1887

Abstract

strains causing human extra-intestinal infections may be divided into two groups, B and B according to the electrophoretic patterns of carboxylesterase B. This study compares the restriction fragment length polymorphism (RFLP) of ribosomal DNA (rDNA) for 45 B strains and 45 B strains to examine the genetic structure of B strains and to distinguish them from B strains. The isolates were chosen for diversity in their allozymes of esterases, B, A, C and I, their production of virulence factors (α-haemolysin, mannose resistant haemagglutinin and cytotoxic necrotizing factor) and certain O antigens, and their pathological and geographical origins. DNA was digested with dIII and HI restriction enzymes and analysed by Southern blotting. The resulting rDNA RFLP patterns of B strains were distinct from those of the B strains. Moreover, the B strains appeared to be less heterogeneous than the B strains. The B strains gave 13 ribotypes (resulting from the combination of the rDNA RFLP patterns obtained with dIII and HI digestions) while the B strains gave 32 ribotypes. Correspondence analysis of the data showed that several clusters of strains were identified in the B strains by particular ribotypes, certain associations of esterase B and A electrophoretic variants, O serotypes and virulence factor production. In contrast, these parameters appeared to be unrelated in the B strains, reflecting their heterogeneity. These findings, which differentiate two levels of genetic heterogeneity within pathogenic isolates, indicate that the B strains constitute a phylogenetically distinct group within the species.

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1993-12-01
2021-07-31
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References

  1. Achtman M., Pluschke G. 1986; Clonal analysis of descent and virulence among selected Escherichia coli. Annual Review of Microbiology 40:185–210
    [Google Scholar]
  2. Achtman M., Heuzenroeder M., Kusecek B., Ochman H., Caugant D., Selander R.K., Vaisanen-Rhen V., Korhonen T.K., Stuart S., Orskov F., Orskov I. 1986; Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. Infection and Immunity 51:268–276
    [Google Scholar]
  3. Arbeit R.D., Arthur M., Dunn R., Kim C., Selander R.K., Goldstein R. 1990; Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. Journal of Infectious Diseases 161:230–235
    [Google Scholar]
  4. Arthur M., Arbeit R.D., Kim C., Beltran P., Crowe H., Steinbach S., Campanelli C, Wilson R.A., Selander R.K., Goldstein R. 1990; Restriction fragment length polymorphisms among uropathogenic Escherichia coli isolates: pap-related sequences compared with rrn operons. Infection and Immunity 58:471–479
    [Google Scholar]
  5. Bingen E., Denamur E., Lambert-Zechovsky N., Aujard Y., Brahimi N., Geslin P., Elion J. 1992; Analysis of DNA restriction fragment length polymorphism extends the evidence for breast milk transmission in Streptococcus agalactiae late-onset neonatal infection. Journal of Infectious Diseases 165:569–573
    [Google Scholar]
  6. Caprioli A., Falbo L., Roda G., Ruggeri F.M., Zona C. 1983; Partial purification and characterization of an Escherichia coli toxic factor that induces morphological cell alterations. Infection and Immunity 39:1300–1306
    [Google Scholar]
  7. Caprioli A., Falbo V., Ruggeri F.M., Baldassarri L., Bisicchia R., Ippolito G., Romoli E., Donelli G. 1987; Cytotoxic necrotizing factor production by hemolytic strains of Escherichia coli causing extraintestinal infections. Journal of Clinical Microbiology 25:146–149
    [Google Scholar]
  8. Caugant D.A., Levin B.R., Lidin-Janson G., Whittam T.S., Svanborg-Eden C., Selander R.K. 1983; Genetic diversity and relationships among strains of Escherichia coli in the intestine and those causing urinary tract infections. Progress in Allergy 33:203–227
    [Google Scholar]
  9. Cherifi A., Contrepois M., Picard B., Goullet PH., De Rycke J., Fairbrother J., Barnouin J. 1990; Factors and markers of virulence in Escherichia coli from human septicemia. FEMS Microbiology Letters 70:279–284
    [Google Scholar]
  10. De Rycke J., Guillot J.F., Boivin R. 1987; Cytotoxins in non-enterotoxigenic strains of Escherichia coli isolated from feces of diarrheic calves. Veterinary Microbiology 15:137–150
