1887

Abstract

SUMMARY: The gene encoding a periplasmic pH 2.5 acid phosphatase from was placed under the control of an inducible promoter and integrated into the chromosome of The majority of the AppA protein synthesized in accumulated in the periplasm whereas about 30% was secreted into the medium. The kinetics of AppA secretion through the ‘outer envelopes’ (i.e. periplasm+outer membrane) were followed after AppA induction. The results suggest that AppA crosses these envelopes by a mechanism involving diffusion and show that the periplasmic accumulation of AppA and envelope permeability are decreased by mutations that decrease the secretion of native proteins in

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/content/journal/micro/10.1099/00221287-139-12-3243
1993-12-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-12-3243
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