RT Journal Article SR Electronic(1) A1 Sucic, Joseph F. A1 Luo, Shun A1 Williamson, Brian D. A1 Yin, Yizhong A1 Rogers, Patricia V. A1 Rutherford, Charles L.YR 1993 T1 Developmental and cAMP-mediated regulation of glycogen phosphorylase 1 in Dictyostelium discoideum JF Microbiology, VO 139 IS 12 SP 3043 OP 3052 DO https://doi.org/10.1099/00221287-139-12-3043 PB Microbiology Society, SN 1465-2080, AB The Dictyostelium discoideum glycogen phosphorylase-1 (gp-1) exhibits a complex pattern of developmental expression in which differential temporal regulation of enzyme activity, protein levels and mRNA levels is observed. This pattern of expression implies that gp-1 regulation occurs at multiple levels, probably involving both transcriptional and post-transcriptional events. Post-translational control of gp-1 activity, in effect, actually regulates the protein from a developmental perspective. In this report we have examined several facets of this regulation. We show that addition of exogenous cAMP to cells in suspension culture caused changes in gp-1 enzyme activity and mRNA levels that are identical to those observed during normal development, suggesting that cAMP is involved in the regulation of gp-1. Exogenous cAMP could regulate gp-1 mRNA expression at concentrations as low as 1·0 μm. cAMP regulation of gp-1 mRNA appeared to occur through a mechanism that required intracellular cAMP signalling. We identified regions of the promoter necessary for gp-1 expression by using gp-1 promoter deletions to drive the expression of a luciferase reporter gene. Results of these experiments suggested that developmental and cAMP-mediated changes in gp-1 mRNA levels were the result of alterations in transcription. The promoter analysis also suggested that a vegetative specific element is located between −785 and −1894 nucleotides from the transcriptional start site. Elements necessary for maximal developmental and cAMP-mediated expression appear to be located between −1153 and −1894 nucleotides from the cap site. Sequence elements located between −180 and −1153 appear to be required for a basal level of late developmental expression., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-139-12-3043