Utilization of organosulphur compounds by axenic and mixed cultures of IGTS8 Free

Abstract

Growth assays reveal that IGTS8 uses a wide range of organosulphur compounds as the sole source of sulphur, yet none of the compounds serve as carbon sources. Compounds that are utilized include thiophenes, sulphides, disulphides, mercaptans, sulphoxides, and sulphones. A convenient spectrophotometric assay (Gibbs assay), based on the chromogenic reaction of 2,6-dichloroquinone-4-chloroimide with aromatic hydroxyl groups, was developed and used in conjunction with GC/MS analyses to examine the kinetics of dibenzothiophene metabolism by axenic and mixed cell cultures of IGTS8. The desulphurization trait is expressed at increasing levels during the exponential phase of growth and then declines in stationary-phase cells. Mixtures of streptomycin-resistant IGTS8 and (an organism incapable of cleaving carbon-sulphur bonds in relevant test compounds) were prepared in ratios that varied over six orders of magnitude. Growth studies revealed that was able to gain access to sulphur liberated from organosulphur compounds by IGTS8; however, cell-to-cell contact appears to be required. These experiments also indicate that the desulphurization activity, on a per cell basis, is higher in mixed cultures than in axenic cultures.

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1993-12-01
2024-03-29
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References

  1. Van Afferden M., Schacht S., Klein J., Truper H.G. 1990; Degradation of dibenzothiophene by Brevibacterium sp. DO. Archives of Microbiology 153:324–328
    [Google Scholar]
  2. Gallagher J.R., Olson E.S., Stanley D.C. 1993; Microbial desulfurization of dibenzothiophene: a sulfur specific pathway. FEMS Microbiology Letters 107:31–35
    [Google Scholar]
  3. Hoffman M.R., Faust B.C., Panda F.A., Koo H.H., Tsuchiya H.M. 1981; Kinetics of the removal of iron pyrite from coal by microbial catalysts. Applied and Environmental Microbiology 42:259–271
    [Google Scholar]
  4. Hou C.T., Laskin A.I. 1976; Microbial conversion of dibenzo-thiphene. Developments in Industrial Microbiology 17:351–362
    [Google Scholar]
  5. Isbister J.D., Kobylinski E.A. 1985; Microbial desulfurization of coal. In Processing and Utilization of High Sulfur Coals 9 Coal Science and Technology Series pp. 627–641 Attia Y.A. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  6. Kargi F., Robinson J.M. 1984; Microbial oxidation of dibenzothiophene by the thermophilic organism Sulfulobus acido-caldarius. Biotechnology and Bioengineering 26:687–690
    [Google Scholar]
  7. Kilbane J.J. 1989; Desulfurization of coal: the microbial solution. Trends in Biotechnology 7:97–101
    [Google Scholar]
  8. Kilbane J.J. 1990a; Biodesulfurization of coal. In Gas, Oil, and Coal Biotechnology pp. 33 Akin C., Smith J. Edited by Chicago: Institute of Gas Technology;
    [Google Scholar]
  9. Kilbane J.J. 1990b; Sulfur specific microbial metabolism of organosulfur compound resources. Conservation and Recycling 3:69–79
    [Google Scholar]
  10. Kilbane J.J. 1991; Microbial removal or organic sulfur from coal and petroleum. In Proceedings: 1991 Second International Biological Processing of Coal. Palo Alto, California: Electric Power Research Institute.;
    [Google Scholar]
  11. Kilbane J.J., Jackowski K. 1992; Biodesulfurization of water-soluble coal-derived material by Rhodococcus rhodochrous IGST8. Biotechnology and Bioengineering 40:1107–1114
    [Google Scholar]
  12. Kodama K., Umehara K., Shimizu K., Nakatani S., Minoda Y., Yamada K. 1973; Identification of microbial products from dibenzothiophene and its proposed oxidation pathway. Agricultural and Biological Chemistry 37:45–50
    [Google Scholar]
  13. Laborde A.L., Gibson D.T. 1977; Metabolism of dibenzo-thiphene by a Beijerinkia species. Applied and Environmental Microbiology 24:783–790
    [Google Scholar]
  14. Malick K.A. 1978; Microbial removal of organic sulfur from crude oil and the environment: some new perspectives. Process Biochemistry 13:10–12
    [Google Scholar]
  15. Monticello D.J., Barker D., Finnerty W.R. 1985; Plasmid mediated degradation of dibenzothiphene by Pseudomonas species. Applied and Environmental Microbiology 49:756–760
    [Google Scholar]
  16. Omori T., Mouna L., Saiki Y., Kodama T. 1992; Desulfurization of dibenzothiphene by Corynebacterium sp. strain SY1. Applied and Environmental Microbiology 58:911–915
    [Google Scholar]
  17. Silverman M.P., Rogoff M.H., Wender I. 1963; Removal of pyritic sulfur from coal by bacterial action. Fuel 42:113–124
    [Google Scholar]
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