    [Google Scholar]
  11. De Rycke J., Gonzalez E.A., Blanco J., Oswald E., Blanco M., Boivin R. 1990; Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli. Journal of Clinical Microbiology 28:694–699
    [Google Scholar]
  12. Denamur E., Picard-Pasquier N., Mura C., Picard B., Orfila J., Krishnamoorthy R. 1991; Comparison of molecular epi-demiological tools for Branhamella catarrhalis typing. Research in Microbiology 142:585–589
    [Google Scholar]
  13. Evans D.J., Evans D.G., Fiohne C., Noble M.A., Haldane E.V., Lior H., Young L.S. 1981; Hemolysin and K-antigens in relation to serotype and hemagglutination type of Escherichia coli isolated from extraintestinal infections. Journal of Clinical Microbiology 13:171–178
    [Google Scholar]
  14. Goullet PH. 1973; An esterase zymogram of Escherichia coli. Journal of General Microbiology 77:27–35
    [Google Scholar]
  15. Goullet PH., Picard B. 1986a; Comparative esterase electrophoretic polymorphism of Escherichia coli isolates obtained from animal and human sources. Journal of General Microbiology 132:1843–1851
    [Google Scholar]
  16. Goullet PH., Picard B. 1986b; Highly pathogenic strains of Escherichia coli revealed by the distinct electrophoretic pattern of carboxylesterase B. Journal of General Microbiology 132:1853–1858
    [Google Scholar]
  17. Goullet PH., Picard B. 1989; Comparative electrophoretic polymorphism of esterases and other enzymes in Escherichia coli. Journal of General Microbiology 135:135–143
    [Google Scholar]
  18. Goullet PH., Picard B. 1990; Electrophoretic type B2 of carboxylesterase B for characterisation of highly pathogenic Escherichia coli strains from extra-intestinal infections. Journal of Medical Microbiology 33:11–16
    [Google Scholar]
  19. Goullet PH., Picard B., SevaliGarcia J. 1986; Electrophoretic mobility of an esterase from Escherichia coli isolated from extraintestinal infections. Journal of Infectious Diseases 154:727–728
    [Google Scholar]
  20. Goullet PH., Picard B., Krishnamoorthy R. 1988; An evaluation of allozyme amino-acid substitutions for the study of molecular relationships in Providencia. Annates de I̓Institut Pasteur/Microbiology 139:689–702
    [Google Scholar]
  21. Goullet PH., Picard B., Contrepois M., De Rycke J., Barnouin J. 1993; Correlation between esterase electrophoretic polymorphism and virulence-associated traits in extra-intestinal invasive strains of Escherichia coli. Epidemiology and Infection in the Press
    [Google Scholar]
  22. Greenacre M.J. 1984 Theory and Applications of Correspondence Analysis. London: Academic Press.;
    [Google Scholar]
  23. Grimont F., Grimont P.A.D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Annales de I̓Institut Pasteur/Microbiologie 137B:165–175
    [Google Scholar]
  24. Hacker J., Schroter G., Schrettenbrunner A., Hughes C., Goebel W. 1983; Hemolytic Escherichia coli strains in the human fecal flora as potential urinary pathogens. Zeintralblatt für Bakteriologie, Mikrobiologie und Hygiene 254:370–378
    [Google Scholar]
  25. Herzer P.J., Inouye S., Inouye M., Whittam T. 1990; Phylogenetic distribution of branched RNA-linked multicopy singlestranded DNA among natural isolates of Escherichia coli. Journal of Bacteriology 172:6175–6181
    [Google Scholar]
  26. Johnson J.R. 1991; Virulence factors in Escherichia coli urinary tract infection. Clinical Microbiology Reviews 4:80–128
    [Google Scholar]
  27. Johnson J.R., Goullet PH., Picard B., Moseley S.L., Roberts P.L., Stamm W.E. 1991; Association of carboxylesterase B electrophoretic pattern with presence and expression of urovirulence factor determinants and antimicrobial resistance among strains of Escherichia coli that cause urosepsis. Infection and Immunity 59:2311–2315
    [Google Scholar]
  28. Le Minor S., Le Coueffic E. 1975; Étude sur les hémolysines des Enterobacteriaceae. Annales de Microbiologie 126:313–332
    [Google Scholar]
  29. Lebart L., Morineau A., Warwick K.M. 1984; Multivariate descriptive analysis: correspondence analysis and related techniques for large matrices. New York: Wiley-Interscience.;
    [Google Scholar]
  30. Lerman I.C. 1981 Classification et Analyse ordinate des Données. Paris:: Dunod.;
    [Google Scholar]
