1887

Abstract

When grown on ethene strain E3 produces epoxyalkanes from alkenes in an oxygen- and NADH-dependent reaction. The process of co-factor regeneration was studied by analysing the intracellular pools of NADH and storage material during the production of 1,2-epoxypropane from propene. With the depletion of NADH the production of 1,2-epoxypropane stopped. NADH could be regenerated from the oxidation of added co-substrate or from oxidation of storage material. Cells cultivated in chemostat culture under nitrogen limitation produced more 1,2-epoxypropane compared to cells cultivated under carbon limitation, due to their higher content of storage material. Addition of glucose to cells grown under carbon limitation stimulated the formation of 1,2-epoxypropane. The uptake of glucose resulted in the accumulation of storage material, which was utilized after depletion of the glucose. Glycogen and trehalose were the preferred forms of storage material used for co-factor regeneration. From the results it was concluded that formation and utilization of storage material play a crucial role in the process of co-factor regeneration in strain E3.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-12-3017
1993-12-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/12/mic-139-12-3017.html?itemId=/content/journal/micro/10.1099/00221287-139-12-3017&mimeType=html&fmt=ahah

References

  1. Antoine A. D., Tepper B. S. 1969a; Environmental control of glycogen and lipid content of Mycobacterium phlei. Journal of General Microbiology 55:217–226
    [Google Scholar]
  2. Antoine A.D., Tepper B.S. 1969b; Environmental control of glycogen and lipid content of Mycobacterium tuberculosis. Journal of Bacteriology 100:538–539
    [Google Scholar]
  3. Aisaka K., Ohshiro T, Uwajima T. 1992; Optimum culture conditions for the epoxidation of cis-propenylphosphonate to fosfomycin by Cellvibrio gilvus. Applied Microbiology and Biotechnology 36:431–435
    [Google Scholar]
  4. Bradford M.M. 1976; A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Burdon R.H. 1986; Techniques of lipidology; isolation, analysis and identification of lipids. In Laboratory Techniques in Biochemistry and Molecular Biology pp. 100–111 Burdon R.H., Van Knippenberg R.H. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  6. Elbein A.D., Mitchell M. 1973; Levels of glycogen and trehalose in Mycobacterium smegmatis and the purification and properties of the glycogen synthetase. Journal of Bacteriology 113:863–873
    [Google Scholar]
  7. Furuhashi K. 1986; A fermentation process for the production of optically active epoxides. Chemical Economy and Engineering Review 18:21–26
    [Google Scholar]
  8. Furuhashi K., Takagi M. 1984; Optimization of a medium for the production of 1,2-epoxytetradecane by Nocardia corallina B- 276. Applied Microbiology and Biotechnology 20:6–9
    [Google Scholar]
  9. Van Ginkel C.G., Welten H.G.J., De Bont J.A.M. 1987; Oxidation of gaseous and volatile hydrocarbons by selected alkene- utilizing bacteria. Applied and Environmental Microbiology 53:2903–2907
    [Google Scholar]
  10. Habets-Crützen A.Q.H., Brink L.E.S., Van Ginkel C.G., De Bont J.A.M., Tramper J. 1984; Production of epoxides from gaseous alkenes by resting-cell suspensions and immobilized cells of alkene-utilizing bacteria. Applied Microbiology and Biotechnology 20:245–250
    [Google Scholar]
  11. Habets-Crützen A.Q.H., De Bont J.A.M. 1985; Inactivation of alkene oxidation by epoxides in alkene- and alkane-grown bacteria. Applied Microbiology and Biotechnology 22:428–433
    [Google Scholar]
  12. Habets-Crützen A.Q.H., De Bont J.A.M. 1987; Effect of various co-substrates on 1,2-epoxypropane formation from propene by ethene-utilizing mycobacteria. Applied Microbiology and Biotechnology 26:434–438
    [Google Scholar]
  13. Hartmans S., Weber F.J., Somhorst D.P.M., De Bont J.A.M. 1991; Alkene monooxygenase from Mycobacterium: a multi-component enzyme. Journal of General Microbiology 137:2550–2560
    [Google Scholar]
  14. Johnstone S.L., Phillips G.T., Robertson B.W., Watts P.D., Bertola M.A., Koger H.S., Marx A.F. 1987; Stereoselective synthesis of S-(−)-β-blockers via microbially produced epoxide intermediates. In Biocatalysis in Organic Media pp. 387–392 Laane C., Tramper J., Lilly M. D. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  15. Van Laere A. 1989; Trehalose, reserve and/or stress metabolite?. FEMS Microbiology Reviews 63:201–210
    [Google Scholar]
  16. Passonneau J.V., Gatfield P.D., Schultz D.W., Lowry O.H. 1967; An enzymatic method for measurement of glycogen. Analytical Biochemistry 19:315–326
    [Google Scholar]
  17. Ratledge C. 1982; Nutrition, growth and metabolism. In The Biology of the Mycobacteria 1 pp. 185–274 Ratledge C., Stanford J. Edited by London: Academic Press;
    [Google Scholar]
  18. Seifter S., Dayton S., Novic B., Muntwyler E. 1950; The estimation of glycogen with anthrone reagent. Archives of Biochemistry 25:191–200
    [Google Scholar]
  19. Smith M.R., De Haan A., De Bont J.A.M. 1993; The effect of calcium alginate entrapment on the physiology of Mycobacterium sp. strain E3. Applied Microbiology and Biotechnology 38:642–648
    [Google Scholar]
  20. Stirling D.I., Dalton H. 1979; The fortuitous oxidation and cometabolism of various carbon compounds by whole-cell suspensions of Methylococcus capsulatus (Bath). FEMS Microbiology Letters 5:315–318
    [Google Scholar]
  21. Van Urk H., Postma E., Scheffers W.A., Van Dijken J.P. 1989; Glucose transport in Crabtree-positive and Crabtree-negative yeasts. Journal of General Microbiology 135:2399–2406
    [Google Scholar]
  22. Weber F.J., Van Berkel W.J.H., Hartmans S., De Bont J.A.M. 1992; Purification and properties of the NADH reductase component of alkene monooxygenase from Mycobacterium strain E3. Journal of Bacteriology 174:3275–3281
    [Google Scholar]
  23. Wiegant W.M., De Bont J.A.M. 1980; A new route for ethylene glycol metabolism in Mycobacterium E44. Journal of General Microbiology 120:325–331
    [Google Scholar]
  24. Winder F.G., Rooney S.A. 1970; Effect of nitrogenous components of the medium on the carbohydrate and nucleic acid content of Mycobacterium tuberculosis BCG. Journal of General Microbiology 63:29–39
    [Google Scholar]
  25. Yokobayashi K., Misaki A., Harada T. 1970; Purification and properties of Pseudomonas isoamylase. Biochimica et Biophvsica Acta 212:458–469
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-12-3017
Loading
/content/journal/micro/10.1099/00221287-139-12-3017
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error