1887

Abstract

This study was undertaken to search for the presence of immunoglobulin G (IgG)-binding proteins in , an important swine pathogen. Whole bacterial cells were incubated with human or pig IgG conjugated to gold particles and examined by transmission electron microscopy. Cells of some strains were labelled as were cells of the positive control strain, Cowan I. Binding of pig and human IgG to five different bacterial species of group D streptococci, to reference strains representing the 29 capsular types of , and to 12 capsular type 2 strains was then examined using Western blotting. All strains interacted with pig and human IgG, although the binding profiles were slightly different. A 52 kDa protein was observed in all capsular types of This protein, absent in other group D streptococcal species, was observed in all capsular type 2 isolates originating from diseased or clinically healthy pigs, and was shown to bind human IgG-Fc fragments. The IgG-binding activity was also observed in the culture supernatant and was sensitive to proteolysis.

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1993-12-01
2024-03-29
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References

  1. Arends J.P., Zanen H.C. 1988; Meningitis caused by Streptococcus suis in humans. Review of Infectious Diseases 10:131–137
    [Google Scholar]
  2. Beaudoin M., Harel J., Higgins R., Gottschalk M., Frenette M., Macinnes J.I. 1992; Molecular analysis of isolates of Streptococcus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe. Journal of General Microbiology 138:2639–2645
    [Google Scholar]
  3. Bendayan M. 1984; Protein A-gold electron microscopic immuno-chemistry: methods, applications, and limitations. Journal of Electron Microscopy Technique 1:243–270
    [Google Scholar]
  4. Björck L., Akerström. 1990; Streptococcal protein G. In Bacterial Immunoglobulin Binding Proteins 1 pp. 113–126 Edited by Boyle M.D.P. San Diego: Academic Press;
    [Google Scholar]
  5. Boyle M.D.P. 1990; The type I bacterial immunoglobulin-binding protein: staphylococcal protein A. In Bacterial Immunoglobulin Binding Proteins 1 pp. 17–28 Edited by Boyle M.D.P. San Diego: Academic Press;
    [Google Scholar]
  6. Brady L.J., Boyle M.D.P. 1990; Immunoglobulin A Fc-binding proteins associated with group B streptococci. In Bacterial Immunoglobulin Binding Proteins 1 pp. 201–224 Edited by Boyle M.D.P. San Diego: Academic Press;
    [Google Scholar]
  7. Brisebois L.M., Charlebois R., Higgins R., Nadeau M. 1990; Prevalence of Streptococcus suis in four to eight week old clinically healthy piglets. Canadian Journal of Veterinary Research 54:174–177
    [Google Scholar]
  8. Chhatwal G.S., Lämmler CH., Blobel H. 1985; Interactions of plasma proteins with group A, B, C, and G streptococci. Zentralblatt für Bakteriologie und Hygiene 259:219–227
    [Google Scholar]
  9. Christensen P., Kronvall G. 1974; Capacity of group A, B, C, D, and G streptococci to agglutinate sensitized sheep red cells. Acta Pathologica et Microbiologica Scandinavica 82:19–24
    [Google Scholar]
  10. Forsgren A., Sjöquist J. 1966; ‘Protein A’ from Staphylococcus aureus. I.Pseudo-immune reaction with human δ-globulin. Journal of Immunology 97:822–827
    [Google Scholar]
  11. Frens G. 1973; Controlled nucleation for the regulation of the particle in size in monodisperse gold suspension. Nature; London: 24120–22
    [Google Scholar]
  12. Gottschalk M., Higgins R., Jacques M., Beaudoin M., Henrichsen J. 1991; Characterization of six new capsular types (23-28) of Streptococcus suis. Journal of Clinical Microbiology 29:2590–2594
    [Google Scholar]
  13. Gottschalk M., Higgins R., Jacques M., Dubreuil D. 1992; Production and characterization of two Streptococcus suis capsular type 2 mutants. Veterinary Microbiology 30:59–71
    [Google Scholar]
