1887

Abstract

An insertion sequence (IS) element of named IS was isolated and its complete nucleotide sequence determined. IS is 836 bp in length and occurs 20–35 times in the genome and 5–15 times in other species. Analysis of the junctions at the sites of insertion revealed a small target site duplication of four bases and inverted repeats of 17 bp with one mismatch. presents significant similarity (53·4%) with IS427 identified in suggesting a common ancestral sequence. A long ORF of 708 bp was identified encoding a protein with a predicted molecular mass of 26 kDa and sharing sequence identity with the hypothetical protein 1 of and with the transposase of . IS is present in all strains we have tested. Restriction fragment length polymorphism of reference and field strains of two species and was studied using either pulsed field gel electrophoresis (PFGE) on -digested DNA or hybridization of RI-digested DNA using IS as a probe. The genome of biovar 3 contains about 10 IS copies per genome and field strains of the same species could not be distinguished either by IS hybridization or by I (PFGE) restriction patterns. In contrast, the number of IS copies in the genome is around 30 and the different field strains can be differentiated by both methods. As ISs have been shown to be implicated in chromosomal rearrangement, we propose that the chromosomal polymorphism revealed by PFGE and high copy number of IS observed in may be related to the presence of an active IS in this species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-12-3265
1993-12-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/12/mic-139-12-3265.html?itemId=/content/journal/micro/10.1099/00221287-139-12-3265&mimeType=html&fmt=ahah