  31. Low D., David V., Lark D., Schoolnik G., Falkow S. 1984; Gene clusters governing the production of hemolysin and mannose- resistant hemagglutination are closely linked in Escherichia coli serotypes O4 and O6 isolated from urinary tract infections. Infection and Immunity 43:353–358
    [Google Scholar]
  32. O’Hanley P., Low D., Romero I., Lark K., Vosti K., Falkow S., Schoolnik G. 1985; Gal-Gal binding and hemolysin phenotypes and genotypes associated with uropathogenic Escherichia coli. New England Journal of Medicine 7:414–420
    [Google Scholar]
  33. Orskov I., Orskov F. 1985; Escherichia coli in extra-intestinal infections. Journal of Hygiene 95:551–575
    [Google Scholar]
  34. Picard B., Goullet PH. 1988; Correlation between electrophoretic types B1 and B2 of carboxylesterase B and host-dependent factors in Escherichia coli septicaemia. Epidemiology and Infection 100:51–61
    [Google Scholar]
  35. Picard B., Goullet PH. 1989; Correlation between electrophoretic types B1 and B2 of carboxylesterase B and sex of patients in Escherichia coli urinary tract infections. Epidemiology and Infection 103:97–103
    [Google Scholar]
  36. Picard B., Goullet PH., Krishnamoorthy R. 1987; A novel approach to study of the structural basis of enzyme polymorphism. Analysis of carboxylesterase B of Escherichia coli as model. Biochemical Journal 241:877–881
    [Google Scholar]
  37. Picard B., Goullet PH., Contrepois M. 1988; Clonal distribution of septicaemic Escherichia coli O78 isolates evidenced by enzyme electrophoretic polymorphism. Annales de I̓Institut Pasteur/Microbiology 139:239–242
    [Google Scholar]
  38. Picard B., Picard-Pasquier N., Krishnamoorthy R., Goullet PH. 1991a; Correlation between DNA polymorphism and enzyme polymorphism argues in favour of the delineation of two species within Providencia alcalifaciens. Research in Microbiology 142:965–969
    [Google Scholar]
  39. Picard B., Picard-Pasquier N., Krishnamoorthy R., Goullet PH. 1991b; Characterization of highly virulent Escherichia coli strains by ribosomal DNA restriction fragment length polymorphism. FEMS Microbiology Letters 82:183–188
    [Google Scholar]
  40. Picard-Pasquier N., Ouaghed M., Picard B., Goullet PH., Krishnamoorthy R. 1989; A simple sensitive method for analysing bacterial ribosomal DNA polymorphism. Electrophoresis 10:186–189
    [Google Scholar]
  41. Picard-Pasquier N., Picard B., Heeralal S., Krishnamoorthy R., Goullet PH. 1990; Correlation between ribosomal DNA polymorphism and electrophoretic enzyme polymorphism in Yersinia. Journal of General Microbiology 136:1655–1666
    [Google Scholar]
  42. Plos K., Hull S.I., Hull R.A., Levin B.R., Orskov I., Orskov F., Svanborg-Eden C. 1989; Distribution of the P-associated-pilus (pap) region among Escherichia coli from natural sources: evidence for horizontal gene transfer. Infection and Immunity 57:1604–1611
    [Google Scholar]
  43. Selander R.K., Korhoenen T.K., Vaisanen-Rhen V., Williams P.H., Pattison P.E., Caugant D.A. 1986; Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infection and Immunity 52:213–222
    [Google Scholar]
  44. Selander R.K., Caugant D.A., Whittam T.S. 1987; Genetic structure and variation in natural populations of Escherichia coli. In Escherichia coli and Salmonella typhimurium, Cellular and Molecular Biolog 2 pp. 1625–1648 Ingraham J.L. others Edited by Washington DC:: American Society for Microbiology.;
    [Google Scholar]
  45. Tenenhaus M., Young F.W. 1985; An analysis and synthesis of multiple correspondence analysis, optimal scaling, dual scaling, homogeneity analysis and other methods for quantifying categorical multivariate data. Psychometrika 50:91–119
    [Google Scholar]
  46. Vosti K.L. 1979; Relationship of hemagglutination to other biological properties of serologically classified isolates of Escherichia coli. Infection and Immunity 25:507–512
    [Google Scholar]
  47. Woods T.C., Helsel L.O., Swaminathan B., Bibb W.F., Pinner R.W., Gellin B.G., Collin S.F., Waterman S.H., Reeves M.W., Brenner D.J., Broome C.V. 1992; Characterization of Neisseria meningitidis serogroup C by multilocus enzyme electro-phoresis and ribosomal DNA restriction profiles (ribotyping). Journal of Clinical Microbiology 30:132–137
    [Google Scholar]
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