  14. Higgins R., Gottschalk M. 1990; An update on Streptococcus suis identification. Journal of Veterinary Diagnostic Investigation 2:249–252
    [Google Scholar]
  15. Higgins R., Gottschalk M., Beaudoin M., Rawluk S.A. 1992; Distribution of Streptococcus suis capsular types in Quebec and western Canada. Canadian Veterinary Journal 33:27–30
    [Google Scholar]
  16. Higgins R., Gottschalk M., Fecteau G., Sauvageau R., Deguise S., Du Tremblay D. 1990; Isolation of Streptococcus suis from cattle. Canadian Veterinary Journal 31:529
    [Google Scholar]
  17. Hommez J., Wullepit J., Cassimon P. 1988; Streptococcus suis and other streptococcal species as a cause of extramammary infections in ruminants. Veterinary Record 123:626–627
    [Google Scholar]
  18. Jacques M., Gottschalk M., Foiry B., Higgins R. 1990; Ultrastructural study of surface components of Streptococcus suis. Journal of Bacteriology 172:2833–2838
    [Google Scholar]
  19. Kronvall G. 1973; A surface component in group A, C, and G streptococci with nonimmune reactivity for immunoglobulin G. Journal of Immunology 111:1401–1406
    [Google Scholar]
  20. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  21. Lämmler C., Schaufub P., Goretzki K., Blobel H. 1986; Screening for bacterial Fc-receptor activity on nitrocellulose mem-branes. Journal of Immunological Methods 90:47–50
    [Google Scholar]
  22. Lindahl G., Kronvall G. 1988; Non-immune binding of IgG to Clostridium perfringens. Preferential binding of IgM and aggregated IgG. Journal of Immunology 140:1223–1227
    [Google Scholar]
  23. Myhre E.B., Kronvall G. 1981; Immunoglobulin specifications of defined types of streptococcal Ig receptors. In Basic Concepts of Streptococci and Streptococcal Diseases Edited by Holm S.E., Christensen P. Chertsey, Surrey: Reedbooks Ltd.;
    [Google Scholar]
  24. Reader R.A., Faulmann E.L., Boyle M.D.P. 1991; Evidence for functional heterogeneity in IgG Fc-binding proteins associated with group A streptococci. Journal of Immunology 146:1247–1253
    [Google Scholar]
  25. Reis K.J., Siden J., Boyle M.D.P. 1988; Selective colony blotting to expand bacterial surface receptors: applications to receptors for rat immunoglobulins. Biotechniques 6:130–136
    [Google Scholar]
  26. Sanford S.E., Higgins R. 1992; Streptococcal Diseases. In Diseases of Swine pp. 588–598 Edited by Leman A.D., Straw B.E., Mengeling W.L., D’allaire S., Taylor D.J. Ames, Iowa: Iowa State University Press;
    [Google Scholar]
  27. Sawa Y., Watanabe T., Shibata K. 1992; Immunoglobulin G Fc fragment-binding proteins in Mycoplasma salivarium cells. Microbiology and Immunology 36:655–659
    [Google Scholar]
  28. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4353
    [Google Scholar]
  29. Trottier S., Higgins R., Brochu G., Gottschalk M. 1991; A case of human endocarditis due to Streptococcus suis in North America. Review of Infectious Diseases 13:1251–1252
    [Google Scholar]
  30. Vecht U., Wisselink H.J., Jellema M.L., Smith H.E. 1991; Identification of two proteins associated with virulence of Strep¬tococcus suis type 2. Infection and Immunity 59:3156–3162
    [Google Scholar]
  31. Widders P.R. 1990; Fc receptors and the pathogenesis of bacterial infections in animals. In Bacterial Immunoglobulin Binding Proteins 1 pp. 375–396 Edited by Boyle M.D.P. San Diego: Academic Press;
    [Google Scholar]
  32. Yarnall M., Widders P.R. 1990; Type V Fc receptor from Streptococcus zooepidemicus. In Bacterial Immunoglobulin Binding Proteins 1 pp. 155–164 Edited by Boyle M.D.P. San Diego: Academic Press;
    [Google Scholar]
  33. Yarnall M., Widders P.R., Corbeil L.B. 1988; Isolation and characterization of Fc receptors from Haemophilus somnus. Scandinavian Journal of Immunology 28:129–137
    [Google Scholar]
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