References

  1. Allardet-Servent A., Bourg G., Ramuz M., Pages M., Bellis M., Roizes G. 1988; DNA polymorphism in strains of the genus Brucella. Journal of Bacteriology 170:4603–4607
    [Google Scholar]
  2. Allardet-Servent A., Carles-Nurit M.J., Bourg G., Michaux S., Ramuz M. 1991; Physical map of the Brucella melitensis 16M chromosome. Journal of Bacteriology 173:2219–2224
    [Google Scholar]
  3. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. 1987 Current Protocols in Molecular Biology 1 New York: Wiley Interscience;
    [Google Scholar]
  4. Canard B., St-Joanis B., Cole S.T. 1992; Genomic diversity and organization of various genes in the pathogenic anaerobe Clostridium perfringens. Molecular Microbiology 6:1421–1429
    [Google Scholar]
  5. Carlson C.R., Gronsdat A., Kolsto A.B. 1992; Physical maps of the genomes of three Bacillus cereus. Journal of Bacteriology 174:3750–3756
    [Google Scholar]
  6. Cellier M., Teyssier J., Nicolas M., Liautard J.P., Marti J., Sri Widada J. 1992; Cloning and characterization of the Brucella ovis heat shock protein DnaK functionally expressed in E. coli. Journal of Bacteriology 174:8036–8042
    [Google Scholar]
  7. Corbel M.J., Brinley-Morgan W.J. 1984; Genus Brucella Meyer and Shaw 1920 173AL. In Bergey’s Manual of Systematic Bacteriology 1 pp. 377–388 Krieg N.R., Holt J.G. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. De Meirsman C., Van Soom C., Verreth C., Van Gool A., Vanderleyden J. 1990; Nucleotide sequence analysis of IS427 and its target sites in Agrobacterium tumefaciens T37. Plasmid 24:227–234
    [Google Scholar]
  9. Dessen P., Fondrat C., Valencien C., Mugnier C. 1990; bisance: a French service for access to biomolecular sequence data bases. CABIOS 6:335–356
    [Google Scholar]
  10. Fekete A., Bantle J.A., Halling S., Sanborn M.R. 1990a; Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. Journal of Appied Bacteriology 69:216–227
    [Google Scholar]
  11. Fekete A., Bantle J.A., Halling S., Sanborn M.R. 1990b; Rapid, sensitive detection of Brucella abortus by polymerase chain reaction without extraction of DNA. Biotechnology Techniques 4:31–34
    [Google Scholar]
  12. Galas I., Chandler M. 1989; Bacterial insertion elements. In Mobile DNA pp. 109–162 Berg D.E., Howe M.M. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Gamas P., Chandler M., Prentki P., Galas I. 1987; Escherichia coli integration host factor binds specifically to the ends of the insertion sequence IS7 and to its major hot-spot in pBR322. Journal of Molecular Biology 195:261–272
    [Google Scholar]
  14. Garnier J., Osguthorpe A., Robson B. 1978; Analysis of the accurance and implication of simple methods for predicting the secondary structure of globular proteins. Journal of Molecular Biology 120:97–120
    [Google Scholar]
  15. Glare E.M., Paton J.C., Premier R.R., Lawrence A.J., Nisbet I.T. 1990; Analysis of a repetitive DNA sequence from Bordetella pertussis and its appication to the diagnosis of pertussis using the polymerase chain reaction. Journal of Clinical Microbiology 28:1982–1987
    [Google Scholar]
  16. Grindley N.D., Wiater L.A. 1988; gd transposase and integration host factor bind cooperatively at both ends of gd. EMBO Journal 7:1907–1912
    [Google Scholar]
  17. Halling S., Zehr E. 1990; Polymorphism in Brucella spp. due to highly repeated DNA. Journal of Bacteriology 172:6637–6640
    [Google Scholar]
  18. Hermans P.W.M., Van Soolingen D., Bik E.M., Dehaas P.E.W., Dale J.W., Van Embden J.D.A. 1990; Insertion element IS987 from Mycobacterium bovis BCG is located in a hotspot integration region from insertion elements in Mycobacterium tuberculosis complex strains. Infection and Immunity 59:2695–2705
    [Google Scholar]
  19. Iida S., Meyer J., Arber W. 1983; Prokaryotic IS elements. In Mobile Genetic Elements pp. 159–221 Shapiro J.A. Edited by San Diego: Academic Press;
    [Google Scholar]
  20. Mayfield J.E., Bricker B.J., Godfrey H., Crosby R.M., Knight D.J., Halling S.M., Balinsky D., Tabatai L.B. 1988; The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein. Gene 63:1–9
    [Google Scholar]
  21. Meyer M.E., Cameron H.S. 1961; Metabolic characterization of the genus Brucella. I. Statistical evaluation of the oxidative tests by which type 1 of each species can be identified. Journal of Bacteriology 82:387–395
    [Google Scholar]
  22. Michaux S., Paillisson J., Carles-Murit M.J., Bourg G., Allardet-Servent A., Ramuz M. 1993; Presence of two independent chromosomes in the Brucella melitensis16M genome. Journal of Bacteriology 175:701–705
    [Google Scholar]
  23. Mills J.A., Venkatesan M.M., Baron L., Buysse J.M. 1992; Spontaneous insertion of an IS l-like element into the virF gene is responsible for avirulence in opaque colonial variants of Shigella flexneri2a. Infection and Immunity 60:175–182
    [Google Scholar]
  24. Moreno E., Stackebrandt E., Dorsh M., Wolters J., Bush M., Mayer H. 1990; Brucella abortus16S rRNA and Lipid A reveal a phylogenetic relationship with members of the Alpha-2 subdivision of the class Proteobacteria. Journal of Bacteriology 172:3569–3576
    [Google Scholar]
  25. Paulus F., Canady J., Vincent F., Bonnard G., Kares C., Otten L. 1991; Sequence of the iaa and ipt region of different Agrobacterium tumefaciens biotype III octopine strains: reconstruction of octopine Ti plasmid evolution. Plant Molecular Biology 16:601–614
    [Google Scholar]
  26. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Verger J.M., Grimont F., Grimont P.A.D., Grayon M. 1985; Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. International Journal of Systematic Bacteriology 35:292–295
    [Google Scholar]
  28. Weisburg W., Barns S.M., Pelletier D.A., Lane D.L. 1991; 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173:697–703
    [Google Scholar]
  29. Woese C.R., Stackebrandt E., Weisburg W.G., Paster B.J., Madigan M.T., Fowler R.V.J., Hahn C.M., Blanz P., Gupta R., Nealson K.H., Fox G.E. 1984; The phylogeny of purple bacteria: the alpha subdivision. Systematic and Appied Microbiology 5:315–326
    [Google Scholar]
/content/journal/micro/10.1099/00221287-139-12-3265
Loading
/content/journal/micro/10.1099/00221287-139-12-3265
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error