of Applied Bacteriology Symposium Supplement 69S:25S–37S
    [Google Scholar]
  17. Kloos W.E., Schleifer K.H. 1975; Simplified scheme for routine identification of human Staphylococcus species. Journal of Clinical Microbiology 1:82–88
    [Google Scholar]
  18. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  19. Lee C.Y., Iandolo J.J. 1985; Lysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene. Journal of Bacteriology 166:385–391
    [Google Scholar]
  20. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning.A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Marsh J.L., Erfle M., Wykes E.J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481–485
    [Google Scholar]
  22. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Neu H.C., Heppel L.A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  24. O’Reilly M., De Azavedo J.C.S., Kennedy S., Foster T.J. 1986; Inactivation of the alpha-haemolysin of Staphylococcus aureus 8325-4 by site-directed mutagenesis and studies on the expression of its haemolysins. Microbial Pathogenesis 1:125–138
    [Google Scholar]
  25. O’Toole P.W., Foster T.J. 1986; Molecular cloning and expression of the epidermolytic toxin A gene of Staphylococcus aureus. Microbial Pathogenesis 1:583–594
    [Google Scholar]
  26. Patel A.H., Nowlon P., Weavers E.D., Foster T. 1987; Virulence of protein A-deficient and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allele replacement. Infection and Immunity 55:3103–3110
    [Google Scholar]
  27. Perlman D., Halvorson H.O. 1983; A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. Journal of Molecular Biology 167:391–409
    [Google Scholar]
  28. Phonimdaeng P., O’Reilly M., Nowlan P., Bramley A.J., Foster T.J. 1990; The coagulase of Staphylococcus aureus 8325-4 Sequence analysis and virulence of site-specific coagulase-deficient mutants. Molecular Microbiology 4:393–404
    [Google Scholar]
  29. Pulverer G., Peters G., Schumacher-Perdreau F. 1987; Coagulase-negative staphylococci. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene Series A 264:1–28
    [Google Scholar]
  30. Russell R.R.B., Coleman D., Dougan G. 1985; Expression of a gene for glucan-binding from Streptococcus mutans in Escherichia coli. Journal of General Microbiology 131:295–299
    [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  32. Taylor D., Holland K.T. 1989; Amino acid requirements for the growth and production of some exocellular products of Staphylococcus aureus. Journal of Applied Bacteriology 66:319–329
    [Google Scholar]
  33. Tyski S., Hryniewicz W., Jeljaszewicz J. 1983; Purification and some properties of the staphylococcal extracellular lipase. Biochimica et Biophysica Acta 749:312–317
    [Google Scholar]
  34. Vadehra D.V. 1974; Staphylococcal lipases. Lipids 9:158–165
    [Google Scholar]
  35. Van Oort M.G., Deveer A.M.TH.J, Dijkman R., Tjeenk M.L., Verheij H.M., De Haas G.H., Wenzig E., Gotz F. 1989; Purification and substrate specificity of Staphylococcus hyicus lipase. Biochemistry 28:9278–9285
    [Google Scholar]
  36. Wain-Hobson S.S., Sonigo P., Danos O., Cole S., Alizon M. 1985; Nucleotide sequence of the AIDS virus, LAV. Cell 40:9–17
    [Google Scholar]
  37. Winkler F.K., D’Arcy A., Hunziker W. 1990; Structure of human pancreatic lipase. Nature; London: 343771–774
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-2-267
Loading
/content/journal/micro/10.1099/00221287-139-2-267
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error