1887

Abstract

SUMMARY: For the first time polyphosphate (poly P) granules have been detected in organisms colonizing the gastric antrum as well as in organisms isolated from the same tissue. Poly P granules showed typical sublimation characteristics during exposure to the electron beam and chipped out of ultrathin sectioning. A prominent phosphorus signal was identified using elemental specific electron microscopy such as electron energy loss spectroscopy (EELS) and was localized to at least three different locations: the cytoplasm, the flagellar pole and in association with the cell membrane. Intracytoplasmatic structures had a diameter of 0.05-0.2 μm, whereas the structures near the flagellar pole were much smaller (0.02 μm). The membrane-associated phosphate aggregates were visible only after staining with Pb(NO) or with electron spectroscopic imaging (ESI). Poly P granules seem to be important energy and phosphorus stores and it is thought that they participate in the regulation of various and distinct metabolic processes of

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/content/journal/micro/10.1099/00221287-139-12-3029
1993-12-01
2019-10-19
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-12-3029
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